Dynamics of Intrapulmonary Bacterial Growth in a Murine Model of Repeated Microaspiration

To study the change in intrapulmonary bacterial growth rate over time during Gram-negative pneumonia, a two-hit model of recurrent bacterial aspiration was developed in mice. A mutant of Klebsiella pneumoniae was isolated that could be distinguished from the wild type when cultured on appropriate me...

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Veröffentlicht in:American journal of respiratory cell and molecular biology 2005-11, Vol.33 (5), p.476-482
Hauptverfasser: Ben-David, Itzhak, Price, Sarah E, Bortz, David M, Greineder, Colin F, Cohen, Samuel E, Bauer, Amy L, Jackson, Trachette L, Younger, John G
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container_end_page 482
container_issue 5
container_start_page 476
container_title American journal of respiratory cell and molecular biology
container_volume 33
creator Ben-David, Itzhak
Price, Sarah E
Bortz, David M
Greineder, Colin F
Cohen, Samuel E
Bauer, Amy L
Jackson, Trachette L
Younger, John G
description To study the change in intrapulmonary bacterial growth rate over time during Gram-negative pneumonia, a two-hit model of recurrent bacterial aspiration was developed in mice. A mutant of Klebsiella pneumoniae was isolated that could be distinguished from the wild type when cultured on appropriate media. These strains were intranasally administered, 4 h apart, to mice whose lungs were quantitatively cultured 24 h later. The relative burden of each aspirated inoculum was determined, and, using the administered dose and the number of bacteria from each inoculum present at the end of the experiment, first-order growth constants for each inoculum were calculated. Results indicate that after an initial aspiration of this organism, subsequently aspirated bacteria proliferate more slowly. When two aspirations occurred 4 h apart, the bacteria aspirated first represented 96% of total lung burden at 24 h. The growth constant of the second inoculum was related to the magnitude of the first inoculum in an inverse, nonlinear fashion. When parallel experiments were performed in complement C3-deficient mice, no suppression of the second inoculum was noted, suggesting that early upregulation of antibacterial activity in the lung is a C3-mediated event.
doi_str_mv 10.1165/rcmb.2005-0053OC
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subjects Animals
Complement C3 - genetics
Disease Models, Animal
Galactose - genetics
Klebsiella Infections - microbiology
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - growth & development
Lung - microbiology
Mice
Mice, Mutant Strains
Mutation
Pneumonia, Bacterial - microbiology
Serratia Infections - microbiology
Serratia marcescens - growth & development
title Dynamics of Intrapulmonary Bacterial Growth in a Murine Model of Repeated Microaspiration
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