TRPV1 channels control synaptic plasticity in the developing superior colliculus
Long-term depression (LTD) in the rodent superior colliculus (SC) is regarded as a model of synaptic refinement because it can be induced during development but not in adults. We investigated the role of transient receptor potential vanilloid type-1 (TRPV1) channels in this type of synaptic plastici...
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| Veröffentlicht in: | The Journal of physiology 2009-06, Vol.587 (11), p.2521-2535 |
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| creator | Maione, Sabatino Cristino, Luigia Migliozzi, Anna Lucia Georgiou, Anne L. Starowicz, Katarzyna Salt, Thomas E. Di Marzo, Vincenzo |
| description | Long-term depression (LTD) in the rodent superior colliculus (SC) is regarded as a model of synaptic refinement because it
can be induced during development but not in adults. We investigated the role of transient receptor potential vanilloid type-1
(TRPV1) channels in this type of synaptic plasticity. Experiments were carried out in pigmented mice aged between postnatal
day 8 (P8) and 42 (P42) and in adult mice. Retinal axons to the SC were labelled by injection of cholera toxin-β (CTβ) into
the eye. Immunohistochemical staining for CTβ, TRPV1 and markers of glutamatergic and GABAergic cells and fibres (VGLUT1 and
VGAT or GAD65, respectively) was performed by using multiple immunofluorescence. This showed that both glutamatergic retinal
afferents to, and some GABAergic neurones in, the superficial SC are TRPV1 positive in juvenile but not adult mice. Field
potential recordings were made from the superficial grey layer in parasagittal SC slices, and LTD (76 ± 8% of control responses)
was induced with a 50 Hz, 20 s tetanus. Activation of TRPV1 with resiniferatoxin also reduced field potential amplitude to
84 ± 8% of control values. Blockade of TRPV1 with the selective antagonist 5â²-iodo-resiniferatoxin prevented the induction
of LTD (98 ± 4% of control values), but did not cause its reversal if LTD was already established. N -acylphosphatidylethanolamine-specific phospholipase D and 12-lipoxygenase, two proposed endovanilloid biosynthesizing enzymes,
were co-expressed with TRPV1 in the SC at P14 and P28. These results suggest that TRPV1 modulates retinocollicular responses
in the developing SC and is activated during tetanic stimulation by endovanilloid ligands to participate in the induction
of LTD. |
| doi_str_mv | 10.1113/jphysiol.2009.171900 |
| format | Article |
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can be induced during development but not in adults. We investigated the role of transient receptor potential vanilloid type-1
(TRPV1) channels in this type of synaptic plasticity. Experiments were carried out in pigmented mice aged between postnatal
day 8 (P8) and 42 (P42) and in adult mice. Retinal axons to the SC were labelled by injection of cholera toxin-β (CTβ) into
the eye. Immunohistochemical staining for CTβ, TRPV1 and markers of glutamatergic and GABAergic cells and fibres (VGLUT1 and
VGAT or GAD65, respectively) was performed by using multiple immunofluorescence. This showed that both glutamatergic retinal
afferents to, and some GABAergic neurones in, the superficial SC are TRPV1 positive in juvenile but not adult mice. Field
potential recordings were made from the superficial grey layer in parasagittal SC slices, and LTD (76 ± 8% of control responses)
was induced with a 50 Hz, 20 s tetanus. Activation of TRPV1 with resiniferatoxin also reduced field potential amplitude to
84 ± 8% of control values. Blockade of TRPV1 with the selective antagonist 5â²-iodo-resiniferatoxin prevented the induction
of LTD (98 ± 4% of control values), but did not cause its reversal if LTD was already established. N -acylphosphatidylethanolamine-specific phospholipase D and 12-lipoxygenase, two proposed endovanilloid biosynthesizing enzymes,
were co-expressed with TRPV1 in the SC at P14 and P28. These results suggest that TRPV1 modulates retinocollicular responses
in the developing SC and is activated during tetanic stimulation by endovanilloid ligands to participate in the induction
of LTD.</description><identifier>ISSN: 0022-3751</identifier><identifier>ISSN: 1469-7793</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.2009.171900</identifier><identifier>PMID: 19406878</identifier><language>eng</language><publisher>Oxford, UK: The Physiological Society</publisher><subject>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism ; Age Factors ; Aging - metabolism ; Animals ; Arachidonate 12-Lipoxygenase - metabolism ; Diterpenes - pharmacology ; Ethanolamines - metabolism ; Excitatory Postsynaptic Potentials ; Glutamate Decarboxylase - metabolism ; In Vitro Techniques ; Ligands ; Long-Term Synaptic Depression - drug effects ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Fluorescence ; Neurons - drug effects ; Neurons - enzymology ; Neurons - metabolism ; Neuroscience ; Phospholipase D - metabolism ; Staining and Labeling - methods ; Superior Colliculi - drug effects ; Superior Colliculi - growth & development ; Superior Colliculi - metabolism ; Synaptic Transmission - drug effects ; Time Factors ; TRPV Cation Channels - drug effects ; TRPV Cation Channels - metabolism ; Vesicular Glutamate Transport Protein 1 - metabolism ; Vesicular Inhibitory Amino Acid Transport Proteins - metabolism</subject><ispartof>The Journal of physiology, 2009-06, Vol.587 (11), p.2521-2535</ispartof><rights>2009 The Authors. Journal compilation © 2009 The Physiological Society</rights><rights>Journal compilation © 2009 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6038-42355036a1ffa1fdec5e17b13bb121459cb6a916114e420fe4d450a28ff0e3e73</citedby><cites>FETCH-LOGICAL-c6038-42355036a1ffa1fdec5e17b13bb121459cb6a916114e420fe4d450a28ff0e3e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2714018/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2714018/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,1418,1434,27928,27929,45578,45579,46413,46837,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19406878$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maione, Sabatino</creatorcontrib><creatorcontrib>Cristino, Luigia</creatorcontrib><creatorcontrib>Migliozzi, Anna Lucia</creatorcontrib><creatorcontrib>Georgiou, Anne L.</creatorcontrib><creatorcontrib>Starowicz, Katarzyna</creatorcontrib><creatorcontrib>Salt, Thomas E.</creatorcontrib><creatorcontrib>Di Marzo, Vincenzo</creatorcontrib><title>TRPV1 channels control synaptic plasticity in the developing superior colliculus</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>Long-term depression (LTD) in the rodent superior colliculus (SC) is regarded as a model of synaptic refinement because it
can be induced during development but not in adults. We investigated the role of transient receptor potential vanilloid type-1
(TRPV1) channels in this type of synaptic plasticity. Experiments were carried out in pigmented mice aged between postnatal
day 8 (P8) and 42 (P42) and in adult mice. Retinal axons to the SC were labelled by injection of cholera toxin-β (CTβ) into
the eye. Immunohistochemical staining for CTβ, TRPV1 and markers of glutamatergic and GABAergic cells and fibres (VGLUT1 and
VGAT or GAD65, respectively) was performed by using multiple immunofluorescence. This showed that both glutamatergic retinal
afferents to, and some GABAergic neurones in, the superficial SC are TRPV1 positive in juvenile but not adult mice. Field
potential recordings were made from the superficial grey layer in parasagittal SC slices, and LTD (76 ± 8% of control responses)
was induced with a 50 Hz, 20 s tetanus. Activation of TRPV1 with resiniferatoxin also reduced field potential amplitude to
84 ± 8% of control values. Blockade of TRPV1 with the selective antagonist 5â²-iodo-resiniferatoxin prevented the induction
of LTD (98 ± 4% of control values), but did not cause its reversal if LTD was already established. N -acylphosphatidylethanolamine-specific phospholipase D and 12-lipoxygenase, two proposed endovanilloid biosynthesizing enzymes,
were co-expressed with TRPV1 in the SC at P14 and P28. These results suggest that TRPV1 modulates retinocollicular responses
in the developing SC and is activated during tetanic stimulation by endovanilloid ligands to participate in the induction
of LTD.</description><subject>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism</subject><subject>Age Factors</subject><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Arachidonate 12-Lipoxygenase - metabolism</subject><subject>Diterpenes - pharmacology</subject><subject>Ethanolamines - metabolism</subject><subject>Excitatory Postsynaptic Potentials</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>In Vitro Techniques</subject><subject>Ligands</subject><subject>Long-Term Synaptic Depression - drug effects</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Fluorescence</subject><subject>Neurons - drug effects</subject><subject>Neurons - enzymology</subject><subject>Neurons - metabolism</subject><subject>Neuroscience</subject><subject>Phospholipase D - metabolism</subject><subject>Staining and Labeling - methods</subject><subject>Superior Colliculi - drug effects</subject><subject>Superior Colliculi - growth & development</subject><subject>Superior Colliculi - metabolism</subject><subject>Synaptic Transmission - drug effects</subject><subject>Time Factors</subject><subject>TRPV Cation Channels - drug effects</subject><subject>TRPV Cation Channels - metabolism</subject><subject>Vesicular Glutamate Transport Protein 1 - metabolism</subject><subject>Vesicular Inhibitory Amino Acid Transport Proteins - metabolism</subject><issn>0022-3751</issn><issn>1469-7793</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuP0zAUhS0EYsrAP0AoKyQWKb5-xPEGCY14aiQqVNharnvTeOTGIU5mlH-Pq5TXillYd-HvHJ17DyHPga4BgL--6ds5-RjWjFK9BgWa0gdkBaLSpVKaPyQrShkruZJwQZ6kdEMpcKr1Y3IBWtCqVvWKbLZfN9-hcK3tOgypcLEbhxiKNHe2H70r-mBTnn6cC98VY4vFHm8xxN53hyJNPQ4-DlkWgndTmNJT8qixIeGz87wk396_2159LK-_fPh09fa6dBXldSkYl5LyykLT5LdHJxHUDvhuBwyE1G5XWQ0VgEDBaINiLyS1rG4aihwVvyRvFt9-2h1x7zDntsH0gz_aYTbRevPvT-dbc4i3hikQFOps8PJsMMQfE6bRHH1yGILtME7JVIoDEwz-C-b7K1pRcR-wkkydsosFdENMacDmd2yg5lSu-VXuSaPNUm6Wvfh75T-ic5sZ0Atw5wPO9zI1288bLuRJ-2rRtv7Q3vkBzUKn6DyOs5G1yi6GyXyRny7FxJA</recordid><startdate>200906</startdate><enddate>200906</enddate><creator>Maione, Sabatino</creator><creator>Cristino, Luigia</creator><creator>Migliozzi, Anna Lucia</creator><creator>Georgiou, Anne L.</creator><creator>Starowicz, Katarzyna</creator><creator>Salt, Thomas E.</creator><creator>Di Marzo, Vincenzo</creator><general>The Physiological Society</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200906</creationdate><title>TRPV1 channels control synaptic plasticity in the developing superior colliculus</title><author>Maione, Sabatino ; Cristino, Luigia ; Migliozzi, Anna Lucia ; Georgiou, Anne L. ; Starowicz, Katarzyna ; Salt, Thomas E. ; Di Marzo, Vincenzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6038-42355036a1ffa1fdec5e17b13bb121459cb6a916114e420fe4d450a28ff0e3e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism</topic><topic>Age Factors</topic><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Arachidonate 12-Lipoxygenase - metabolism</topic><topic>Diterpenes - pharmacology</topic><topic>Ethanolamines - metabolism</topic><topic>Excitatory Postsynaptic Potentials</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>In Vitro Techniques</topic><topic>Ligands</topic><topic>Long-Term Synaptic Depression - drug effects</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microscopy, Fluorescence</topic><topic>Neurons - drug effects</topic><topic>Neurons - enzymology</topic><topic>Neurons - metabolism</topic><topic>Neuroscience</topic><topic>Phospholipase D - metabolism</topic><topic>Staining and Labeling - methods</topic><topic>Superior Colliculi - drug effects</topic><topic>Superior Colliculi - growth & development</topic><topic>Superior Colliculi - metabolism</topic><topic>Synaptic Transmission - drug effects</topic><topic>Time Factors</topic><topic>TRPV Cation Channels - drug effects</topic><topic>TRPV Cation Channels - metabolism</topic><topic>Vesicular Glutamate Transport Protein 1 - metabolism</topic><topic>Vesicular Inhibitory Amino Acid Transport Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maione, Sabatino</creatorcontrib><creatorcontrib>Cristino, Luigia</creatorcontrib><creatorcontrib>Migliozzi, Anna Lucia</creatorcontrib><creatorcontrib>Georgiou, Anne L.</creatorcontrib><creatorcontrib>Starowicz, Katarzyna</creatorcontrib><creatorcontrib>Salt, Thomas E.</creatorcontrib><creatorcontrib>Di Marzo, Vincenzo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maione, Sabatino</au><au>Cristino, Luigia</au><au>Migliozzi, Anna Lucia</au><au>Georgiou, Anne L.</au><au>Starowicz, Katarzyna</au><au>Salt, Thomas E.</au><au>Di Marzo, Vincenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRPV1 channels control synaptic plasticity in the developing superior colliculus</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2009-06</date><risdate>2009</risdate><volume>587</volume><issue>11</issue><spage>2521</spage><epage>2535</epage><pages>2521-2535</pages><issn>0022-3751</issn><issn>1469-7793</issn><eissn>1469-7793</eissn><abstract>Long-term depression (LTD) in the rodent superior colliculus (SC) is regarded as a model of synaptic refinement because it
can be induced during development but not in adults. We investigated the role of transient receptor potential vanilloid type-1
(TRPV1) channels in this type of synaptic plasticity. Experiments were carried out in pigmented mice aged between postnatal
day 8 (P8) and 42 (P42) and in adult mice. Retinal axons to the SC were labelled by injection of cholera toxin-β (CTβ) into
the eye. Immunohistochemical staining for CTβ, TRPV1 and markers of glutamatergic and GABAergic cells and fibres (VGLUT1 and
VGAT or GAD65, respectively) was performed by using multiple immunofluorescence. This showed that both glutamatergic retinal
afferents to, and some GABAergic neurones in, the superficial SC are TRPV1 positive in juvenile but not adult mice. Field
potential recordings were made from the superficial grey layer in parasagittal SC slices, and LTD (76 ± 8% of control responses)
was induced with a 50 Hz, 20 s tetanus. Activation of TRPV1 with resiniferatoxin also reduced field potential amplitude to
84 ± 8% of control values. Blockade of TRPV1 with the selective antagonist 5â²-iodo-resiniferatoxin prevented the induction
of LTD (98 ± 4% of control values), but did not cause its reversal if LTD was already established. N -acylphosphatidylethanolamine-specific phospholipase D and 12-lipoxygenase, two proposed endovanilloid biosynthesizing enzymes,
were co-expressed with TRPV1 in the SC at P14 and P28. These results suggest that TRPV1 modulates retinocollicular responses
in the developing SC and is activated during tetanic stimulation by endovanilloid ligands to participate in the induction
of LTD.</abstract><cop>Oxford, UK</cop><pub>The Physiological Society</pub><pmid>19406878</pmid><doi>10.1113/jphysiol.2009.171900</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism Age Factors Aging - metabolism Animals Arachidonate 12-Lipoxygenase - metabolism Diterpenes - pharmacology Ethanolamines - metabolism Excitatory Postsynaptic Potentials Glutamate Decarboxylase - metabolism In Vitro Techniques Ligands Long-Term Synaptic Depression - drug effects Male Mice Mice, Inbred C57BL Microscopy, Fluorescence Neurons - drug effects Neurons - enzymology Neurons - metabolism Neuroscience Phospholipase D - metabolism Staining and Labeling - methods Superior Colliculi - drug effects Superior Colliculi - growth & development Superior Colliculi - metabolism Synaptic Transmission - drug effects Time Factors TRPV Cation Channels - drug effects TRPV Cation Channels - metabolism Vesicular Glutamate Transport Protein 1 - metabolism Vesicular Inhibitory Amino Acid Transport Proteins - metabolism |
| title | TRPV1 channels control synaptic plasticity in the developing superior colliculus |
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