Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules
Background: Carcinoma in situ (CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the...
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| Veröffentlicht in: | British journal of cancer 2009-07, Vol.101 (1), p.64-70 |
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| creator | Silván, U Arlucea, J Andrade, R Díez-Torre, A Silió, M Konerding, M A Aréchaga, J |
| description | Background:
Carcinoma
in situ
(CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed to date.
METHODS:
We have developed a tumour model that involves the microinjection of green fluorescent protein-labelled embryonic stem (ES) cells (which are functionally equivalent to CIS cells) into syngenic mouse seminiferous tubules, a unique cell microenvironment in which germinal cells mature and CIS arise. To characterise the vascularisation of teratocarcinomas, which arise after cell transplant, we used immunohistochemistry, together with a qualitative and quantitative analysis of scanning electron microscopy images of corrosion casting samples.
Results:
Embryonic stem cells transplanted into seminiferous tubules did not differentiate into germinal cells, but rather they behaved as invasive embryonal carcinoma (EC) stem cells. The vascular pattern of the experimental teratocarcinomas showed a highly disorganised architecture, and some of the neoplastic capillaries were derived, at least in part, from the original transplanted ES cells.
Conclusion:
The transplantation of pluripotent ES cells into seminiferous tubules efficiently recapitulates the early stages of development of teratocarcinomas. Consequently, this method constitutes a novel
in vivo
model to study the mechanisms of invasion and progression of experimental germinal tumours. |
| doi_str_mv | 10.1038/sj.bjc.6605125 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2713705</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>745715902</sourcerecordid><originalsourceid>FETCH-LOGICAL-c517t-763c086bf375398b704826b9524eb066ee98ecd0df78f8be763a42d3a3ccb0143</originalsourceid><addsrcrecordid>eNp1kc1rFTEUxYMo9rW6dSlBkK7eaz4mk8xGKMUvKLjRdUgyd54ZZ5Jnkqn0vzePN7QquArh_nJyzj0IvaJkRwlXV3nc2dHt2pYIysQTtKGCsy1VTD5FG0KI3JKOkTN0nvNYrx1R8jk6o52gnMhmg9J12Pu4hwDZZ2xCj-9MdstkEg5QfsX0A8cBF0imRGeS8yHOBg8pzhhmm-5j8A7nAjN2ME24JBPyYTKhYB9KxBlmH_wAKS4Zl8UuE-QX6Nlgpgwv1_MCffvw_uvNp-3tl4-fb65vt05QWbay5Y6o1g5cCt4pK0mjWGs7wRqwpG0BOgWuJ_0g1aAsVN40rOeGO2cJbfgFenfSPSx2ht5BqO4mfUh-NuleR-P135Pgv-t9vNNMUi6JqAKXq0CKPxfIRc8-H2OaADWPlo2QVHSEVfLNP-QYlxRqOs14XXvDOlKh3QlyKeacYHiwQok-lqnzqGuZei2zPnj9Z4BHfG2vAm9XoHZmpqEu3_n8wDHaccX4kbs6cbmOwh7So73_fP0bvF67eg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>230004290</pqid></control><display><type>article</type><title>Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Nature Journals Online</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Silván, U ; Arlucea, J ; Andrade, R ; Díez-Torre, A ; Silió, M ; Konerding, M A ; Aréchaga, J</creator><creatorcontrib>Silván, U ; Arlucea, J ; Andrade, R ; Díez-Torre, A ; Silió, M ; Konerding, M A ; Aréchaga, J</creatorcontrib><description>Background:
Carcinoma
in situ
(CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed to date.
METHODS:
We have developed a tumour model that involves the microinjection of green fluorescent protein-labelled embryonic stem (ES) cells (which are functionally equivalent to CIS cells) into syngenic mouse seminiferous tubules, a unique cell microenvironment in which germinal cells mature and CIS arise. To characterise the vascularisation of teratocarcinomas, which arise after cell transplant, we used immunohistochemistry, together with a qualitative and quantitative analysis of scanning electron microscopy images of corrosion casting samples.
Results:
Embryonic stem cells transplanted into seminiferous tubules did not differentiate into germinal cells, but rather they behaved as invasive embryonal carcinoma (EC) stem cells. The vascular pattern of the experimental teratocarcinomas showed a highly disorganised architecture, and some of the neoplastic capillaries were derived, at least in part, from the original transplanted ES cells.
Conclusion:
The transplantation of pluripotent ES cells into seminiferous tubules efficiently recapitulates the early stages of development of teratocarcinomas. Consequently, this method constitutes a novel
in vivo
model to study the mechanisms of invasion and progression of experimental germinal tumours.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/sj.bjc.6605125</identifier><identifier>PMID: 19513074</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Angiogenesis ; Animals ; Biological and medical sciences ; Biology ; Biomedical and Life Sciences ; Biomedicine ; Blood vessels ; Cancer Research ; Cell Transformation, Neoplastic - pathology ; Drug Resistance ; Embryonal Carcinoma Stem Cells - pathology ; Epidemiology ; Histology ; Male ; Medical research ; Medical sciences ; Mice ; Microscopy ; Molecular Medicine ; Neovascularization, Pathologic - pathology ; Oncology ; Pluripotent Stem Cells - pathology ; Pluripotent Stem Cells - transplantation ; Seminiferous Tubules - pathology ; Stem Cell Transplantation ; Teratocarcinoma - blood supply ; Teratocarcinoma - pathology ; Testicular Neoplasms - blood supply ; Testicular Neoplasms - pathology ; Translational Therapeutics ; Tumors</subject><ispartof>British journal of cancer, 2009-07, Vol.101 (1), p.64-70</ispartof><rights>The Author(s) 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jul 7, 2009</rights><rights>Copyright © 2009 Cancer Research UK 2009 Cancer Research UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-763c086bf375398b704826b9524eb066ee98ecd0df78f8be763a42d3a3ccb0143</citedby><cites>FETCH-LOGICAL-c517t-763c086bf375398b704826b9524eb066ee98ecd0df78f8be763a42d3a3ccb0143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713705/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713705/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21938234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19513074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silván, U</creatorcontrib><creatorcontrib>Arlucea, J</creatorcontrib><creatorcontrib>Andrade, R</creatorcontrib><creatorcontrib>Díez-Torre, A</creatorcontrib><creatorcontrib>Silió, M</creatorcontrib><creatorcontrib>Konerding, M A</creatorcontrib><creatorcontrib>Aréchaga, J</creatorcontrib><title>Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Background:
Carcinoma
in situ
(CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed to date.
METHODS:
We have developed a tumour model that involves the microinjection of green fluorescent protein-labelled embryonic stem (ES) cells (which are functionally equivalent to CIS cells) into syngenic mouse seminiferous tubules, a unique cell microenvironment in which germinal cells mature and CIS arise. To characterise the vascularisation of teratocarcinomas, which arise after cell transplant, we used immunohistochemistry, together with a qualitative and quantitative analysis of scanning electron microscopy images of corrosion casting samples.
Results:
Embryonic stem cells transplanted into seminiferous tubules did not differentiate into germinal cells, but rather they behaved as invasive embryonal carcinoma (EC) stem cells. The vascular pattern of the experimental teratocarcinomas showed a highly disorganised architecture, and some of the neoplastic capillaries were derived, at least in part, from the original transplanted ES cells.
Conclusion:
The transplantation of pluripotent ES cells into seminiferous tubules efficiently recapitulates the early stages of development of teratocarcinomas. Consequently, this method constitutes a novel
in vivo
model to study the mechanisms of invasion and progression of experimental germinal tumours.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood vessels</subject><subject>Cancer Research</subject><subject>Cell Transformation, Neoplastic - pathology</subject><subject>Drug Resistance</subject><subject>Embryonal Carcinoma Stem Cells - pathology</subject><subject>Epidemiology</subject><subject>Histology</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Molecular Medicine</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Oncology</subject><subject>Pluripotent Stem Cells - pathology</subject><subject>Pluripotent Stem Cells - transplantation</subject><subject>Seminiferous Tubules - pathology</subject><subject>Stem Cell Transplantation</subject><subject>Teratocarcinoma - blood supply</subject><subject>Teratocarcinoma - pathology</subject><subject>Testicular Neoplasms - blood supply</subject><subject>Testicular Neoplasms - pathology</subject><subject>Translational Therapeutics</subject><subject>Tumors</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc1rFTEUxYMo9rW6dSlBkK7eaz4mk8xGKMUvKLjRdUgyd54ZZ5Jnkqn0vzePN7QquArh_nJyzj0IvaJkRwlXV3nc2dHt2pYIysQTtKGCsy1VTD5FG0KI3JKOkTN0nvNYrx1R8jk6o52gnMhmg9J12Pu4hwDZZ2xCj-9MdstkEg5QfsX0A8cBF0imRGeS8yHOBg8pzhhmm-5j8A7nAjN2ME24JBPyYTKhYB9KxBlmH_wAKS4Zl8UuE-QX6Nlgpgwv1_MCffvw_uvNp-3tl4-fb65vt05QWbay5Y6o1g5cCt4pK0mjWGs7wRqwpG0BOgWuJ_0g1aAsVN40rOeGO2cJbfgFenfSPSx2ht5BqO4mfUh-NuleR-P135Pgv-t9vNNMUi6JqAKXq0CKPxfIRc8-H2OaADWPlo2QVHSEVfLNP-QYlxRqOs14XXvDOlKh3QlyKeacYHiwQok-lqnzqGuZei2zPnj9Z4BHfG2vAm9XoHZmpqEu3_n8wDHaccX4kbs6cbmOwh7So73_fP0bvF67eg</recordid><startdate>20090707</startdate><enddate>20090707</enddate><creator>Silván, U</creator><creator>Arlucea, J</creator><creator>Andrade, R</creator><creator>Díez-Torre, A</creator><creator>Silió, M</creator><creator>Konerding, M A</creator><creator>Aréchaga, J</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20090707</creationdate><title>Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules</title><author>Silván, U ; Arlucea, J ; Andrade, R ; Díez-Torre, A ; Silió, M ; Konerding, M A ; Aréchaga, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-763c086bf375398b704826b9524eb066ee98ecd0df78f8be763a42d3a3ccb0143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood vessels</topic><topic>Cancer Research</topic><topic>Cell Transformation, Neoplastic - pathology</topic><topic>Drug Resistance</topic><topic>Embryonal Carcinoma Stem Cells - pathology</topic><topic>Epidemiology</topic><topic>Histology</topic><topic>Male</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Molecular Medicine</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Oncology</topic><topic>Pluripotent Stem Cells - pathology</topic><topic>Pluripotent Stem Cells - transplantation</topic><topic>Seminiferous Tubules - pathology</topic><topic>Stem Cell Transplantation</topic><topic>Teratocarcinoma - blood supply</topic><topic>Teratocarcinoma - pathology</topic><topic>Testicular Neoplasms - blood supply</topic><topic>Testicular Neoplasms - pathology</topic><topic>Translational Therapeutics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silván, U</creatorcontrib><creatorcontrib>Arlucea, J</creatorcontrib><creatorcontrib>Andrade, R</creatorcontrib><creatorcontrib>Díez-Torre, A</creatorcontrib><creatorcontrib>Silió, M</creatorcontrib><creatorcontrib>Konerding, M A</creatorcontrib><creatorcontrib>Aréchaga, J</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silván, U</au><au>Arlucea, J</au><au>Andrade, R</au><au>Díez-Torre, A</au><au>Silió, M</au><au>Konerding, M A</au><au>Aréchaga, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2009-07-07</date><risdate>2009</risdate><volume>101</volume><issue>1</issue><spage>64</spage><epage>70</epage><pages>64-70</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>Background:
Carcinoma
in situ
(CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed to date.
METHODS:
We have developed a tumour model that involves the microinjection of green fluorescent protein-labelled embryonic stem (ES) cells (which are functionally equivalent to CIS cells) into syngenic mouse seminiferous tubules, a unique cell microenvironment in which germinal cells mature and CIS arise. To characterise the vascularisation of teratocarcinomas, which arise after cell transplant, we used immunohistochemistry, together with a qualitative and quantitative analysis of scanning electron microscopy images of corrosion casting samples.
Results:
Embryonic stem cells transplanted into seminiferous tubules did not differentiate into germinal cells, but rather they behaved as invasive embryonal carcinoma (EC) stem cells. The vascular pattern of the experimental teratocarcinomas showed a highly disorganised architecture, and some of the neoplastic capillaries were derived, at least in part, from the original transplanted ES cells.
Conclusion:
The transplantation of pluripotent ES cells into seminiferous tubules efficiently recapitulates the early stages of development of teratocarcinomas. Consequently, this method constitutes a novel
in vivo
model to study the mechanisms of invasion and progression of experimental germinal tumours.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>19513074</pmid><doi>10.1038/sj.bjc.6605125</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | British journal of cancer, 2009-07, Vol.101 (1), p.64-70 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Angiogenesis Animals Biological and medical sciences Biology Biomedical and Life Sciences Biomedicine Blood vessels Cancer Research Cell Transformation, Neoplastic - pathology Drug Resistance Embryonal Carcinoma Stem Cells - pathology Epidemiology Histology Male Medical research Medical sciences Mice Microscopy Molecular Medicine Neovascularization, Pathologic - pathology Oncology Pluripotent Stem Cells - pathology Pluripotent Stem Cells - transplantation Seminiferous Tubules - pathology Stem Cell Transplantation Teratocarcinoma - blood supply Teratocarcinoma - pathology Testicular Neoplasms - blood supply Testicular Neoplasms - pathology Translational Therapeutics Tumors |
| title | Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules |
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