Rheological Properties of Cross-Linked Hyaluronan-Gelatin Hydrogels for Tissue Engineering
Hydrogels that mimic the natural extracellular matrix (ECM) are used in three‐dimensional cell culture, cell therapy, and tissue engineering. A semi‐synthetic ECM based on cross‐linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM...
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| Veröffentlicht in: | Macromolecular bioscience 2009-01, Vol.9 (1), p.20-28 |
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| creator | Vanderhooft, Janssen L. Alcoutlabi, Mataz Magda, Jules J. Prestwich, Glenn D. |
| description | Hydrogels that mimic the natural extracellular matrix (ECM) are used in three‐dimensional cell culture, cell therapy, and tissue engineering. A semi‐synthetic ECM based on cross‐linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross‐linking density were the main determinants of gel stiffness. Increase in the ratio of thiol‐modified gelatin reduced gel stiffness by diluting the effective concentration of the HA component. |
| doi_str_mv | 10.1002/mabi.200800141 |
| format | Article |
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A semi‐synthetic ECM based on cross‐linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross‐linking density were the main determinants of gel stiffness. Increase in the ratio of thiol‐modified gelatin reduced gel stiffness by diluting the effective concentration of the HA component.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.200800141</identifier><identifier>PMID: 18839402</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Applied sciences ; Biocompatible Materials - chemistry ; Biological and medical sciences ; biomaterials ; Cross-Linking Reagents - chemistry ; Elasticity ; Exact sciences and technology ; Extracellular Matrix - chemistry ; Gelatin - chemistry ; hyaluronic acid ; Hyaluronic Acid - chemistry ; hydrogels ; Hydrogels - chemistry ; Materials Testing ; Medical sciences ; Molecular Structure ; Natural polymers ; Physicochemistry of polymers ; Proteins ; Rheology ; Shear Strength ; Starch and polysaccharides ; Stress, Mechanical ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; tissue engineering ; Tissue Engineering - methods</subject><ispartof>Macromolecular bioscience, 2009-01, Vol.9 (1), p.20-28</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2009 INIST-CNRS</rights><rights>2009 WILEY-VCH Verlag GmbH & Co. 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Biosci</addtitle><description>Hydrogels that mimic the natural extracellular matrix (ECM) are used in three‐dimensional cell culture, cell therapy, and tissue engineering. A semi‐synthetic ECM based on cross‐linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross‐linking density were the main determinants of gel stiffness. Increase in the ratio of thiol‐modified gelatin reduced gel stiffness by diluting the effective concentration of the HA component.</description><subject>Applied sciences</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological and medical sciences</subject><subject>biomaterials</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Elasticity</subject><subject>Exact sciences and technology</subject><subject>Extracellular Matrix - chemistry</subject><subject>Gelatin - chemistry</subject><subject>hyaluronic acid</subject><subject>Hyaluronic Acid - chemistry</subject><subject>hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>Proteins</subject><subject>Rheology</subject><subject>Shear Strength</subject><subject>Starch and polysaccharides</subject><subject>Stress, Mechanical</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. 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Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. 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Biosci</addtitle><date>2009-01-09</date><risdate>2009</risdate><volume>9</volume><issue>1</issue><spage>20</spage><epage>28</epage><pages>20-28</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>Hydrogels that mimic the natural extracellular matrix (ECM) are used in three‐dimensional cell culture, cell therapy, and tissue engineering. A semi‐synthetic ECM based on cross‐linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross‐linking density were the main determinants of gel stiffness. 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| subjects | Applied sciences Biocompatible Materials - chemistry Biological and medical sciences biomaterials Cross-Linking Reagents - chemistry Elasticity Exact sciences and technology Extracellular Matrix - chemistry Gelatin - chemistry hyaluronic acid Hyaluronic Acid - chemistry hydrogels Hydrogels - chemistry Materials Testing Medical sciences Molecular Structure Natural polymers Physicochemistry of polymers Proteins Rheology Shear Strength Starch and polysaccharides Stress, Mechanical Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments tissue engineering Tissue Engineering - methods |
| title | Rheological Properties of Cross-Linked Hyaluronan-Gelatin Hydrogels for Tissue Engineering |
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