Phase-State Dependent Current Fluctuations in Pure Lipid Membranes
Current fluctuations in pure lipid membranes have been shown to occur under the influence of transmembrane electric fields (electroporation) as well as a result from structural rearrangements of the lipid bilayer during phase transition (soft perforation). We demonstrate that the ion permeability du...
Gespeichert in:
| Veröffentlicht in: | Biophysical journal 2009-06, Vol.96 (11), p.4592-4597 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4597 |
|---|---|
| container_issue | 11 |
| container_start_page | 4592 |
| container_title | Biophysical journal |
| container_volume | 96 |
| creator | Wunderlich, B. Leirer, C. Idzko, A.-L. Keyser, U.F. Wixforth, A. Myles, V.M. Heimburg, T. Schneider, M.F. |
| description | Current fluctuations in pure lipid membranes have been shown to occur under the influence of transmembrane electric fields (electroporation) as well as a result from structural rearrangements of the lipid bilayer during phase transition (soft perforation). We demonstrate that the ion permeability during lipid phase transition exhibits the same qualitative temperature dependence as the macroscopic heat capacity of a D15PC/DOPC vesicle suspension. Microscopic current fluctuations show distinct characteristics for each individual phase state. Although current fluctuations in the fluid phase show spikelike behavior of short timescales (∼2 ms) with a narrow amplitude distribution, the current fluctuations during lipid phase transition appear in distinct steps with timescales of ∼20 ms. We propose a theoretical explanation for the origin of timescales and permeability based on a linear relationship between lipid membrane susceptibilities and relaxation times near the phase transition. |
| doi_str_mv | 10.1016/j.bpj.2009.02.053 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2711493</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006349509006638</els_id><sourcerecordid>67320194</sourcerecordid><originalsourceid>FETCH-LOGICAL-c476t-6fcd4b395085ffe0404fefa9c344ba0ccef9e649119cf8ba0ba966cfa0a4eb8e3</originalsourceid><addsrcrecordid>eNp9kV-L1DAUxYO4OOOuH8AXKT7sW-tNmmYaBMEdHV0YcWH1OaTpjZPSSWvSDuy3N8MM_nvYpwvJ7x7uOYeQlxQKClS86Ypm7AoGIAtgBVTlE7KkFWc5QC2ekiUAiLzkslqQ5zF2AJRVQJ-RBZW8FqKmS3Jzt9MR8_tJT5h9wBF9i37K1nMIx7npZzPNenKDj5nz2d0cMNu60bXZF9w3QXuMV-TC6j7ii_O8JN83H7-tP-fbr59u1--3ueErMeXCmpY3paygrqxF4MAtWi1NyXmjwRi0EgWXlEpj6_TSaCmEsRo0x6bG8pK8O-mOc7PH1qT7gu7VGNxehwc1aKf-_fFup34MB8VWlHJZJoHrs0AYfs4YJ7V30WDfJxfDHJVYlQxSNAl8_R_YDXPwyZxitBIypVcliJ4gE4YYA9rfl1BQx3pUp1I96liPAqZSPWnn1d8W_myc-0jA2xOAKciDw6CicegNti6gmVQ7uEfkfwE6kKHD</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>215699485</pqid></control><display><type>article</type><title>Phase-State Dependent Current Fluctuations in Pure Lipid Membranes</title><source>MEDLINE</source><source>Cell Press Free Archives</source><source>ScienceDirect Journals (5 years ago - present)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Wunderlich, B. ; Leirer, C. ; Idzko, A.-L. ; Keyser, U.F. ; Wixforth, A. ; Myles, V.M. ; Heimburg, T. ; Schneider, M.F.</creator><creatorcontrib>Wunderlich, B. ; Leirer, C. ; Idzko, A.-L. ; Keyser, U.F. ; Wixforth, A. ; Myles, V.M. ; Heimburg, T. ; Schneider, M.F.</creatorcontrib><description>Current fluctuations in pure lipid membranes have been shown to occur under the influence of transmembrane electric fields (electroporation) as well as a result from structural rearrangements of the lipid bilayer during phase transition (soft perforation). We demonstrate that the ion permeability during lipid phase transition exhibits the same qualitative temperature dependence as the macroscopic heat capacity of a D15PC/DOPC vesicle suspension. Microscopic current fluctuations show distinct characteristics for each individual phase state. Although current fluctuations in the fluid phase show spikelike behavior of short timescales (∼2 ms) with a narrow amplitude distribution, the current fluctuations during lipid phase transition appear in distinct steps with timescales of ∼20 ms. We propose a theoretical explanation for the origin of timescales and permeability based on a linear relationship between lipid membrane susceptibilities and relaxation times near the phase transition.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/j.bpj.2009.02.053</identifier><identifier>PMID: 19486681</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Membrane Permeability ; Electric fields ; Ions ; Ions - chemistry ; Lipid Bilayers - chemistry ; Lipids ; Membrane ; Membrane Potentials ; Membranes ; Models, Chemical ; Phase Transition ; Phase transitions ; Phosphatidylcholines - chemistry ; Temperature ; Thermodynamics ; Time Factors</subject><ispartof>Biophysical journal, 2009-06, Vol.96 (11), p.4592-4597</ispartof><rights>2009 Biophysical Society</rights><rights>Copyright Biophysical Society Jun 3, 2009</rights><rights>2009 by the Biophysical Society. 2009 Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-6fcd4b395085ffe0404fefa9c344ba0ccef9e649119cf8ba0ba966cfa0a4eb8e3</citedby><cites>FETCH-LOGICAL-c476t-6fcd4b395085ffe0404fefa9c344ba0ccef9e649119cf8ba0ba966cfa0a4eb8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711493/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bpj.2009.02.053$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3548,27923,27924,45994,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19486681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wunderlich, B.</creatorcontrib><creatorcontrib>Leirer, C.</creatorcontrib><creatorcontrib>Idzko, A.-L.</creatorcontrib><creatorcontrib>Keyser, U.F.</creatorcontrib><creatorcontrib>Wixforth, A.</creatorcontrib><creatorcontrib>Myles, V.M.</creatorcontrib><creatorcontrib>Heimburg, T.</creatorcontrib><creatorcontrib>Schneider, M.F.</creatorcontrib><title>Phase-State Dependent Current Fluctuations in Pure Lipid Membranes</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>Current fluctuations in pure lipid membranes have been shown to occur under the influence of transmembrane electric fields (electroporation) as well as a result from structural rearrangements of the lipid bilayer during phase transition (soft perforation). We demonstrate that the ion permeability during lipid phase transition exhibits the same qualitative temperature dependence as the macroscopic heat capacity of a D15PC/DOPC vesicle suspension. Microscopic current fluctuations show distinct characteristics for each individual phase state. Although current fluctuations in the fluid phase show spikelike behavior of short timescales (∼2 ms) with a narrow amplitude distribution, the current fluctuations during lipid phase transition appear in distinct steps with timescales of ∼20 ms. We propose a theoretical explanation for the origin of timescales and permeability based on a linear relationship between lipid membrane susceptibilities and relaxation times near the phase transition.</description><subject>Cell Membrane Permeability</subject><subject>Electric fields</subject><subject>Ions</subject><subject>Ions - chemistry</subject><subject>Lipid Bilayers - chemistry</subject><subject>Lipids</subject><subject>Membrane</subject><subject>Membrane Potentials</subject><subject>Membranes</subject><subject>Models, Chemical</subject><subject>Phase Transition</subject><subject>Phase transitions</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Temperature</subject><subject>Thermodynamics</subject><subject>Time Factors</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV-L1DAUxYO4OOOuH8AXKT7sW-tNmmYaBMEdHV0YcWH1OaTpjZPSSWvSDuy3N8MM_nvYpwvJ7x7uOYeQlxQKClS86Ypm7AoGIAtgBVTlE7KkFWc5QC2ekiUAiLzkslqQ5zF2AJRVQJ-RBZW8FqKmS3Jzt9MR8_tJT5h9wBF9i37K1nMIx7npZzPNenKDj5nz2d0cMNu60bXZF9w3QXuMV-TC6j7ii_O8JN83H7-tP-fbr59u1--3ueErMeXCmpY3paygrqxF4MAtWi1NyXmjwRi0EgWXlEpj6_TSaCmEsRo0x6bG8pK8O-mOc7PH1qT7gu7VGNxehwc1aKf-_fFup34MB8VWlHJZJoHrs0AYfs4YJ7V30WDfJxfDHJVYlQxSNAl8_R_YDXPwyZxitBIypVcliJ4gE4YYA9rfl1BQx3pUp1I96liPAqZSPWnn1d8W_myc-0jA2xOAKciDw6CicegNti6gmVQ7uEfkfwE6kKHD</recordid><startdate>20090603</startdate><enddate>20090603</enddate><creator>Wunderlich, B.</creator><creator>Leirer, C.</creator><creator>Idzko, A.-L.</creator><creator>Keyser, U.F.</creator><creator>Wixforth, A.</creator><creator>Myles, V.M.</creator><creator>Heimburg, T.</creator><creator>Schneider, M.F.</creator><general>Elsevier Inc</general><general>Biophysical Society</general><general>The Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090603</creationdate><title>Phase-State Dependent Current Fluctuations in Pure Lipid Membranes</title><author>Wunderlich, B. ; Leirer, C. ; Idzko, A.-L. ; Keyser, U.F. ; Wixforth, A. ; Myles, V.M. ; Heimburg, T. ; Schneider, M.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-6fcd4b395085ffe0404fefa9c344ba0ccef9e649119cf8ba0ba966cfa0a4eb8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Cell Membrane Permeability</topic><topic>Electric fields</topic><topic>Ions</topic><topic>Ions - chemistry</topic><topic>Lipid Bilayers - chemistry</topic><topic>Lipids</topic><topic>Membrane</topic><topic>Membrane Potentials</topic><topic>Membranes</topic><topic>Models, Chemical</topic><topic>Phase Transition</topic><topic>Phase transitions</topic><topic>Phosphatidylcholines - chemistry</topic><topic>Temperature</topic><topic>Thermodynamics</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wunderlich, B.</creatorcontrib><creatorcontrib>Leirer, C.</creatorcontrib><creatorcontrib>Idzko, A.-L.</creatorcontrib><creatorcontrib>Keyser, U.F.</creatorcontrib><creatorcontrib>Wixforth, A.</creatorcontrib><creatorcontrib>Myles, V.M.</creatorcontrib><creatorcontrib>Heimburg, T.</creatorcontrib><creatorcontrib>Schneider, M.F.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wunderlich, B.</au><au>Leirer, C.</au><au>Idzko, A.-L.</au><au>Keyser, U.F.</au><au>Wixforth, A.</au><au>Myles, V.M.</au><au>Heimburg, T.</au><au>Schneider, M.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phase-State Dependent Current Fluctuations in Pure Lipid Membranes</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2009-06-03</date><risdate>2009</risdate><volume>96</volume><issue>11</issue><spage>4592</spage><epage>4597</epage><pages>4592-4597</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>Current fluctuations in pure lipid membranes have been shown to occur under the influence of transmembrane electric fields (electroporation) as well as a result from structural rearrangements of the lipid bilayer during phase transition (soft perforation). We demonstrate that the ion permeability during lipid phase transition exhibits the same qualitative temperature dependence as the macroscopic heat capacity of a D15PC/DOPC vesicle suspension. Microscopic current fluctuations show distinct characteristics for each individual phase state. Although current fluctuations in the fluid phase show spikelike behavior of short timescales (∼2 ms) with a narrow amplitude distribution, the current fluctuations during lipid phase transition appear in distinct steps with timescales of ∼20 ms. We propose a theoretical explanation for the origin of timescales and permeability based on a linear relationship between lipid membrane susceptibilities and relaxation times near the phase transition.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19486681</pmid><doi>10.1016/j.bpj.2009.02.053</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3495 |
| ispartof | Biophysical journal, 2009-06, Vol.96 (11), p.4592-4597 |
| issn | 0006-3495 1542-0086 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2711493 |
| source | MEDLINE; Cell Press Free Archives; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cell Membrane Permeability Electric fields Ions Ions - chemistry Lipid Bilayers - chemistry Lipids Membrane Membrane Potentials Membranes Models, Chemical Phase Transition Phase transitions Phosphatidylcholines - chemistry Temperature Thermodynamics Time Factors |
| title | Phase-State Dependent Current Fluctuations in Pure Lipid Membranes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T06%3A38%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phase-State%20Dependent%20Current%20Fluctuations%20in%20Pure%20Lipid%20Membranes&rft.jtitle=Biophysical%20journal&rft.au=Wunderlich,%20B.&rft.date=2009-06-03&rft.volume=96&rft.issue=11&rft.spage=4592&rft.epage=4597&rft.pages=4592-4597&rft.issn=0006-3495&rft.eissn=1542-0086&rft_id=info:doi/10.1016/j.bpj.2009.02.053&rft_dat=%3Cproquest_pubme%3E67320194%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=215699485&rft_id=info:pmid/19486681&rft_els_id=S0006349509006638&rfr_iscdi=true |