Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: An application for hormone profiling in Oryza sativa
We have developed a highly sensitive and high-throughput method for the simultaneous analysis of 43 molecular species of cytokinins, auxins, ABA and gibberellins. This method consists of an automatic liquid handling system for solid phase extraction and ultra-performance liquid chromatography (UPLC)...
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| Veröffentlicht in: | Plant and cell physiology 2009-07, Vol.50 (7), p.1201-1214 |
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| creator | Kojima, M.(RIKEN, Yokohama (Japan). Plant Science Center) Kamada Nobusada, T Komatsu, H Takei, K Kuroha, T Mizutani, M Ashikari, M Ueguchi Tanaka, M Matsuoka, M Suzuki, K Sakakibara, H |
| description | We have developed a highly sensitive and high-throughput method for the simultaneous analysis of 43 molecular species of cytokinins, auxins, ABA and gibberellins. This method consists of an automatic liquid handling system for solid phase extraction and ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI; UPLC-ESI-qMS/MS). In order to improve the detection limit of negatively charged compounds, such as gibberellins, we chemically derivatized fractions containing auxin, ABA and gibberellins with bromocholine that has a quaternary ammonium functional group. This modification, that we call 'MS-probe', makes these hormone derivatives have a positive ion charge and permits all compounds to be measured in the positive ion mode with UPLC-ESI-qMS/MS in a single run. Consequently, quantification limits of gibberellins increased up to 50-fold. Our current method needs100 mg (FW) of plant tissues to determine phytohormone profiles and enables us to analyze 180 plant samples simultaneously. Application of this method to plant hormone profiling enabled us to draw organ distribution maps of hormone species in rice and also to identify interactions among the four major hormones in the rice gibberellin signaling mutants, gid1-3, gid2-1 and slr1. Combining the results of hormone profiling data with transcriptome data in the gibberellin signaling mutants allows us to analyze relationships between changes in gene expression and hormone metabolism. |
| doi_str_mv | 10.1093/pcp/pcp057 |
| format | Article |
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Plant Science Center) ; Kamada Nobusada, T ; Komatsu, H ; Takei, K ; Kuroha, T ; Mizutani, M ; Ashikari, M ; Ueguchi Tanaka, M ; Matsuoka, M ; Suzuki, K ; Sakakibara, H</creator><creatorcontrib>Kojima, M.(RIKEN, Yokohama (Japan). Plant Science Center) ; Kamada Nobusada, T ; Komatsu, H ; Takei, K ; Kuroha, T ; Mizutani, M ; Ashikari, M ; Ueguchi Tanaka, M ; Matsuoka, M ; Suzuki, K ; Sakakibara, H</creatorcontrib><description>We have developed a highly sensitive and high-throughput method for the simultaneous analysis of 43 molecular species of cytokinins, auxins, ABA and gibberellins. This method consists of an automatic liquid handling system for solid phase extraction and ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI; UPLC-ESI-qMS/MS). In order to improve the detection limit of negatively charged compounds, such as gibberellins, we chemically derivatized fractions containing auxin, ABA and gibberellins with bromocholine that has a quaternary ammonium functional group. This modification, that we call 'MS-probe', makes these hormone derivatives have a positive ion charge and permits all compounds to be measured in the positive ion mode with UPLC-ESI-qMS/MS in a single run. Consequently, quantification limits of gibberellins increased up to 50-fold. Our current method needs100 mg (FW) of plant tissues to determine phytohormone profiles and enables us to analyze 180 plant samples simultaneously. Application of this method to plant hormone profiling enabled us to draw organ distribution maps of hormone species in rice and also to identify interactions among the four major hormones in the rice gibberellin signaling mutants, gid1-3, gid2-1 and slr1. Combining the results of hormone profiling data with transcriptome data in the gibberellin signaling mutants allows us to analyze relationships between changes in gene expression and hormone metabolism.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcp057</identifier><identifier>PMID: 19369275</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>ABA ; Abscisic Acid - analysis ; ACIDE GIBBERELLIQUE ; ACIDO GIBERELICO ; AUXINAS ; AUXINE ; AUXINS ; Chromatography, High Pressure Liquid ; CITOQUININAS ; CYTOKININE ; CYTOKININS ; Cytokinins - analysis ; ESPECTROMETRIA DE MASAS ; Freshwater ; Gene Expression Profiling ; GIBBERELLIC ACID ; Gibberellins ; Gibberellins - analysis ; Indoleacetic Acids - analysis ; MASS SPECTROMETRY ; Oryza - chemistry ; ORYZA SATIVA ; Plant Growth Regulators - analysis ; Special Issue – Regular Papers ; SPECTROMETRIE DE MASSE ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry - methods</subject><ispartof>Plant and cell physiology, 2009-07, Vol.50 (7), p.1201-1214</ispartof><rights>The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5027-aecf6eac94bd3a36eca264efca7c7b414c8d8a1349f774418272f1e60f9e5bc73</citedby><cites>FETCH-LOGICAL-c5027-aecf6eac94bd3a36eca264efca7c7b414c8d8a1349f774418272f1e60f9e5bc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,1578,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19369275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kojima, M.(RIKEN, Yokohama (Japan). Plant Science Center)</creatorcontrib><creatorcontrib>Kamada Nobusada, T</creatorcontrib><creatorcontrib>Komatsu, H</creatorcontrib><creatorcontrib>Takei, K</creatorcontrib><creatorcontrib>Kuroha, T</creatorcontrib><creatorcontrib>Mizutani, M</creatorcontrib><creatorcontrib>Ashikari, M</creatorcontrib><creatorcontrib>Ueguchi Tanaka, M</creatorcontrib><creatorcontrib>Matsuoka, M</creatorcontrib><creatorcontrib>Suzuki, K</creatorcontrib><creatorcontrib>Sakakibara, H</creatorcontrib><title>Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: An application for hormone profiling in Oryza sativa</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>We have developed a highly sensitive and high-throughput method for the simultaneous analysis of 43 molecular species of cytokinins, auxins, ABA and gibberellins. This method consists of an automatic liquid handling system for solid phase extraction and ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI; UPLC-ESI-qMS/MS). In order to improve the detection limit of negatively charged compounds, such as gibberellins, we chemically derivatized fractions containing auxin, ABA and gibberellins with bromocholine that has a quaternary ammonium functional group. This modification, that we call 'MS-probe', makes these hormone derivatives have a positive ion charge and permits all compounds to be measured in the positive ion mode with UPLC-ESI-qMS/MS in a single run. Consequently, quantification limits of gibberellins increased up to 50-fold. Our current method needs100 mg (FW) of plant tissues to determine phytohormone profiles and enables us to analyze 180 plant samples simultaneously. Application of this method to plant hormone profiling enabled us to draw organ distribution maps of hormone species in rice and also to identify interactions among the four major hormones in the rice gibberellin signaling mutants, gid1-3, gid2-1 and slr1. Combining the results of hormone profiling data with transcriptome data in the gibberellin signaling mutants allows us to analyze relationships between changes in gene expression and hormone metabolism.</description><subject>ABA</subject><subject>Abscisic Acid - analysis</subject><subject>ACIDE GIBBERELLIQUE</subject><subject>ACIDO GIBERELICO</subject><subject>AUXINAS</subject><subject>AUXINE</subject><subject>AUXINS</subject><subject>Chromatography, High Pressure Liquid</subject><subject>CITOQUININAS</subject><subject>CYTOKININE</subject><subject>CYTOKININS</subject><subject>Cytokinins - analysis</subject><subject>ESPECTROMETRIA DE MASAS</subject><subject>Freshwater</subject><subject>Gene Expression Profiling</subject><subject>GIBBERELLIC ACID</subject><subject>Gibberellins</subject><subject>Gibberellins - analysis</subject><subject>Indoleacetic Acids - analysis</subject><subject>MASS SPECTROMETRY</subject><subject>Oryza - chemistry</subject><subject>ORYZA SATIVA</subject><subject>Plant Growth Regulators - analysis</subject><subject>Special Issue – Regular Papers</subject><subject>SPECTROMETRIE DE MASSE</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2P1SAUhhujca6jG_caYqILkyqUthQXJpMb9WrGjJnRZOKGcCm0jG1hgN5Yf58_TGrvjB8LXRDIOQ_vOQfeJLmP4DMEKX5uhZ0XLMiNZIVyglIKC3wzWUGIsxSSCh0kd7y_gDCeMbydHCCKS5qRYpV83-im7Sbg5eB10DsJ-FCDNgbT0DozNq0dQ4zxbvLaA6OA7fgQQGtcbwbpwej10ID3Z6l1ZitBb2qttOBBm-GnVKcvR10DEcV6HkzjuG2nNMSU7EHPvQfeShFiVgY3vQBH8Zq13ZWEMu6qFogVlO7mcnoAJ276xoGP1I7fTW4p3nl5b78fJp9ev_q43qTHJ2_ero-OU1HAjKRcClVKLmi-rTHHpRQ8K3OpBCeCbHOUi6quOMI5VYTkOaoykikkS6ioLLaC4MPk5aJrx20vayGH4HjHrNM9dxMzXLM_M4NuWWN2LCOQFvks8GQv4MzlKH1gvfZCdvFJpRk9K0leYYqz_4IZJKSM_UXw0V_ghRld_K6ZQUVZUDirPV0g4Yz3TqrrlhFks4VYtA9bLBThh78P-QvdeyYCjxfAjPbfQunCaR_k12uSuy9xTkwKtjn_zE5zer6uzhA7jfyDhVfcMN447dm7DxmEFMK8QgX-AWiM7rw</recordid><startdate>200907</startdate><enddate>200907</enddate><creator>Kojima, M.(RIKEN, Yokohama (Japan). Plant Science Center)</creator><creator>Kamada Nobusada, T</creator><creator>Komatsu, H</creator><creator>Takei, K</creator><creator>Kuroha, T</creator><creator>Mizutani, M</creator><creator>Ashikari, M</creator><creator>Ueguchi Tanaka, M</creator><creator>Matsuoka, M</creator><creator>Suzuki, K</creator><creator>Sakakibara, H</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200907</creationdate><title>Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: An application for hormone profiling in Oryza sativa</title><author>Kojima, M.(RIKEN, Yokohama (Japan). 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Plant Science Center)</au><au>Kamada Nobusada, T</au><au>Komatsu, H</au><au>Takei, K</au><au>Kuroha, T</au><au>Mizutani, M</au><au>Ashikari, M</au><au>Ueguchi Tanaka, M</au><au>Matsuoka, M</au><au>Suzuki, K</au><au>Sakakibara, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: An application for hormone profiling in Oryza sativa</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2009-07</date><risdate>2009</risdate><volume>50</volume><issue>7</issue><spage>1201</spage><epage>1214</epage><pages>1201-1214</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>We have developed a highly sensitive and high-throughput method for the simultaneous analysis of 43 molecular species of cytokinins, auxins, ABA and gibberellins. This method consists of an automatic liquid handling system for solid phase extraction and ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI; UPLC-ESI-qMS/MS). In order to improve the detection limit of negatively charged compounds, such as gibberellins, we chemically derivatized fractions containing auxin, ABA and gibberellins with bromocholine that has a quaternary ammonium functional group. This modification, that we call 'MS-probe', makes these hormone derivatives have a positive ion charge and permits all compounds to be measured in the positive ion mode with UPLC-ESI-qMS/MS in a single run. Consequently, quantification limits of gibberellins increased up to 50-fold. Our current method needs100 mg (FW) of plant tissues to determine phytohormone profiles and enables us to analyze 180 plant samples simultaneously. Application of this method to plant hormone profiling enabled us to draw organ distribution maps of hormone species in rice and also to identify interactions among the four major hormones in the rice gibberellin signaling mutants, gid1-3, gid2-1 and slr1. Combining the results of hormone profiling data with transcriptome data in the gibberellin signaling mutants allows us to analyze relationships between changes in gene expression and hormone metabolism.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>19369275</pmid><doi>10.1093/pcp/pcp057</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant and cell physiology, 2009-07, Vol.50 (7), p.1201-1214 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | ABA Abscisic Acid - analysis ACIDE GIBBERELLIQUE ACIDO GIBERELICO AUXINAS AUXINE AUXINS Chromatography, High Pressure Liquid CITOQUININAS CYTOKININE CYTOKININS Cytokinins - analysis ESPECTROMETRIA DE MASAS Freshwater Gene Expression Profiling GIBBERELLIC ACID Gibberellins Gibberellins - analysis Indoleacetic Acids - analysis MASS SPECTROMETRY Oryza - chemistry ORYZA SATIVA Plant Growth Regulators - analysis Special Issue – Regular Papers SPECTROMETRIE DE MASSE Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry - methods |
| title | Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: An application for hormone profiling in Oryza sativa |
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