Genetic selection system for improving recombinant membrane protein expression in E. coli

A major barrier to the physical characterization and structure determination of membrane proteins is low yield in recombinant expression. To address this problem, we have designed a selection strategy to isolate mutant strains of Escherichia coli that improve the expression of a targeted membrane pr...

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Veröffentlicht in:	Protein science 2009-02, Vol.18 (2), p.372-383
Hauptverfasser:	Massey‐Gendel, Elizabeth, Zhao, Anni, Boulting, Gabriella, Kim, Hye‐Yeon, Balamotis, Michael A., Seligman, Len M., Nakamoto, Robert K., Bowie, James U.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Cloning, Molecular DNA Restriction Enzymes - metabolism E. coli Electrophoresis, Polyacrylamide Gel Escherichia coli - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Mutation Mycobacterium tuberculosis - genetics plasmid curing Plasmids - genetics Plasmids - metabolism recombinant membrane protein expression Recombinant Proteins - genetics Recombinant Proteins - metabolism
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