Spectrum of mitochondrial DNA deletions within the common deletion region induced by low levels of UVB irradiation of human keratinocytes in vitro

We show that a single low-dose exposure of human epidermal keratinocytes (NHEK) to an FS20 light source in vitro can induce the formation of mitochondrial DNA deletions in a PCR detection assay. We used primer sets specifically designed to exclude amplification of segments containing the common dele...

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Veröffentlicht in:	Gene 2009-07, Vol.440 (1), p.23-27
Hauptverfasser:	Hwang, Bor-Jang, Kuttamperoor, Francis, Wu, Julia, Steinberg, Mark L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence - radiation effects DNA Damage DNA, Mitochondrial - chemistry Humans Inverted repeats Keratinocytes Keratinocytes - radiation effects Models, Genetic Molecular Sequence Data mtDNA deletion Reactive Oxygen Species - metabolism Sequence Deletion Ultraviolet Rays - adverse effects UVB
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