Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc
The study of regeneration would be aided greatly by systems that support large-scale genetic screens. Here we describe a nonsurgical method for inducing tissue damage and regeneration in Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner....
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| Veröffentlicht in: | Developmental cell 2009-06, Vol.16 (6), p.797-809 |
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| creator | Smith-Bolton, Rachel K. Worley, Melanie I. Kanda, Hiroshi Hariharan, Iswar K. |
| description | The study of regeneration would be aided greatly by systems that support large-scale genetic screens. Here we describe a nonsurgical method for inducing tissue damage and regeneration in
Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner. Tissue damage results in localized regenerative proliferation characterized by altered expression of patterning genes and growth regulators as well as a temporary loss of markers of cell fate commitment. Wingless and Myc are induced by tissue damage and are important for regenerative growth. Furthermore, ectopic Myc enhances regeneration when other growth drivers tested do not. As the animal matures, the ability to regenerate is lost and cannot be restored by activation of Wingless or Myc. This system is conducive to forward genetic screens, enabling an unbiased search for genes that regulate both the extent of and the capacity for regeneration. |
| doi_str_mv | 10.1016/j.devcel.2009.04.015 |
| format | Article |
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Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner. Tissue damage results in localized regenerative proliferation characterized by altered expression of patterning genes and growth regulators as well as a temporary loss of markers of cell fate commitment. Wingless and Myc are induced by tissue damage and are important for regenerative growth. Furthermore, ectopic Myc enhances regeneration when other growth drivers tested do not. As the animal matures, the ability to regenerate is lost and cannot be restored by activation of Wingless or Myc. This system is conducive to forward genetic screens, enabling an unbiased search for genes that regulate both the extent of and the capacity for regeneration.</description><identifier>ISSN: 1534-5807</identifier><identifier>EISSN: 1878-1551</identifier><identifier>DOI: 10.1016/j.devcel.2009.04.015</identifier><identifier>PMID: 19531351</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Body Patterning ; Cell differentiation, maturation, development, hematopoiesis ; Cell Lineage ; Cell physiology ; Cell Proliferation ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cyclin E - metabolism ; DEVBIO ; DNA-Binding Proteins - metabolism ; Drosophila melanogaster - cytology ; Drosophila melanogaster - genetics ; Drosophila melanogaster - growth & development ; Drosophila melanogaster - metabolism ; Drosophila Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Genes, Insect ; Intercellular Signaling Peptides and Proteins - metabolism ; Larva - cytology ; Larva - growth & development ; Molecular and cellular biology ; Mutation - genetics ; Regeneration ; Transcription Factors - metabolism ; Up-Regulation - genetics ; Wings, Animal - cytology ; Wings, Animal - growth & development ; Wings, Animal - metabolism ; Wnt1 Protein - metabolism</subject><ispartof>Developmental cell, 2009-06, Vol.16 (6), p.797-809</ispartof><rights>2009 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><rights>2009 Elsevier Inc. All rights reserved. 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-44e481ca9058ab1e7feb66747feb85eade626e31ea68392aa2a2412e96b6625d3</citedby><cites>FETCH-LOGICAL-c491t-44e481ca9058ab1e7feb66747feb85eade626e31ea68392aa2a2412e96b6625d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.devcel.2009.04.015$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21640090$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19531351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith-Bolton, Rachel K.</creatorcontrib><creatorcontrib>Worley, Melanie I.</creatorcontrib><creatorcontrib>Kanda, Hiroshi</creatorcontrib><creatorcontrib>Hariharan, Iswar K.</creatorcontrib><title>Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc</title><title>Developmental cell</title><addtitle>Dev Cell</addtitle><description>The study of regeneration would be aided greatly by systems that support large-scale genetic screens. Here we describe a nonsurgical method for inducing tissue damage and regeneration in
Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner. Tissue damage results in localized regenerative proliferation characterized by altered expression of patterning genes and growth regulators as well as a temporary loss of markers of cell fate commitment. Wingless and Myc are induced by tissue damage and are important for regenerative growth. Furthermore, ectopic Myc enhances regeneration when other growth drivers tested do not. As the animal matures, the ability to regenerate is lost and cannot be restored by activation of Wingless or Myc. This system is conducive to forward genetic screens, enabling an unbiased search for genes that regulate both the extent of and the capacity for regeneration.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Patterning</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell Lineage</subject><subject>Cell physiology</subject><subject>Cell Proliferation</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cyclin E - metabolism</subject><subject>DEVBIO</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Drosophila melanogaster - cytology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - growth & development</subject><subject>Drosophila melanogaster - metabolism</subject><subject>Drosophila Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Insect</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Larva - cytology</subject><subject>Larva - growth & development</subject><subject>Molecular and cellular biology</subject><subject>Mutation - genetics</subject><subject>Regeneration</subject><subject>Transcription Factors - metabolism</subject><subject>Up-Regulation - genetics</subject><subject>Wings, Animal - cytology</subject><subject>Wings, Animal - growth & development</subject><subject>Wings, Animal - metabolism</subject><subject>Wnt1 Protein - metabolism</subject><issn>1534-5807</issn><issn>1878-1551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFvEzEQhS0EoqXwDxDyBW67eHZtr_eChFookYoQCMTRmngniSPHG-xNUP49jhK1cOE0I_l7zzPzGHsJogYB-u26HmjvKNSNEH0tZC1APWKXYDpTgVLwuPSqlZUyortgz3JeiyIDI56yC-hVC62CS_b1Gy0pUsLJ74nfpvH3tOI-8ps05nG78gH5bINLHzHwG59d5rPMi2YXcKKBzw_8p4_LQDlzjAP_fHDP2ZMFhkwvzvWK_fj44fv1p-ruy-3s-v1d5WQPUyUlSQMOe6EMzoG6Bc217uSxGkU4kG40tUCoTds3iA02EhrqdcEaNbRX7N3Jd7ubb2hwFKeEwW6T32A62BG9_fcl-pVdjnvbdEJBB8Xgzdkgjb92lCe7KQtSCBhp3GWru9Z0nWwLKE-gK0fJiRb3n4Cwxyzs2p6ysMcsrJC2ZFFkr_4e8EF0Pn4BXp8BzA7DImF0Pt9zDWhZ7MTDplTOufeUbHaeoqPBJ3KTHUb__0n-AOpHqns</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Smith-Bolton, Rachel K.</creator><creator>Worley, Melanie I.</creator><creator>Kanda, Hiroshi</creator><creator>Hariharan, Iswar K.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090601</creationdate><title>Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc</title><author>Smith-Bolton, Rachel K. ; Worley, Melanie I. ; Kanda, Hiroshi ; Hariharan, Iswar K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-44e481ca9058ab1e7feb66747feb85eade626e31ea68392aa2a2412e96b6625d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Patterning</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell Lineage</topic><topic>Cell physiology</topic><topic>Cell Proliferation</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cyclin E - metabolism</topic><topic>DEVBIO</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Drosophila melanogaster - cytology</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila melanogaster - metabolism</topic><topic>Drosophila Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Insect</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Larva - cytology</topic><topic>Larva - growth & development</topic><topic>Molecular and cellular biology</topic><topic>Mutation - genetics</topic><topic>Regeneration</topic><topic>Transcription Factors - metabolism</topic><topic>Up-Regulation - genetics</topic><topic>Wings, Animal - cytology</topic><topic>Wings, Animal - growth & development</topic><topic>Wings, Animal - metabolism</topic><topic>Wnt1 Protein - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith-Bolton, Rachel K.</creatorcontrib><creatorcontrib>Worley, Melanie I.</creatorcontrib><creatorcontrib>Kanda, Hiroshi</creatorcontrib><creatorcontrib>Hariharan, Iswar K.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Developmental cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith-Bolton, Rachel K.</au><au>Worley, Melanie I.</au><au>Kanda, Hiroshi</au><au>Hariharan, Iswar K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc</atitle><jtitle>Developmental cell</jtitle><addtitle>Dev Cell</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>16</volume><issue>6</issue><spage>797</spage><epage>809</epage><pages>797-809</pages><issn>1534-5807</issn><eissn>1878-1551</eissn><abstract>The study of regeneration would be aided greatly by systems that support large-scale genetic screens. Here we describe a nonsurgical method for inducing tissue damage and regeneration in
Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner. Tissue damage results in localized regenerative proliferation characterized by altered expression of patterning genes and growth regulators as well as a temporary loss of markers of cell fate commitment. Wingless and Myc are induced by tissue damage and are important for regenerative growth. Furthermore, ectopic Myc enhances regeneration when other growth drivers tested do not. As the animal matures, the ability to regenerate is lost and cannot be restored by activation of Wingless or Myc. This system is conducive to forward genetic screens, enabling an unbiased search for genes that regulate both the extent of and the capacity for regeneration.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>19531351</pmid><doi>10.1016/j.devcel.2009.04.015</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Body Patterning Cell differentiation, maturation, development, hematopoiesis Cell Lineage Cell physiology Cell Proliferation Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cyclin E - metabolism DEVBIO DNA-Binding Proteins - metabolism Drosophila melanogaster - cytology Drosophila melanogaster - genetics Drosophila melanogaster - growth & development Drosophila melanogaster - metabolism Drosophila Proteins - metabolism Fundamental and applied biological sciences. Psychology Genes, Insect Intercellular Signaling Peptides and Proteins - metabolism Larva - cytology Larva - growth & development Molecular and cellular biology Mutation - genetics Regeneration Transcription Factors - metabolism Up-Regulation - genetics Wings, Animal - cytology Wings, Animal - growth & development Wings, Animal - metabolism Wnt1 Protein - metabolism |
| title | Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc |
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