RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae
The cellular response to stress conditions involves a decision between survival or cell death when damage is severe. A conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer RNAs (tRNAs). The mechanism and significance of such tRNA cleavage is unknown. We show that in...
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| Veröffentlicht in: | The Journal of cell biology 2009-04, Vol.185 (1), p.43-50 |
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| creator | Thompson, Debrah M Parker, Roy |
| description | The cellular response to stress conditions involves a decision between survival or cell death when damage is severe. A conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer RNAs (tRNAs). The mechanism and significance of such tRNA cleavage is unknown. We show that in yeast, tRNAs are cleaved by the RNase T2 family member Rny1p, which is released from the vacuole into the cytosol during oxidative stress. Rny1p modulates yeast cell survival during oxidative stress independently of its catalytic ability. This suggests that upon release to the cytosol, Rny1p promotes cell death by direct interactions with downstream components. Thus, detection of Rny1p, and possibly its orthologues, in the cytosol may be a conserved mechanism for assessing cellular damage and determining cell survival, analogous to the role of cytochrome c as a marker for mitochondrial damage. |
| doi_str_mv | 10.1083/jcb.200811119 |
| format | Article |
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A conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer RNAs (tRNAs). The mechanism and significance of such tRNA cleavage is unknown. We show that in yeast, tRNAs are cleaved by the RNase T2 family member Rny1p, which is released from the vacuole into the cytosol during oxidative stress. Rny1p modulates yeast cell survival during oxidative stress independently of its catalytic ability. This suggests that upon release to the cytosol, Rny1p promotes cell death by direct interactions with downstream components. 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A conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer RNAs (tRNAs). The mechanism and significance of such tRNA cleavage is unknown. We show that in yeast, tRNAs are cleaved by the RNase T2 family member Rny1p, which is released from the vacuole into the cytosol during oxidative stress. Rny1p modulates yeast cell survival during oxidative stress independently of its catalytic ability. This suggests that upon release to the cytosol, Rny1p promotes cell death by direct interactions with downstream components. Thus, detection of Rny1p, and possibly its orthologues, in the cytosol may be a conserved mechanism for assessing cellular damage and determining cell survival, analogous to the role of cytochrome c as a marker for mitochondrial damage.</description><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Biochemistry</subject><subject>Cell death</subject><subject>Cell lines</subject><subject>Cytosol</subject><subject>Cytosol - enzymology</subject><subject>Cytosol - metabolism</subject><subject>Eukaryotes</subject><subject>Humans</subject><subject>Oxidative Stress</subject><subject>Protein synthesis</subject><subject>Ribonucleases - genetics</subject><subject>Ribonucleases - metabolism</subject><subject>Ribonucleases - physiology</subject><subject>RNA, Transfer - metabolism</subject><subject>Saccharomyces cerevisiae - cytology</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - physiology</subject><subject>Steepest descent method</subject><subject>Transfer RNA</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Tumor Suppressor Proteins - physiology</subject><subject>Tumors</subject><subject>Vacuoles</subject><subject>Vacuoles - enzymology</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2P0zAQxS0EYsvCkSNgceCWZcZ24uSCtFrxJa0WqcueLceZtK7auNhJRf97XFqVD19G8vvN0xs9xl4iXCHU8v3KtVcCoMb8mkdshqWCokYFj9kMQGDRlKK8YM9SWgGA0ko-ZRfYSCnqBmeM5nc2EZ8Pe9xytya7o8TH-d114nbo-DaGTRjzl6P1mndkxyXvpuiHBQ8_fWdHvyOexkgpcT_we-vc0uadvfu9E2nnk7f0nD3p7TrRi9O8ZA-fPn6_-VLcfvv89eb6tnClUGOhUUoiTVUnSbWqdkLJDrESlepUX9YtkLZC9EKpvsa674Rrmh6kLbFFhSQv2Yej73ZqN9Q5GsZo12Yb_cbGvQnWm3-VwS_NIuyM0AAlqmzw7mQQw4-J0mg2Ph1utwOFKZlKo2hqKDP49j9wFaY45OOMQI2gGt1kqDhCLoaUIvXnJAjm0J7J7Zlze5l__Xf8P_Sprgy8OgKrNIZ41kUOpIXQWX9z1HsbjF1En8zDvQCUgBXqA_YLapao9g</recordid><startdate>20090406</startdate><enddate>20090406</enddate><creator>Thompson, Debrah M</creator><creator>Parker, Roy</creator><general>The Rockefeller University Press</general><general>Rockefeller University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090406</creationdate><title>RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae</title><author>Thompson, Debrah M ; Parker, Roy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-7133ee7e6d3e4b48c243d116264d4f58b0e7a22f244f818fd2c99f03a51b141e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Biochemistry</topic><topic>Cell death</topic><topic>Cell lines</topic><topic>Cytosol</topic><topic>Cytosol - enzymology</topic><topic>Cytosol - metabolism</topic><topic>Eukaryotes</topic><topic>Humans</topic><topic>Oxidative Stress</topic><topic>Protein synthesis</topic><topic>Ribonucleases - genetics</topic><topic>Ribonucleases - metabolism</topic><topic>Ribonucleases - physiology</topic><topic>RNA, Transfer - metabolism</topic><topic>Saccharomyces cerevisiae - cytology</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - physiology</topic><topic>Steepest descent method</topic><topic>Transfer RNA</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Tumor Suppressor Proteins - physiology</topic><topic>Tumors</topic><topic>Vacuoles</topic><topic>Vacuoles - enzymology</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thompson, Debrah M</creatorcontrib><creatorcontrib>Parker, Roy</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thompson, Debrah M</au><au>Parker, Roy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>2009-04-06</date><risdate>2009</risdate><volume>185</volume><issue>1</issue><spage>43</spage><epage>50</epage><pages>43-50</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>The cellular response to stress conditions involves a decision between survival or cell death when damage is severe. A conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer RNAs (tRNAs). The mechanism and significance of such tRNA cleavage is unknown. We show that in yeast, tRNAs are cleaved by the RNase T2 family member Rny1p, which is released from the vacuole into the cytosol during oxidative stress. Rny1p modulates yeast cell survival during oxidative stress independently of its catalytic ability. This suggests that upon release to the cytosol, Rny1p promotes cell death by direct interactions with downstream components. Thus, detection of Rny1p, and possibly its orthologues, in the cytosol may be a conserved mechanism for assessing cellular damage and determining cell survival, analogous to the role of cytochrome c as a marker for mitochondrial damage.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>19332891</pmid><doi>10.1083/jcb.200811119</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Apoptosis - physiology Biochemistry Cell death Cell lines Cytosol Cytosol - enzymology Cytosol - metabolism Eukaryotes Humans Oxidative Stress Protein synthesis Ribonucleases - genetics Ribonucleases - metabolism Ribonucleases - physiology RNA, Transfer - metabolism Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - physiology Steepest descent method Transfer RNA Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism Tumor Suppressor Proteins - physiology Tumors Vacuoles Vacuoles - enzymology Yeast Yeasts |
| title | RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae |
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