Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells
The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS ins...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2009-03, Vol.59 (2), p.135-141 |
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| creator | Fujiwara, Masashi Tsukada, Ryohei Shioya, Itaru Takagi, Mutsumi |
| description | The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10 to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56 °C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS. |
| doi_str_mv | 10.1007/s10616-009-9199-7 |
| format | Article |
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The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10 to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56 °C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-009-9199-7</identifier><identifier>PMID: 19484373</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Biochemistry ; Biological and medical sciences ; Biomedicine ; Biotechnology ; Cell adhesion & migration ; Cell culture ; Cell density ; Cell growth ; Chemistry ; Chemistry and Materials Science ; Colony-stimulating factor ; Dihydrofolate reductase ; Fetal calf serum ; Fibroblasts ; Fundamental and applied biological sciences. Psychology ; Granulocyte-macrophage colony-stimulating factor ; Growth factors ; Heat treating ; Heat treatments ; JAACT Special Issue ; Lipids ; Ovaries</subject><ispartof>Cytotechnology (Dordrecht), 2009-03, Vol.59 (2), p.135-141</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><rights>2009 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2009.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c666t-6b3f1103251ce078dd9f921d6b6c9a87c09b1d284989bb64e85c4aeaf985b7433</citedby><cites>FETCH-LOGICAL-c666t-6b3f1103251ce078dd9f921d6b6c9a87c09b1d284989bb64e85c4aeaf985b7433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698441/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918256857?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,27924,27925,33744,33745,41488,42557,43805,51319,53791,53793,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21684129$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19484373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujiwara, Masashi</creatorcontrib><creatorcontrib>Tsukada, Ryohei</creatorcontrib><creatorcontrib>Shioya, Itaru</creatorcontrib><creatorcontrib>Takagi, Mutsumi</creatorcontrib><title>Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10 to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56 °C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS.</description><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cell growth</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colony-stimulating factor</subject><subject>Dihydrofolate reductase</subject><subject>Fetal calf serum</subject><subject>Fibroblasts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulocyte-macrophage colony-stimulating factor</subject><subject>Growth factors</subject><subject>Heat treating</subject><subject>Heat treatments</subject><subject>JAACT Special Issue</subject><subject>Lipids</subject><subject>Ovaries</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kk2P1SAUhonROOPVH-BGSYzRTZUDlMLGxNyMH8kkLnTWhFK47aSFEdoxxj8vtTcz6mI2QDjP-3IO5yD0FMgbIKR5m4EIEBUhqlKgVNXcQ6dQN6wiTSPvo1OiKKkUEeoEPcr5khSwAfYQnYDikrOGnaJfZ947O2ccPe6dmfGcyjq5MGMTOmxjsOWczDzEsDJ-yD3OLi0TLhfWjSM-pPhj7vEQsOl6l1fQLuO8JLcK9v0QXHa4N1OeXcLx2qSff4T5MXrgzZjdk-O-Qxcfzr7tP1XnXz5-3r8_r6wQYq5EyzwAYbQG60gju055RaETrbDKyMYS1UJHJVdSta3gTtaWG2e8knXbcMZ26N3me7W0k-u2gkZ9lYap5KKjGfS_kTD0-hCvNRVKcg7F4NXRIMXvi8uznoa8lmCCi0vWDWOUCsp4IV_fSQIBXlpXi9X0xX_oZVxSKB-hqQJJayFLK3cINsqmmHNy_iZtIHodAr0NgS691esQ6FXz7O96bxXHrhfg5REw2ZrRJxPskG84CkJyoKpwdONyCYWDS7cp3vX6803kTdTmkIrxxVdKgBEQvGaEst8gb9Q5</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Fujiwara, Masashi</creator><creator>Tsukada, Ryohei</creator><creator>Shioya, Itaru</creator><creator>Takagi, Mutsumi</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090301</creationdate><title>Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells</title><author>Fujiwara, Masashi ; Tsukada, Ryohei ; Shioya, Itaru ; Takagi, Mutsumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c666t-6b3f1103251ce078dd9f921d6b6c9a87c09b1d284989bb64e85c4aeaf985b7433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cell density</topic><topic>Cell growth</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colony-stimulating factor</topic><topic>Dihydrofolate reductase</topic><topic>Fetal calf serum</topic><topic>Fibroblasts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulocyte-macrophage colony-stimulating factor</topic><topic>Growth factors</topic><topic>Heat treating</topic><topic>Heat treatments</topic><topic>JAACT Special Issue</topic><topic>Lipids</topic><topic>Ovaries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujiwara, Masashi</creatorcontrib><creatorcontrib>Tsukada, Ryohei</creatorcontrib><creatorcontrib>Shioya, Itaru</creatorcontrib><creatorcontrib>Takagi, Mutsumi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujiwara, Masashi</au><au>Tsukada, Ryohei</au><au>Shioya, Itaru</au><au>Takagi, Mutsumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>59</volume><issue>2</issue><spage>135</spage><epage>141</epage><pages>135-141</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10 to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56 °C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. 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| ispartof | Cytotechnology (Dordrecht), 2009-03, Vol.59 (2), p.135-141 |
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| source | SpringerNature Journals; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Biochemistry Biological and medical sciences Biomedicine Biotechnology Cell adhesion & migration Cell culture Cell density Cell growth Chemistry Chemistry and Materials Science Colony-stimulating factor Dihydrofolate reductase Fetal calf serum Fibroblasts Fundamental and applied biological sciences. Psychology Granulocyte-macrophage colony-stimulating factor Growth factors Heat treating Heat treatments JAACT Special Issue Lipids Ovaries |
| title | Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells |
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