Cyclooxygenase enzyme expression and E series prostaglandin receptor signalling are enhanced in heavy menstruation
BACKGROUND Although the mechanisms underlying the causes of heavy menstrual blood loss (MBL) remain to be elucidated, prostaglandins have been previously implicated. This study was initiated to elucidate a pattern of expression of the various components of the cyclooxygenase (COX)–prostaglandin sign...
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| Veröffentlicht in: | Human reproduction (Oxford) 2007-05, Vol.22 (5), p.1450-1456 |
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| creator | Smith, O.P.Milling Jabbour, H.N. Critchley, H.O.D. |
| description | BACKGROUND
Although the mechanisms underlying the causes of heavy menstrual blood loss (MBL) remain to be elucidated, prostaglandins have been previously implicated. This study was initiated to elucidate a pattern of expression of the various components of the cyclooxygenase (COX)–prostaglandin signalling pathways present in the endometrium of women with normal and heavy MBLs.
METHODS
Endometrial biopsies were collected at different stages of the menstrual cycle from women who underwent measurement of MBL. Tissue was divided for either examination of gene expression by quantitative RT–PCR analysis or in vitro culture experimentation.
RESULTS
Analysis of gene expression demonstrated a significant elevation in expression of COX-1 and COX-2 mRNA in endometrium obtained from women with heavy MBL when compared with endometrium obtained from women with normal MBL. Tissue culture with PGE2 stimulation caused a significantly elevated production of cyclic AMP (cAMP) by endometrium of women with heavy MBL when compared with normal MBL. Expression of phosphodiesterase 4B, an enzyme involved in cAMP breakdown, was reduced in these same endometrial samples obtained from women with heavy MBL.
CONCLUSIONS
These data identify the E series prostaglandin receptors and their signalling pathways as potential therapeutic targets in the treatment of heavy menstruation. |
| doi_str_mv | 10.1093/humrep/del503 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2694303</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/humrep/del503</oup_id><sourcerecordid>1261040311</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-72dbce285741de54550ec841029497340c80a7aee7697dcbcef7a3b8a02d07c43</originalsourceid><addsrcrecordid>eNqFkc1rFTEUxYMo9lldupUgKG6mzdckM5uCPOoHFLrRdcjL3DcvJZOMyUzp-NebMg-r3XR1Q_Lj5NxzEHpLyRklLT8_zEOC8bwDXxP-DG2okKRivCbP0YYw2VSUSnqCXuV8Q0g5NvIlOqGKSUEJ36C0XayP8W7pIZgMGMLvZSjjbkyQs4sBm9DhS5whOch4TDFPpvfl0gWcwMI4xYSz64Px3oUem3QvcjDBQocLcwBzu-ABQp7SbKai-Bq92Buf4c1xnqKfXy5_bL9VV9dfv28_X1VWqHqqFOt2FlhTK0E7qEVdE7BNcc1a0SouiG2IUQZAyVZ1trB7ZfiuMYR1RFnBT9HFqjvOuwE6C2FKxusxucGkRUfj9P8vwR10H281k63ghBeBj0eBFH_NkCc9uGzBl-0hzlkrwtta1qqA7x-BN3FOJZGsGaUtEW3TFqhaIVsyzAn2f51Qou-r1GuVeq2y8O_-tf9AH7srwIcjYLI1fp9K5i4_cI2SJShWuE8rF-fxiT__AC9iupQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>211904989</pqid></control><display><type>article</type><title>Cyclooxygenase enzyme expression and E series prostaglandin receptor signalling are enhanced in heavy menstruation</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Smith, O.P.Milling ; Jabbour, H.N. ; Critchley, H.O.D.</creator><creatorcontrib>Smith, O.P.Milling ; Jabbour, H.N. ; Critchley, H.O.D.</creatorcontrib><description>BACKGROUND
Although the mechanisms underlying the causes of heavy menstrual blood loss (MBL) remain to be elucidated, prostaglandins have been previously implicated. This study was initiated to elucidate a pattern of expression of the various components of the cyclooxygenase (COX)–prostaglandin signalling pathways present in the endometrium of women with normal and heavy MBLs.
METHODS
Endometrial biopsies were collected at different stages of the menstrual cycle from women who underwent measurement of MBL. Tissue was divided for either examination of gene expression by quantitative RT–PCR analysis or in vitro culture experimentation.
RESULTS
Analysis of gene expression demonstrated a significant elevation in expression of COX-1 and COX-2 mRNA in endometrium obtained from women with heavy MBL when compared with endometrium obtained from women with normal MBL. Tissue culture with PGE2 stimulation caused a significantly elevated production of cyclic AMP (cAMP) by endometrium of women with heavy MBL when compared with normal MBL. Expression of phosphodiesterase 4B, an enzyme involved in cAMP breakdown, was reduced in these same endometrial samples obtained from women with heavy MBL.
CONCLUSIONS
These data identify the E series prostaglandin receptors and their signalling pathways as potential therapeutic targets in the treatment of heavy menstruation.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/del503</identifier><identifier>PMID: 17264103</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>3',5'-Cyclic-AMP Phosphodiesterases - physiology ; Adult ; Biological and medical sciences ; Cyclic AMP - biosynthesis ; Cyclic Nucleotide Phosphodiesterases, Type 4 ; Cyclooxygenase 1 - biosynthesis ; Cyclooxygenase 2 - biosynthesis ; Female ; Follicular Phase - physiology ; Gynecology. Andrology. Obstetrics ; Humans ; Luteal Phase - physiology ; Medical sciences ; Menorrhagia - enzymology ; Menorrhagia - physiopathology ; Middle Aged ; Receptors, Prostaglandin E - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction - physiology</subject><ispartof>Human reproduction (Oxford), 2007-05, Vol.22 (5), p.1450-1456</ispartof><rights>The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org 2007</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) May 2007</rights><rights>The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-72dbce285741de54550ec841029497340c80a7aee7697dcbcef7a3b8a02d07c43</citedby><cites>FETCH-LOGICAL-c475t-72dbce285741de54550ec841029497340c80a7aee7697dcbcef7a3b8a02d07c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,1578,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18764972$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17264103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, O.P.Milling</creatorcontrib><creatorcontrib>Jabbour, H.N.</creatorcontrib><creatorcontrib>Critchley, H.O.D.</creatorcontrib><title>Cyclooxygenase enzyme expression and E series prostaglandin receptor signalling are enhanced in heavy menstruation</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>BACKGROUND
Although the mechanisms underlying the causes of heavy menstrual blood loss (MBL) remain to be elucidated, prostaglandins have been previously implicated. This study was initiated to elucidate a pattern of expression of the various components of the cyclooxygenase (COX)–prostaglandin signalling pathways present in the endometrium of women with normal and heavy MBLs.
METHODS
Endometrial biopsies were collected at different stages of the menstrual cycle from women who underwent measurement of MBL. Tissue was divided for either examination of gene expression by quantitative RT–PCR analysis or in vitro culture experimentation.
RESULTS
Analysis of gene expression demonstrated a significant elevation in expression of COX-1 and COX-2 mRNA in endometrium obtained from women with heavy MBL when compared with endometrium obtained from women with normal MBL. Tissue culture with PGE2 stimulation caused a significantly elevated production of cyclic AMP (cAMP) by endometrium of women with heavy MBL when compared with normal MBL. Expression of phosphodiesterase 4B, an enzyme involved in cAMP breakdown, was reduced in these same endometrial samples obtained from women with heavy MBL.
CONCLUSIONS
These data identify the E series prostaglandin receptors and their signalling pathways as potential therapeutic targets in the treatment of heavy menstruation.</description><subject>3',5'-Cyclic-AMP Phosphodiesterases - physiology</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Cyclic Nucleotide Phosphodiesterases, Type 4</subject><subject>Cyclooxygenase 1 - biosynthesis</subject><subject>Cyclooxygenase 2 - biosynthesis</subject><subject>Female</subject><subject>Follicular Phase - physiology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Luteal Phase - physiology</subject><subject>Medical sciences</subject><subject>Menorrhagia - enzymology</subject><subject>Menorrhagia - physiopathology</subject><subject>Middle Aged</subject><subject>Receptors, Prostaglandin E - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Signal Transduction - physiology</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFTEUxYMo9lldupUgKG6mzdckM5uCPOoHFLrRdcjL3DcvJZOMyUzp-NebMg-r3XR1Q_Lj5NxzEHpLyRklLT8_zEOC8bwDXxP-DG2okKRivCbP0YYw2VSUSnqCXuV8Q0g5NvIlOqGKSUEJ36C0XayP8W7pIZgMGMLvZSjjbkyQs4sBm9DhS5whOch4TDFPpvfl0gWcwMI4xYSz64Px3oUem3QvcjDBQocLcwBzu-ABQp7SbKai-Bq92Buf4c1xnqKfXy5_bL9VV9dfv28_X1VWqHqqFOt2FlhTK0E7qEVdE7BNcc1a0SouiG2IUQZAyVZ1trB7ZfiuMYR1RFnBT9HFqjvOuwE6C2FKxusxucGkRUfj9P8vwR10H281k63ghBeBj0eBFH_NkCc9uGzBl-0hzlkrwtta1qqA7x-BN3FOJZGsGaUtEW3TFqhaIVsyzAn2f51Qou-r1GuVeq2y8O_-tf9AH7srwIcjYLI1fp9K5i4_cI2SJShWuE8rF-fxiT__AC9iupQ</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Smith, O.P.Milling</creator><creator>Jabbour, H.N.</creator><creator>Critchley, H.O.D.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070501</creationdate><title>Cyclooxygenase enzyme expression and E series prostaglandin receptor signalling are enhanced in heavy menstruation</title><author>Smith, O.P.Milling ; Jabbour, H.N. ; Critchley, H.O.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-72dbce285741de54550ec841029497340c80a7aee7697dcbcef7a3b8a02d07c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>3',5'-Cyclic-AMP Phosphodiesterases - physiology</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Cyclic Nucleotide Phosphodiesterases, Type 4</topic><topic>Cyclooxygenase 1 - biosynthesis</topic><topic>Cyclooxygenase 2 - biosynthesis</topic><topic>Female</topic><topic>Follicular Phase - physiology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Luteal Phase - physiology</topic><topic>Medical sciences</topic><topic>Menorrhagia - enzymology</topic><topic>Menorrhagia - physiopathology</topic><topic>Middle Aged</topic><topic>Receptors, Prostaglandin E - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Signal Transduction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, O.P.Milling</creatorcontrib><creatorcontrib>Jabbour, H.N.</creatorcontrib><creatorcontrib>Critchley, H.O.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, O.P.Milling</au><au>Jabbour, H.N.</au><au>Critchley, H.O.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclooxygenase enzyme expression and E series prostaglandin receptor signalling are enhanced in heavy menstruation</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>22</volume><issue>5</issue><spage>1450</spage><epage>1456</epage><pages>1450-1456</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND
Although the mechanisms underlying the causes of heavy menstrual blood loss (MBL) remain to be elucidated, prostaglandins have been previously implicated. This study was initiated to elucidate a pattern of expression of the various components of the cyclooxygenase (COX)–prostaglandin signalling pathways present in the endometrium of women with normal and heavy MBLs.
METHODS
Endometrial biopsies were collected at different stages of the menstrual cycle from women who underwent measurement of MBL. Tissue was divided for either examination of gene expression by quantitative RT–PCR analysis or in vitro culture experimentation.
RESULTS
Analysis of gene expression demonstrated a significant elevation in expression of COX-1 and COX-2 mRNA in endometrium obtained from women with heavy MBL when compared with endometrium obtained from women with normal MBL. Tissue culture with PGE2 stimulation caused a significantly elevated production of cyclic AMP (cAMP) by endometrium of women with heavy MBL when compared with normal MBL. Expression of phosphodiesterase 4B, an enzyme involved in cAMP breakdown, was reduced in these same endometrial samples obtained from women with heavy MBL.
CONCLUSIONS
These data identify the E series prostaglandin receptors and their signalling pathways as potential therapeutic targets in the treatment of heavy menstruation.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>17264103</pmid><doi>10.1093/humrep/del503</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | 3',5'-Cyclic-AMP Phosphodiesterases - physiology Adult Biological and medical sciences Cyclic AMP - biosynthesis Cyclic Nucleotide Phosphodiesterases, Type 4 Cyclooxygenase 1 - biosynthesis Cyclooxygenase 2 - biosynthesis Female Follicular Phase - physiology Gynecology. Andrology. Obstetrics Humans Luteal Phase - physiology Medical sciences Menorrhagia - enzymology Menorrhagia - physiopathology Middle Aged Receptors, Prostaglandin E - physiology Reverse Transcriptase Polymerase Chain Reaction Signal Transduction - physiology |
| title | Cyclooxygenase enzyme expression and E series prostaglandin receptor signalling are enhanced in heavy menstruation |
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