A Quantitative, High-Throughput Screen for Protein Stability

In proteomic research, it is often necessary to screen a large number of polypeptides for the presence of stable structure. Described here is a technique (referred to as SUPREX, stability of unpurified proteins from rates of H/D exchange) for measuring the stability of proteins in a rapid, high-thro...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2000-07, Vol.97 (15), p.8296-8301
Hauptverfasser:	Ghaemmaghami, S., Fitzgerald, M. C., Oas, T. G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amides Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriophage lambda - chemistry Biochemistry Biological Sciences Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism Deuterons DNA-Binding Proteins Exchange rates Hydrogen Ligands Maltose - genetics Maltose - metabolism Maltose-Binding Proteins Mass spectra Peptides Plasma stability Protein stability Proteins Protons Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Repressor Proteins - chemistry Repressor Proteins - genetics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Viral Proteins - chemistry Viral Proteins - genetics Viral Regulatory and Accessory Proteins
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