Methods to determine DNA structural alterations and genetic instability
Chromosomal DNA is a dynamic structure that can adopt a variety of non-canonical (i.e., non-B) conformations. In this regard, at least 10 different forms of non-B DNA conformations have been identified; many of them have been found to be mutagenic, and associated with human disease development. Desp...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2009-05, Vol.48 (1), p.54-62 |
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| creator | Wang, Guliang Zhao, Junhua Vasquez, Karen M. |
| description | Chromosomal DNA is a dynamic structure that can adopt a variety of non-canonical (i.e., non-B) conformations. In this regard, at least 10 different forms of non-B DNA conformations have been identified; many of them have been found to be mutagenic, and associated with human disease development. Despite the importance of non-B DNA structures in genetic instability and DNA metabolic processes, mechanisms by which instability occurs remain largely undefined. The purpose of this review is to summarize current methodologies that are used to address questions in the field of non-B DNA structure-induced genetic instability. Advantages and disadvantages of each method will be discussed. A focused effort to further elucidate the mechanisms of non-B DNA-induced genetic instability will lead to a better understanding of how these structure-forming sequences contribute to the development of human disease. |
| doi_str_mv | 10.1016/j.ymeth.2009.02.012 |
| format | Article |
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In this regard, at least 10 different forms of non-B DNA conformations have been identified; many of them have been found to be mutagenic, and associated with human disease development. Despite the importance of non-B DNA structures in genetic instability and DNA metabolic processes, mechanisms by which instability occurs remain largely undefined. The purpose of this review is to summarize current methodologies that are used to address questions in the field of non-B DNA structure-induced genetic instability. Advantages and disadvantages of each method will be discussed. 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In this regard, at least 10 different forms of non-B DNA conformations have been identified; many of them have been found to be mutagenic, and associated with human disease development. Despite the importance of non-B DNA structures in genetic instability and DNA metabolic processes, mechanisms by which instability occurs remain largely undefined. The purpose of this review is to summarize current methodologies that are used to address questions in the field of non-B DNA structure-induced genetic instability. Advantages and disadvantages of each method will be discussed. A focused effort to further elucidate the mechanisms of non-B DNA-induced genetic instability will lead to a better understanding of how these structure-forming sequences contribute to the development of human disease.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Chromosome</subject><subject>Chromosome Aberrations</subject><subject>Chromosomes, Artificial, Yeast</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA Mutational Analysis</subject><subject>DNA structure</subject><subject>Genes, Reporter</subject><subject>Genetic instability</subject><subject>Genomic Instability</subject><subject>Humans</subject><subject>Mammal</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Plasmid</subject><subject>Plasmids</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Reporter gene</subject><subject>Yeast</subject><issn>1046-2023</issn><issn>1095-9130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1TAQtBAVLYVfgIRy45SwtuOkPoBUtVCQ-nGBs2U769ZPiV1sp9L79_jxnvi4cNrV7szsaoaQNxQ6CnR4v-m2C5aHjgHIDlgHlD0jJxSkaCXl8HzX90PLgPFj8jLnDUCFjGcvyDGVrBdnfDwhVzdVIk65KbGZsGBafMDm8va8ySWttqxJz42e60IXH0NudJiaewxYvG18yEUbP_uyfUWOnJ4zvj7UU_L986dvF1_a67urrxfn160VQpR2nCwXo3ZGg5HOuJFxZnrBDeKIjkkpR-60YAiWu0FYIwawxjgGSAEr-ZR83Os-rmapAwylfqgek1902qqovfp3E_yDuo9Pig2SM0GrwLuDQIo_VsxFLT5bnGcdMK5ZSeh7Sem4Q_I90qaYc0L3-woFtUtAbdSvBNQuAQVMVXsr6-3fD_7hHCyvgA97AFabnjwmla3HYHHyCW1RU_T_PfATsSSbdg</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Wang, Guliang</creator><creator>Zhao, Junhua</creator><creator>Vasquez, Karen M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20090501</creationdate><title>Methods to determine DNA structural alterations and genetic instability</title><author>Wang, Guliang ; Zhao, Junhua ; Vasquez, Karen M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-7dc357afba0b9fbf7232b453bee7ef299973fa52e0c3f65cb560cbbf20e10edc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Chromosome</topic><topic>Chromosome Aberrations</topic><topic>Chromosomes, Artificial, Yeast</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA Mutational Analysis</topic><topic>DNA structure</topic><topic>Genes, Reporter</topic><topic>Genetic instability</topic><topic>Genomic Instability</topic><topic>Humans</topic><topic>Mammal</topic><topic>Methods</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Plasmid</topic><topic>Plasmids</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Reporter gene</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Guliang</creatorcontrib><creatorcontrib>Zhao, Junhua</creatorcontrib><creatorcontrib>Vasquez, Karen M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Methods (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Guliang</au><au>Zhao, Junhua</au><au>Vasquez, Karen M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methods to determine DNA structural alterations and genetic instability</atitle><jtitle>Methods (San Diego, Calif.)</jtitle><addtitle>Methods</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>48</volume><issue>1</issue><spage>54</spage><epage>62</epage><pages>54-62</pages><issn>1046-2023</issn><eissn>1095-9130</eissn><abstract>Chromosomal DNA is a dynamic structure that can adopt a variety of non-canonical (i.e., non-B) conformations. 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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Bacteria Base Sequence Cell Line Chromosome Chromosome Aberrations Chromosomes, Artificial, Yeast DNA - chemistry DNA - genetics DNA Mutational Analysis DNA structure Genes, Reporter Genetic instability Genomic Instability Humans Mammal Methods Mice Mice, Transgenic Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Plasmid Plasmids Repetitive Sequences, Nucleic Acid Reporter gene Yeast |
| title | Methods to determine DNA structural alterations and genetic instability |
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