Sizing Subcellular Organelles and Nanoparticles Confined within Aqueous Droplets
This article describes two complementary techniques, single-particle tracking and correlation spectroscopy, for accurately sizing nanoparticles confined within picoliter volume aqueous droplets. Single-particle tracking works well with bright particles that can be continuously illuminated and imaged...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2008-05, Vol.80 (9), p.3450-3457 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Gadd, Jennifer C Kuyper, Christopher L Fujimoto, Bryant S Allen, Richard W Chiu, Daniel T |
| description | This article describes two complementary techniques, single-particle tracking and correlation spectroscopy, for accurately sizing nanoparticles confined within picoliter volume aqueous droplets. Single-particle tracking works well with bright particles that can be continuously illuminated and imaged, and we demonstrated this approach for sizing single fluorescent beads. Fluorescence correlation spectroscopy detects small intensity bursts from particles or molecules diffusing through the confocal probe volume, which works well with dim and rapidly diffusing particles or molecules; we demonstrated FCS for sizing synaptic vesicles confined in aqueous droplets. In combination with recent advances in droplet manipulations and analysis, we anticipate this capability to size single nanoparticles and molecules in free solution will complement existing tools for probing cellular systems, subcellular organelles, and nanoparticles. |
| doi_str_mv | 10.1021/ac8000385 |
| format | Article |
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Chem</addtitle><description>This article describes two complementary techniques, single-particle tracking and correlation spectroscopy, for accurately sizing nanoparticles confined within picoliter volume aqueous droplets. Single-particle tracking works well with bright particles that can be continuously illuminated and imaged, and we demonstrated this approach for sizing single fluorescent beads. Fluorescence correlation spectroscopy detects small intensity bursts from particles or molecules diffusing through the confocal probe volume, which works well with dim and rapidly diffusing particles or molecules; we demonstrated FCS for sizing synaptic vesicles confined in aqueous droplets. In combination with recent advances in droplet manipulations and analysis, we anticipate this capability to size single nanoparticles and molecules in free solution will complement existing tools for probing cellular systems, subcellular organelles, and nanoparticles.</description><subject>Algorithms</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Aqueous solutions</subject><subject>Chemistry</subject><subject>Diffusion</subject><subject>Exact sciences and technology</subject><subject>Fluorescence</subject><subject>Microfluidics - methods</subject><subject>Microscopy, Fluorescence</subject><subject>Molecules</subject><subject>Nanoparticles</subject><subject>Nanoparticles - analysis</subject><subject>Nanoparticles - chemistry</subject><subject>Organelles - chemistry</subject><subject>Rats</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrum analysis</subject><subject>Surface Properties</subject><subject>Synaptic Vesicles - chemistry</subject><subject>Water - chemistry</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkFFP2zAUhS00BB3sgT-Aokk87CFwbSeO84IEHWyTKkAqk3izbhynGIJd7GQb_HpStWqH8Ivlez8dn3MIOaBwTIHRE9QSALjMt8iI5gxSISX7REaLYcoKgF3yOcYHAEqBih2ySyUXPINyRG6m9tW6WTLtK23atm8xJNdhhm54mJigq5MrdH6OobN6MRl711hn6uSv7e6tS86ee-P7mHwPft6aLu6T7QbbaL6s7j3y-_LidvwznVz_-DU-m6SYUZ6nWBYVLbkoeV4jZAylzoZjKpAgS8AMDa3rpmoAKs3rmoFgrNGS0sJkec74Hjld6s776snU2rguYKvmwT5heFEerXq_cfZezfwfxUTJMiEGga8rgeCHDLFTD74PbvCsGC2kLFlRDNC3JaSDjzGYZv0BBbXoXq27H9jD_x1tyFXZA3C0AjBqbJuATtu45hhwxku5iJYuORs782-9x_CoRMGLXN3eTNVETC7vRH6uYKOLOm5CfDT4BuuTpq4</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Gadd, Jennifer C</creator><creator>Kuyper, Christopher L</creator><creator>Fujimoto, Bryant S</creator><creator>Allen, Richard W</creator><creator>Chiu, Daniel T</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20080501</creationdate><title>Sizing Subcellular Organelles and Nanoparticles Confined within Aqueous Droplets</title><author>Gadd, Jennifer C ; Kuyper, Christopher L ; Fujimoto, Bryant S ; Allen, Richard W ; Chiu, Daniel T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a4135-a97b1936935da042a8c4444eb080890a4ae1ddfbf00bc3dd20622fc8117e45523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Algorithms</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Aqueous solutions</topic><topic>Chemistry</topic><topic>Diffusion</topic><topic>Exact sciences and technology</topic><topic>Fluorescence</topic><topic>Microfluidics - methods</topic><topic>Microscopy, Fluorescence</topic><topic>Molecules</topic><topic>Nanoparticles</topic><topic>Nanoparticles - analysis</topic><topic>Nanoparticles - chemistry</topic><topic>Organelles - chemistry</topic><topic>Rats</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrum analysis</topic><topic>Surface Properties</topic><topic>Synaptic Vesicles - chemistry</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gadd, Jennifer C</creatorcontrib><creatorcontrib>Kuyper, Christopher L</creatorcontrib><creatorcontrib>Fujimoto, Bryant S</creatorcontrib><creatorcontrib>Allen, Richard W</creatorcontrib><creatorcontrib>Chiu, Daniel T</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gadd, Jennifer C</au><au>Kuyper, Christopher L</au><au>Fujimoto, Bryant S</au><au>Allen, Richard W</au><au>Chiu, Daniel T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sizing Subcellular Organelles and Nanoparticles Confined within Aqueous Droplets</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>80</volume><issue>9</issue><spage>3450</spage><epage>3457</epage><pages>3450-3457</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>This article describes two complementary techniques, single-particle tracking and correlation spectroscopy, for accurately sizing nanoparticles confined within picoliter volume aqueous droplets. Single-particle tracking works well with bright particles that can be continuously illuminated and imaged, and we demonstrated this approach for sizing single fluorescent beads. Fluorescence correlation spectroscopy detects small intensity bursts from particles or molecules diffusing through the confocal probe volume, which works well with dim and rapidly diffusing particles or molecules; we demonstrated FCS for sizing synaptic vesicles confined in aqueous droplets. 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| subjects | Algorithms Analytical chemistry Animals Aqueous solutions Chemistry Diffusion Exact sciences and technology Fluorescence Microfluidics - methods Microscopy, Fluorescence Molecules Nanoparticles Nanoparticles - analysis Nanoparticles - chemistry Organelles - chemistry Rats Spectrometric and optical methods Spectrometry, Fluorescence Spectrum analysis Surface Properties Synaptic Vesicles - chemistry Water - chemistry |
| title | Sizing Subcellular Organelles and Nanoparticles Confined within Aqueous Droplets |
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