Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate
Objective： To investigate the mechanism of carbapenem resistance and the occurrence ofplasmid-mediated quinolone resistance determinants qnr and aac（6＇）-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods： An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a fe...
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| Veröffentlicht in: | Journal of Zhejiang University. B. Science 2009-05, Vol.10 (5), p.348-354 |
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| description | Objective： To investigate the mechanism of carbapenem resistance and the occurrence ofplasmid-mediated quinolone resistance determinants qnr and aac（6＇）-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods： An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction （PCR）, and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins （OMPs） were examined by urea-sodium dodecyl sulfate- polyacrylamide gel electrophoresis （Urea-SDS-PAGE）. Results： Minimum inhibitory concentrations （MICs） of imipenem, mer- openem, and ertapenem for ZY 106 were 2, 4, and 16 pg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-1actamase, and E. coli transconjugant produced IMP-1. Plasrnid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrSl-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZYI06 lacked an OMP of approximately 38 kDa. Conclusion： It is the first IMP-l-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blalMP and qnrS genes as well. The blalMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and mero- penern susceptibility in E. cloacae. |
| doi_str_mv | 10.1631/jzus.B0820302 |
| format | Article |
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Methods： An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction （PCR）, and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins （OMPs） were examined by urea-sodium dodecyl sulfate- polyacrylamide gel electrophoresis （Urea-SDS-PAGE）. Results： Minimum inhibitory concentrations （MICs） of imipenem, mer- openem, and ertapenem for ZY 106 were 2, 4, and 16 pg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-1actamase, and E. coli transconjugant produced IMP-1. Plasrnid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrSl-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZYI06 lacked an OMP of approximately 38 kDa. Conclusion： It is the first IMP-l-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blalMP and qnrS genes as well. The blalMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and mero- penern susceptibility in E. cloacae.</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><identifier>DOI: 10.1631/jzus.B0820302</identifier><identifier>PMID: 19434761</identifier><language>eng</language><publisher>Heidelberg: SP Zhejiang University Press</publisher><subject>beta-Lactamases - genetics ; beta-Lactamases - metabolism ; beta-Lactams - administration & dosage ; Biomedical and Life Sciences ; Biomedicine ; Enterobacter cloacae - drug effects ; Enterobacter cloacae - enzymology ; Enterobacter cloacae - genetics ; Plasmids - genetics ; Quinolones - administration & dosage ; 内酰胺酶 ; 喹诺酮类药物 ; 耐药性 ; 质粒介导 ; 进出口 ; 阴沟肠杆菌</subject><ispartof>Journal of Zhejiang University. B. Science, 2009-05, Vol.10 (5), p.348-354</ispartof><rights>Zhejiang University and Springer-Verlag GmbH 2009</rights><rights>Copyright © 2009, Journal of Zhejiang University Science 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-b21f999b202ca3f9e1b3a3254d44d5dd1b4d96a71e9949b1614a708467b2a5d03</citedby><cites>FETCH-LOGICAL-c451t-b21f999b202ca3f9e1b3a3254d44d5dd1b4d96a71e9949b1614a708467b2a5d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86281A/86281A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676414/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676414/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51297,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19434761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Li-rong</creatorcontrib><creatorcontrib>Zhou, Hong-wei</creatorcontrib><creatorcontrib>Cai, Jia-chang</creatorcontrib><creatorcontrib>Zhang, Rong</creatorcontrib><creatorcontrib>Chen, Gong-xiang</creatorcontrib><title>Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate</title><title>Journal of Zhejiang University. B. Science</title><addtitle>J. Zhejiang Univ. Sci. B</addtitle><addtitle>Journal of Zhejiang University Science</addtitle><description>Objective： To investigate the mechanism of carbapenem resistance and the occurrence ofplasmid-mediated quinolone resistance determinants qnr and aac（6＇）-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods： An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction （PCR）, and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins （OMPs） were examined by urea-sodium dodecyl sulfate- polyacrylamide gel electrophoresis （Urea-SDS-PAGE）. Results： Minimum inhibitory concentrations （MICs） of imipenem, mer- openem, and ertapenem for ZY 106 were 2, 4, and 16 pg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-1actamase, and E. coli transconjugant produced IMP-1. Plasrnid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrSl-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZYI06 lacked an OMP of approximately 38 kDa. Conclusion： It is the first IMP-l-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blalMP and qnrS genes as well. The blalMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and mero- penern susceptibility in E. cloacae.</description><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>beta-Lactams - administration & dosage</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Enterobacter cloacae - drug effects</subject><subject>Enterobacter cloacae - enzymology</subject><subject>Enterobacter cloacae - genetics</subject><subject>Plasmids - genetics</subject><subject>Quinolones - administration & dosage</subject><subject>内酰胺酶</subject><subject>喹诺酮类药物</subject><subject>耐药性</subject><subject>质粒介导</subject><subject>进出口</subject><subject>阴沟肠杆菌</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAQxyMEoqVw5IosDty8-CtOckGipUClIjjA2Zokk60Xx961HST6Hn0RHoRnwtVu-ThwsqX5-Tfj-VfVU85WXEv-cnO9pNUpawWTTNyrjnmrBeVNK--Xu24k5XXLj6pHKW0YU4o1-mF1xDslVaP5cXXzBjMO2QZPwkS2DtJsRzrjaCHjSC4-fKKczJjBuUB__qAOhgwzJCTgR7JbrA8ueCQRk00Z_IBkLMY4Ww8-J2J9AQnGDFv0ONM7LpNzX7DQFx9GMrgAAyCxKbjS-HH1YAKX8MnhPKm-vD3_fPaeXn58d3H2-pIOquaZ9oJPXdf1gokB5NQh7yVIUatRqbEeR96rsdPQcOw61fVccwUNa5VuegH1yORJ9Wrv3S59-fOAPkdwZhvtDPG7CWDNvxVvr8w6fDNCN1pxVQQvDoIYdgumbGabBnQOPIYlGd0IqXkrCkj34BBDShGn3004M7dBmtsgzV2QhX_292R_6ENyBVjtgVRKfo3RbMISfdnWf43PDxNcBb_elTemLP_rZB0ayaQSspbyF-3cucI</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Chen, Li-rong</creator><creator>Zhou, Hong-wei</creator><creator>Cai, Jia-chang</creator><creator>Zhang, Rong</creator><creator>Chen, Gong-xiang</creator><general>SP Zhejiang University Press</general><general>Zhejiang University Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090501</creationdate><title>Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate</title><author>Chen, Li-rong ; Zhou, Hong-wei ; Cai, Jia-chang ; Zhang, Rong ; Chen, Gong-xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-b21f999b202ca3f9e1b3a3254d44d5dd1b4d96a71e9949b1614a708467b2a5d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>beta-Lactams - administration & dosage</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Enterobacter cloacae - drug effects</topic><topic>Enterobacter cloacae - enzymology</topic><topic>Enterobacter cloacae - genetics</topic><topic>Plasmids - genetics</topic><topic>Quinolones - administration & dosage</topic><topic>内酰胺酶</topic><topic>喹诺酮类药物</topic><topic>耐药性</topic><topic>质粒介导</topic><topic>进出口</topic><topic>阴沟肠杆菌</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Li-rong</creatorcontrib><creatorcontrib>Zhou, Hong-wei</creatorcontrib><creatorcontrib>Cai, Jia-chang</creatorcontrib><creatorcontrib>Zhang, Rong</creatorcontrib><creatorcontrib>Chen, Gong-xiang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Zhejiang University. B. Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Li-rong</au><au>Zhou, Hong-wei</au><au>Cai, Jia-chang</au><au>Zhang, Rong</au><au>Chen, Gong-xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate</atitle><jtitle>Journal of Zhejiang University. B. Science</jtitle><stitle>J. Zhejiang Univ. Sci. B</stitle><addtitle>Journal of Zhejiang University Science</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>10</volume><issue>5</issue><spage>348</spage><epage>354</epage><pages>348-354</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><abstract>Objective： To investigate the mechanism of carbapenem resistance and the occurrence ofplasmid-mediated quinolone resistance determinants qnr and aac（6＇）-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods： An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction （PCR）, and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins （OMPs） were examined by urea-sodium dodecyl sulfate- polyacrylamide gel electrophoresis （Urea-SDS-PAGE）. Results： Minimum inhibitory concentrations （MICs） of imipenem, mer- openem, and ertapenem for ZY 106 were 2, 4, and 16 pg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-1actamase, and E. coli transconjugant produced IMP-1. Plasrnid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrSl-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZYI06 lacked an OMP of approximately 38 kDa. Conclusion： It is the first IMP-l-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blalMP and qnrS genes as well. The blalMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and mero- penern susceptibility in E. cloacae.</abstract><cop>Heidelberg</cop><pub>SP Zhejiang University Press</pub><pmid>19434761</pmid><doi>10.1631/jzus.B0820302</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | beta-Lactamases - genetics beta-Lactamases - metabolism beta-Lactams - administration & dosage Biomedical and Life Sciences Biomedicine Enterobacter cloacae - drug effects Enterobacter cloacae - enzymology Enterobacter cloacae - genetics Plasmids - genetics Quinolones - administration & dosage 内酰胺酶 喹诺酮类药物 耐药性 质粒介导 进出口 阴沟肠杆菌 |
| title | Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate |
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