proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication

Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect spermatids seem to contain only one centriole (Fuller 1993), which functionally resembles the distal centriole. Using fluorescent centriolar markers, we identified a structure near the fly distal centriol...

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Veröffentlicht in:Genetics (Austin) 2009-05, Vol.182 (1), p.133-144
Hauptverfasser: Blachon, Stephanie, Cai, Xuyu, Roberts, Kela A, Yang, Kevin, Polyanovsky, Andrey, Church, Allen, Avidor-Reiss, Tomer
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container_issue 1
container_start_page 133
container_title Genetics (Austin)
container_volume 182
creator Blachon, Stephanie
Cai, Xuyu
Roberts, Kela A
Yang, Kevin
Polyanovsky, Andrey
Church, Allen
Avidor-Reiss, Tomer
description Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect spermatids seem to contain only one centriole (Fuller 1993), which functionally resembles the distal centriole. Using fluorescent centriolar markers, we identified a structure near the fly distal centriole that is reminiscent of a proximal centriole (i.e., proximal centriole-like, or PCL). We show that the PCL exhibits several features of daughter centrioles. First, a single PCL forms near the proximal segment of the older centriole. Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are in the PCL. Third, PCL formation depends on SAK/PLK4 and SAS-6. Using a genetic screen for PCL defect, we identified a mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter centriole initiation site (Kilburn et al. 2007) in Tetrahymena. We conclude that the PCL resembles an early intermediate structure of a forming centriole, which may explain why no typical centriolar structure is observed under electron microscopy. We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation.
doi_str_mv 10.1534/genetics.109.101709
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We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>19293139</pmid><doi>10.1534/genetics.109.101709</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
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source Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Amino acids
Animals
Animals, Genetically Modified
Cell cycle
centriolar proteins
centrioles
Centrioles - genetics
Centrioles - metabolism
Cilia - physiology
Drosophila - genetics
Drosophila - metabolism
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Investigations
Male
mutation
Proteins
proximal centrioles
spermatids
Spermatids - physiology
Spermatozoa - physiology
Studies
title proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication
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