proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication
Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect spermatids seem to contain only one centriole (Fuller 1993), which functionally resembles the distal centriole. Using fluorescent centriolar markers, we identified a structure near the fly distal centriol...
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description | Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect spermatids seem to contain only one centriole (Fuller 1993), which functionally resembles the distal centriole. Using fluorescent centriolar markers, we identified a structure near the fly distal centriole that is reminiscent of a proximal centriole (i.e., proximal centriole-like, or PCL). We show that the PCL exhibits several features of daughter centrioles. First, a single PCL forms near the proximal segment of the older centriole. Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are in the PCL. Third, PCL formation depends on SAK/PLK4 and SAS-6. Using a genetic screen for PCL defect, we identified a mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter centriole initiation site (Kilburn et al. 2007) in Tetrahymena. We conclude that the PCL resembles an early intermediate structure of a forming centriole, which may explain why no typical centriolar structure is observed under electron microscopy. We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation. |
doi_str_mv | 10.1534/genetics.109.101709 |
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Using fluorescent centriolar markers, we identified a structure near the fly distal centriole that is reminiscent of a proximal centriole (i.e., proximal centriole-like, or PCL). We show that the PCL exhibits several features of daughter centrioles. First, a single PCL forms near the proximal segment of the older centriole. Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are in the PCL. Third, PCL formation depends on SAK/PLK4 and SAS-6. Using a genetic screen for PCL defect, we identified a mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter centriole initiation site (Kilburn et al. 2007) in Tetrahymena. We conclude that the PCL resembles an early intermediate structure of a forming centriole, which may explain why no typical centriolar structure is observed under electron microscopy. We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1534/genetics.109.101709</identifier><identifier>PMID: 19293139</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Amino acids ; Animals ; Animals, Genetically Modified ; Cell cycle ; centriolar proteins ; centrioles ; Centrioles - genetics ; Centrioles - metabolism ; Cilia - physiology ; Drosophila - genetics ; Drosophila - metabolism ; Drosophila melanogaster ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Investigations ; Male ; mutation ; Proteins ; proximal centrioles ; spermatids ; Spermatids - physiology ; Spermatozoa - physiology ; Studies</subject><ispartof>Genetics (Austin), 2009-05, Vol.182 (1), p.133-144</ispartof><rights>Copyright Genetics Society of America May 2009</rights><rights>Copyright © 2009 by the Genetics Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-bd45e226c37f66328c68740480c4e8838bf492cd3487d61a6564232722e07bde3</citedby><cites>FETCH-LOGICAL-c551t-bd45e226c37f66328c68740480c4e8838bf492cd3487d61a6564232722e07bde3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19293139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blachon, Stephanie</creatorcontrib><creatorcontrib>Cai, Xuyu</creatorcontrib><creatorcontrib>Roberts, Kela A</creatorcontrib><creatorcontrib>Yang, Kevin</creatorcontrib><creatorcontrib>Polyanovsky, Andrey</creatorcontrib><creatorcontrib>Church, Allen</creatorcontrib><creatorcontrib>Avidor-Reiss, Tomer</creatorcontrib><title>proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect spermatids seem to contain only one centriole (Fuller 1993), which functionally resembles the distal centriole. Using fluorescent centriolar markers, we identified a structure near the fly distal centriole that is reminiscent of a proximal centriole (i.e., proximal centriole-like, or PCL). We show that the PCL exhibits several features of daughter centrioles. First, a single PCL forms near the proximal segment of the older centriole. Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are in the PCL. Third, PCL formation depends on SAK/PLK4 and SAS-6. Using a genetic screen for PCL defect, we identified a mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter centriole initiation site (Kilburn et al. 2007) in Tetrahymena. We conclude that the PCL resembles an early intermediate structure of a forming centriole, which may explain why no typical centriolar structure is observed under electron microscopy. We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Cell cycle</subject><subject>centriolar proteins</subject><subject>centrioles</subject><subject>Centrioles - genetics</subject><subject>Centrioles - metabolism</subject><subject>Cilia - physiology</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>Drosophila melanogaster</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Investigations</subject><subject>Male</subject><subject>mutation</subject><subject>Proteins</subject><subject>proximal centrioles</subject><subject>spermatids</subject><subject>Spermatids - physiology</subject><subject>Spermatozoa - physiology</subject><subject>Studies</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdUk2PFCEQJUbjjqO_wESJB2-9UkADfTEx62eyiQfdM2HomhlWpmmhe8c9-N9FZ3TUA6mk6tXLe_Ug5DGwc2iFfLHBAafgyzmwrj7QrLtDFtBJ0XAl4C5ZMAaqUVrAGXlQyjVjTHWtuU_OoOOdANEtyPcxp29h5yL1OEw5pIhNDF-QlinPfpoz0lDomLHUMQ0DfZ1TSeM2REfLiHnnptAX6oaeejfQgvkGqasNuks9RjqlyjT3tyd62s9jDL7upeEhubd2seCjY12Sq7dvPl-8by4_vvtw8eqy8W0LU7PqZYucKy_0WinBjVdGSyYN8xKNEWa1lh33vZBG9wqcapXkgmvOkelVj2JJXh54x3m1w_6XFhftmKvzfGuTC_bfyRC2dpNuLFdaGuCV4PmRIKevM5bJ7kLxGKMbMM3FKg0GdD3qkjz7D3id5jxUc5aDBAGyZrMk4gDy9Zgl4_qPEmD2Z7b2d7a10dlDtnXryd8mTjvHME8it2Gz3YeMttRgY4WD3e_3YLgFC0JU4NMDcO2SdZscir36xBmI-l80CK3FD6xwuvA</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Blachon, Stephanie</creator><creator>Cai, Xuyu</creator><creator>Roberts, Kela A</creator><creator>Yang, Kevin</creator><creator>Polyanovsky, Andrey</creator><creator>Church, Allen</creator><creator>Avidor-Reiss, Tomer</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090501</creationdate><title>proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication</title><author>Blachon, Stephanie ; Cai, Xuyu ; Roberts, Kela A ; Yang, Kevin ; Polyanovsky, Andrey ; Church, Allen ; Avidor-Reiss, Tomer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-bd45e226c37f66328c68740480c4e8838bf492cd3487d61a6564232722e07bde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Cell cycle</topic><topic>centriolar proteins</topic><topic>centrioles</topic><topic>Centrioles - genetics</topic><topic>Centrioles - metabolism</topic><topic>Cilia - physiology</topic><topic>Drosophila - genetics</topic><topic>Drosophila - metabolism</topic><topic>Drosophila melanogaster</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Investigations</topic><topic>Male</topic><topic>mutation</topic><topic>Proteins</topic><topic>proximal centrioles</topic><topic>spermatids</topic><topic>Spermatids - physiology</topic><topic>Spermatozoa - physiology</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blachon, Stephanie</creatorcontrib><creatorcontrib>Cai, Xuyu</creatorcontrib><creatorcontrib>Roberts, Kela A</creatorcontrib><creatorcontrib>Yang, Kevin</creatorcontrib><creatorcontrib>Polyanovsky, Andrey</creatorcontrib><creatorcontrib>Church, Allen</creatorcontrib><creatorcontrib>Avidor-Reiss, Tomer</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blachon, Stephanie</au><au>Cai, Xuyu</au><au>Roberts, Kela A</au><au>Yang, Kevin</au><au>Polyanovsky, Andrey</au><au>Church, Allen</au><au>Avidor-Reiss, Tomer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>182</volume><issue>1</issue><spage>133</spage><epage>144</epage><pages>133-144</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect spermatids seem to contain only one centriole (Fuller 1993), which functionally resembles the distal centriole. Using fluorescent centriolar markers, we identified a structure near the fly distal centriole that is reminiscent of a proximal centriole (i.e., proximal centriole-like, or PCL). We show that the PCL exhibits several features of daughter centrioles. First, a single PCL forms near the proximal segment of the older centriole. Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are in the PCL. Third, PCL formation depends on SAK/PLK4 and SAS-6. Using a genetic screen for PCL defect, we identified a mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter centriole initiation site (Kilburn et al. 2007) in Tetrahymena. We conclude that the PCL resembles an early intermediate structure of a forming centriole, which may explain why no typical centriolar structure is observed under electron microscopy. We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>19293139</pmid><doi>10.1534/genetics.109.101709</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | Amino acids Animals Animals, Genetically Modified Cell cycle centriolar proteins centrioles Centrioles - genetics Centrioles - metabolism Cilia - physiology Drosophila - genetics Drosophila - metabolism Drosophila melanogaster Drosophila Proteins - genetics Drosophila Proteins - metabolism Investigations Male mutation Proteins proximal centrioles spermatids Spermatids - physiology Spermatozoa - physiology Studies |
title | proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication |
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