Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was c...

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Veröffentlicht in:	BioMed research international 2009-01, Vol.2009 (2009), p.1-9
Hauptverfasser:	Sharma, Arishya, Ng, Tzi Bun, Wong, Jack Ho, Lin, Peng
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Beans Carbohydrates Cell Proliferation - drug effects Cells, Cultured Chemical properties Chromatography Chromatography, Affinity Chromatography, Ion Exchange Cultivars Dilution Drug Stability Female Flowers & plants Hemagglutination HIV Reverse Transcriptase - antagonists & inhibitors Humans Identification and classification Lectins Legumes Macrophages, Peritoneal - metabolism Mice Mimosaceae Mitogens - chemistry Mitogens - isolation & purification Mitogens - pharmacology Nitric Oxide - metabolism Phaseolus - chemistry Phytohemagglutinins - chemistry Phytohemagglutinins - isolation & purification Phytohemagglutinins - pharmacology Properties Proteins Studies Temperature Tryptophan - metabolism Tumor Cells, Cultured
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