TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria
Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a...
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| Veröffentlicht in: | The Journal of cell biology 2009-01, Vol.184 (2), p.215-223 |
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| container_title | The Journal of cell biology |
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| creator | N'Diaye, Elsa-Noah Branda, Catherine S Branda, Steven S Nevarez, Lisette Colonna, Marco Lowell, Clifford Hamerman, Jessica A Seaman, William E |
| description | Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2-DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow-derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2-DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2-dependent phagocytosis is impaired in Syk-deficient macrophages. This study highlights a novel role for TREM-2-DAP12 in the immune response to bacterial pathogens. |
| doi_str_mv | 10.1083/jcb.200808080 |
| format | Article |
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(SNL-NM), Albuquerque, NM (United States)</creatorcontrib><description>Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2-DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow-derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2-DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2-dependent phagocytosis is impaired in Syk-deficient macrophages. This study highlights a novel role for TREM-2-DAP12 in the immune response to bacterial pathogens.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.200808080</identifier><identifier>PMID: 19171755</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Adaptor Proteins, Signal Transducing - metabolism ; Animals ; Antibodies ; Bacteria ; Bacteria - immunology ; Bacteria - metabolism ; Bacterial Physiological Phenomena ; BASIC BIOLOGICAL SCIENCES ; Biochemistry ; Cell Biology ; Cells ; Cells, Cultured ; CHO Cells ; Cricetinae ; Cricetulus ; Cytometry ; Cytoskeleton ; Escherichia coli - metabolism ; Fluorescence ; Immunology ; Macrophages ; Membrane Glycoproteins - metabolism ; Mice ; Mice, Knockout ; Myeloid cells ; Myeloid Cells - immunology ; Myeloid Cells - metabolism ; Phagocytes ; Phagocytosis ; Phagocytosis - immunology ; Receptors ; Receptors, Immunologic - metabolism ; Rodents ; Staphylococcus aureus - metabolism</subject><ispartof>The Journal of cell biology, 2009-01, Vol.184 (2), p.215-223</ispartof><rights>Copyright Rockefeller University Press Jan 28, 2009</rights><rights>2009 N'Diaye et al. 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-771ef8247f3b0d9921b6db1c841834ffda948d24a5aff6a9479d7710a65d284b3</citedby><cites>FETCH-LOGICAL-c551t-771ef8247f3b0d9921b6db1c841834ffda948d24a5aff6a9479d7710a65d284b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19171755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1625153$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>N'Diaye, Elsa-Noah</creatorcontrib><creatorcontrib>Branda, Catherine S</creatorcontrib><creatorcontrib>Branda, Steven S</creatorcontrib><creatorcontrib>Nevarez, Lisette</creatorcontrib><creatorcontrib>Colonna, Marco</creatorcontrib><creatorcontrib>Lowell, Clifford</creatorcontrib><creatorcontrib>Hamerman, Jessica A</creatorcontrib><creatorcontrib>Seaman, William E</creatorcontrib><creatorcontrib>Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)</creatorcontrib><title>TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2-DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow-derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2-DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2-dependent phagocytosis is impaired in Syk-deficient macrophages. This study highlights a novel role for TREM-2-DAP12 in the immune response to bacterial pathogens.</description><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Bacteria</subject><subject>Bacteria - immunology</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Physiological Phenomena</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biochemistry</subject><subject>Cell Biology</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Cytometry</subject><subject>Cytoskeleton</subject><subject>Escherichia coli - metabolism</subject><subject>Fluorescence</subject><subject>Immunology</subject><subject>Macrophages</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Myeloid cells</subject><subject>Myeloid Cells - immunology</subject><subject>Myeloid Cells - metabolism</subject><subject>Phagocytes</subject><subject>Phagocytosis</subject><subject>Phagocytosis - immunology</subject><subject>Receptors</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Rodents</subject><subject>Staphylococcus aureus - metabolism</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd2L1DAUxYMo7rj66KMafBB96JqbjzZ9EWRZP2BF0N3nkKZJJ8NM000y4vz3ZuzQVUII4f5y7s05CD0HcgFEsvcb011QQuTf9QCtQHBSSeDkIVoRQqFqBRVn6ElKG0IIbzh7jM6ghQYaIVZI3fy4-lZR_DZHPww2-nHA0Ro75RCx_T1Fm5LtcRjx7mC3wffY2O02YfoO-4Q1ntZ6COaQvbl_5srutMlFTT9Fj5zeJvvsdJ6j209XN5dfquvvn79efryujBCQq6YB6yTljWMd6duWQlf3HRjJQTLuXK9bLnvKtdDO1eXStH15Q3Qteip5x87Rh1l32nc72xs75qi3aop-p-NBBe3V_5XRr9UQfilaC86IKAKvZ4GQslfJ-GzN2oRxtCYrqKkAwQr05tQlhru9TVntfDoaokcb9knVtRR1Wzxe1BZwE_ZxLA4oWqwHNresZsjEkFK0bhkXiDqGq0q4agm38C___eM9fUqzAC9mYJNKDkudll4NsONUr-a600HpIfqkbn9SAoyAkLVsW_YH8BSxzw</recordid><startdate>20090126</startdate><enddate>20090126</enddate><creator>N'Diaye, Elsa-Noah</creator><creator>Branda, Catherine S</creator><creator>Branda, Steven S</creator><creator>Nevarez, Lisette</creator><creator>Colonna, Marco</creator><creator>Lowell, Clifford</creator><creator>Hamerman, Jessica A</creator><creator>Seaman, William E</creator><general>The Rockefeller University Press</general><general>Rockefeller University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OIOZB</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>20090126</creationdate><title>TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria</title><author>N'Diaye, Elsa-Noah ; 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(SNL-NM), Albuquerque, NM (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>2009-01-26</date><risdate>2009</risdate><volume>184</volume><issue>2</issue><spage>215</spage><epage>223</epage><pages>215-223</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><abstract>Phagocytosis, which is essential for the immune response to pathogens, is initiated by specific interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifies triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2-DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow-derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2-DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2-dependent phagocytosis is impaired in Syk-deficient macrophages. This study highlights a novel role for TREM-2-DAP12 in the immune response to bacterial pathogens.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>19171755</pmid><doi>10.1083/jcb.200808080</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - metabolism Animals Antibodies Bacteria Bacteria - immunology Bacteria - metabolism Bacterial Physiological Phenomena BASIC BIOLOGICAL SCIENCES Biochemistry Cell Biology Cells Cells, Cultured CHO Cells Cricetinae Cricetulus Cytometry Cytoskeleton Escherichia coli - metabolism Fluorescence Immunology Macrophages Membrane Glycoproteins - metabolism Mice Mice, Knockout Myeloid cells Myeloid Cells - immunology Myeloid Cells - metabolism Phagocytes Phagocytosis Phagocytosis - immunology Receptors Receptors, Immunologic - metabolism Rodents Staphylococcus aureus - metabolism |
| title | TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria |
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