Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates
AIM： To investigate the prevalence of vacuolating cytotoxin （vacA）, cytotoxin associated gene A （cagA） and blood adhesion binding antigen （babA2） genotypes of Helicobacter pylori （H pylori） isolates from Cuban dyspeptic patients. METHODS： DNA was extracted from Hpylori-positive cultures taken from 1...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2009-01, Vol.15 (2), p.204-210 |
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| creator | Torres, Lino E Melián, Karelia Moreno, Arlenis Alonso, Jordis Sabatier, Carlos A Hernández, Mayrín Bermúdez, Ludisleydis Rodríguez, Boris L |
| description | AIM： To investigate the prevalence of vacuolating cytotoxin （vacA）, cytotoxin associated gene A （cagA） and blood adhesion binding antigen （babA2） genotypes of Helicobacter pylori （H pylori） isolates from Cuban dyspeptic patients. METHODS： DNA was extracted from Hpylori-positive cultures taken from 130 dyspeptic patients. Genotyping was performed by PCR, using specific primers for vacA （s1, s2, m1, m2）, cagA and babA2 genes. Endoscopic observations and histological examinations were used to determine patient pathologies. RESULTS： vacA alleles s1, s2, m1 and m2 were detected in 96 （73.8%）, 34 （26.2%）, 75 （57.7%） and 52 isolates （40%）, respectively, while the cagA gene was detected in 95 isolates （73.2%）. One hundred and seven isolates （82.3%） were babA2-positive. A significant correlation was observed between vacAs1m1 and cagA and between vacAs1ml and babA2 genotypes （P 〈 0.001 and P 〈 0.05, respectively） and between babA2 genotype and cagA status （P 〈 0.05）; but, no correlation was observed between vacAsl and babA2 genotypes. Eighty five （65.4%） and 73 （56.2%） strains were type 1 （vacAsl-cagA-positive） and ＂triplepositive＂ （vacAs1-cagA-babA2-positive）, respectively, and their presence was significantly associated with duodenal ulcer （P 〈 0.01 and P 〈 0.001, respectively）. CONCLUSION： The distribution of the main virulence factors in the Cuban strains in this study resembled that of the Western-type strains, and the more virulent H pylori isolates were significantly associated with duodenal ulcer, ulcer disease being the worst pathology observed in the group studied. |
| doi_str_mv | 10.3748/wjg.15.204 |
| format | Article |
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METHODS： DNA was extracted from Hpylori-positive cultures taken from 130 dyspeptic patients. Genotyping was performed by PCR, using specific primers for vacA （s1, s2, m1, m2）, cagA and babA2 genes. Endoscopic observations and histological examinations were used to determine patient pathologies. RESULTS： vacA alleles s1, s2, m1 and m2 were detected in 96 （73.8%）, 34 （26.2%）, 75 （57.7%） and 52 isolates （40%）, respectively, while the cagA gene was detected in 95 isolates （73.2%）. One hundred and seven isolates （82.3%） were babA2-positive. A significant correlation was observed between vacAs1m1 and cagA and between vacAs1ml and babA2 genotypes （P 〈 0.001 and P 〈 0.05, respectively） and between babA2 genotype and cagA status （P 〈 0.05）; but, no correlation was observed between vacAsl and babA2 genotypes. Eighty five （65.4%） and 73 （56.2%） strains were type 1 （vacAsl-cagA-positive） and ＂triplepositive＂ （vacAs1-cagA-babA2-positive）, respectively, and their presence was significantly associated with duodenal ulcer （P 〈 0.01 and P 〈 0.001, respectively）. CONCLUSION： The distribution of the main virulence factors in the Cuban strains in this study resembled that of the Western-type strains, and the more virulent H pylori isolates were significantly associated with duodenal ulcer, ulcer disease being the worst pathology observed in the group studied.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.15.204</identifier><identifier>PMID: 19132771</identifier><language>eng</language><publisher>United States: Department of Microbiology and Immunology,Biotechnology Division,National Centre for Scientific Research,Ciudad de La Habana,Cuba%Department of Gastroenterology,Medical and Chirurgic Research Centre (CIMEQ),Ciudad de La Habana,Cuba%Anatomopathology Department,Medical and Chirurgic Research Centre (CIMEQ),Ciudad de La Habana,Cuba</publisher><subject>Adhesins, Bacterial - genetics ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antigens, Bacterial - genetics ; babA ; Bacterial Proteins - genetics ; Base Sequence ; Brief ; cagA ; Cuba ; DNA, Bacterial - genetics ; Dyspepsia - microbiology ; Female ; Genes, Bacterial ; Genotype ; Helicobacter Infections - microbiology ; Helicobacter pylori - genetics ; Helicobacter pylori - isolation & purification ; Helicobacter pylori - pathogenicity ; Humans ; Male ; Middle Aged ; vacA ; Young Adult ; 消化系统疾病</subject><ispartof>World journal of gastroenterology : WJG, 2009-01, Vol.15 (2), p.204-210</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2009 The WJG Press and Baishideng. All rights reserved. 2009</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-967486bfba976ecabe1b7e18457c5d5bdefd6856c59d32ad00ad427e0eb99c1d3</citedby><cites>FETCH-LOGICAL-c432t-967486bfba976ecabe1b7e18457c5d5bdefd6856c59d32ad00ad427e0eb99c1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2653313/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2653313/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19132771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torres, Lino E</creatorcontrib><creatorcontrib>Melián, Karelia</creatorcontrib><creatorcontrib>Moreno, Arlenis</creatorcontrib><creatorcontrib>Alonso, Jordis</creatorcontrib><creatorcontrib>Sabatier, Carlos A</creatorcontrib><creatorcontrib>Hernández, Mayrín</creatorcontrib><creatorcontrib>Bermúdez, Ludisleydis</creatorcontrib><creatorcontrib>Rodríguez, Boris L</creatorcontrib><title>Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM： To investigate the prevalence of vacuolating cytotoxin （vacA）, cytotoxin associated gene A （cagA） and blood adhesion binding antigen （babA2） genotypes of Helicobacter pylori （H pylori） isolates from Cuban dyspeptic patients. METHODS： DNA was extracted from Hpylori-positive cultures taken from 130 dyspeptic patients. Genotyping was performed by PCR, using specific primers for vacA （s1, s2, m1, m2）, cagA and babA2 genes. Endoscopic observations and histological examinations were used to determine patient pathologies. RESULTS： vacA alleles s1, s2, m1 and m2 were detected in 96 （73.8%）, 34 （26.2%）, 75 （57.7%） and 52 isolates （40%）, respectively, while the cagA gene was detected in 95 isolates （73.2%）. One hundred and seven isolates （82.3%） were babA2-positive. A significant correlation was observed between vacAs1m1 and cagA and between vacAs1ml and babA2 genotypes （P 〈 0.001 and P 〈 0.05, respectively） and between babA2 genotype and cagA status （P 〈 0.05）; but, no correlation was observed between vacAsl and babA2 genotypes. Eighty five （65.4%） and 73 （56.2%） strains were type 1 （vacAsl-cagA-positive） and ＂triplepositive＂ （vacAs1-cagA-babA2-positive）, respectively, and their presence was significantly associated with duodenal ulcer （P 〈 0.01 and P 〈 0.001, respectively）. CONCLUSION： The distribution of the main virulence factors in the Cuban strains in this study resembled that of the Western-type strains, and the more virulent H pylori isolates were significantly associated with duodenal ulcer, ulcer disease being the worst pathology observed in the group studied.</description><subject>Adhesins, Bacterial - genetics</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, Bacterial - genetics</subject><subject>babA</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Brief</subject><subject>cagA</subject><subject>Cuba</subject><subject>DNA, Bacterial - genetics</subject><subject>Dyspepsia - microbiology</subject><subject>Female</subject><subject>Genes, Bacterial</subject><subject>Genotype</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Helicobacter pylori - pathogenicity</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>vacA</subject><subject>Young Adult</subject><subject>消化系统疾病</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkV1rFDEUhoModq3e-AMkiHhR3DUfM5PJTWFZrBWKeqHX4SRzZpp1NtkmM1v6703ZxY-rQPLw5jnnJeQ1Zyupqvbj_XZY8XolWPWELITgeinaij0lC86YWmop1Bl5kfOWMSFlLZ6TM655uVV8Qb5-T3iAEYNDGnt6ALf-QB0MawqhoxbsWtABA2bqA93MFgK9xtG7aMFNmOj-YYzJU5_jCBPml-RZD2PGV6fznPy8-vRjc728-fb5y2Z9s3SVFNNSN8W7sb0FrRp0YJFbhbytauXqrrYd9l3T1o2rdScFdIxBVwmFDK3WjnfynFwec_ez3WHnMEwJRrNPfgfpwUTw5v-X4G_NEA9GNLWUXJaAd8eAewg9hMFs45xCUTZlm4IxzQTjomDvT_-keDdjnszOZ4fjCAHjnE3TtKzVqirgxRF0KeacsP_jwpl5bOkx1_DalJYK_OZf-7_oqZYCvD2l3cYw3PniV_b9q_cjGqEr1aoyxW9P8JkR</recordid><startdate>20090114</startdate><enddate>20090114</enddate><creator>Torres, Lino E</creator><creator>Melián, Karelia</creator><creator>Moreno, Arlenis</creator><creator>Alonso, Jordis</creator><creator>Sabatier, Carlos A</creator><creator>Hernández, Mayrín</creator><creator>Bermúdez, Ludisleydis</creator><creator>Rodríguez, Boris L</creator><general>Department of Microbiology and Immunology,Biotechnology Division,National Centre for Scientific Research,Ciudad de La Habana,Cuba%Department of Gastroenterology,Medical and Chirurgic Research Centre (CIMEQ),Ciudad de La Habana,Cuba%Anatomopathology Department,Medical and Chirurgic Research Centre (CIMEQ),Ciudad de La Habana,Cuba</general><general>The WJG Press and Baishideng</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20090114</creationdate><title>Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates</title><author>Torres, Lino E ; Melián, Karelia ; Moreno, Arlenis ; Alonso, Jordis ; Sabatier, Carlos A ; Hernández, Mayrín ; Bermúdez, Ludisleydis ; Rodríguez, Boris L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-967486bfba976ecabe1b7e18457c5d5bdefd6856c59d32ad00ad427e0eb99c1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adhesins, Bacterial - genetics</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, Bacterial - genetics</topic><topic>babA</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Brief</topic><topic>cagA</topic><topic>Cuba</topic><topic>DNA, Bacterial - genetics</topic><topic>Dyspepsia - microbiology</topic><topic>Female</topic><topic>Genes, Bacterial</topic><topic>Genotype</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Helicobacter pylori - pathogenicity</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>vacA</topic><topic>Young Adult</topic><topic>消化系统疾病</topic><toplevel>online_resources</toplevel><creatorcontrib>Torres, Lino E</creatorcontrib><creatorcontrib>Melián, Karelia</creatorcontrib><creatorcontrib>Moreno, Arlenis</creatorcontrib><creatorcontrib>Alonso, Jordis</creatorcontrib><creatorcontrib>Sabatier, Carlos A</creatorcontrib><creatorcontrib>Hernández, Mayrín</creatorcontrib><creatorcontrib>Bermúdez, Ludisleydis</creatorcontrib><creatorcontrib>Rodríguez, Boris L</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torres, Lino E</au><au>Melián, Karelia</au><au>Moreno, Arlenis</au><au>Alonso, Jordis</au><au>Sabatier, Carlos A</au><au>Hernández, Mayrín</au><au>Bermúdez, Ludisleydis</au><au>Rodríguez, Boris L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2009-01-14</date><risdate>2009</risdate><volume>15</volume><issue>2</issue><spage>204</spage><epage>210</epage><pages>204-210</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM： To investigate the prevalence of vacuolating cytotoxin （vacA）, cytotoxin associated gene A （cagA） and blood adhesion binding antigen （babA2） genotypes of Helicobacter pylori （H pylori） isolates from Cuban dyspeptic patients. METHODS： DNA was extracted from Hpylori-positive cultures taken from 130 dyspeptic patients. Genotyping was performed by PCR, using specific primers for vacA （s1, s2, m1, m2）, cagA and babA2 genes. Endoscopic observations and histological examinations were used to determine patient pathologies. RESULTS： vacA alleles s1, s2, m1 and m2 were detected in 96 （73.8%）, 34 （26.2%）, 75 （57.7%） and 52 isolates （40%）, respectively, while the cagA gene was detected in 95 isolates （73.2%）. One hundred and seven isolates （82.3%） were babA2-positive. A significant correlation was observed between vacAs1m1 and cagA and between vacAs1ml and babA2 genotypes （P 〈 0.001 and P 〈 0.05, respectively） and between babA2 genotype and cagA status （P 〈 0.05）; but, no correlation was observed between vacAsl and babA2 genotypes. Eighty five （65.4%） and 73 （56.2%） strains were type 1 （vacAsl-cagA-positive） and ＂triplepositive＂ （vacAs1-cagA-babA2-positive）, respectively, and their presence was significantly associated with duodenal ulcer （P 〈 0.01 and P 〈 0.001, respectively）. CONCLUSION： The distribution of the main virulence factors in the Cuban strains in this study resembled that of the Western-type strains, and the more virulent H pylori isolates were significantly associated with duodenal ulcer, ulcer disease being the worst pathology observed in the group studied.</abstract><cop>United States</cop><pub>Department of Microbiology and Immunology,Biotechnology Division,National Centre for Scientific Research,Ciudad de La Habana,Cuba%Department of Gastroenterology,Medical and Chirurgic Research Centre (CIMEQ),Ciudad de La Habana,Cuba%Anatomopathology Department,Medical and Chirurgic Research Centre (CIMEQ),Ciudad de La Habana,Cuba</pub><pmid>19132771</pmid><doi>10.3748/wjg.15.204</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesins, Bacterial - genetics Adolescent Adult Aged Aged, 80 and over Antigens, Bacterial - genetics babA Bacterial Proteins - genetics Base Sequence Brief cagA Cuba DNA, Bacterial - genetics Dyspepsia - microbiology Female Genes, Bacterial Genotype Helicobacter Infections - microbiology Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Helicobacter pylori - pathogenicity Humans Male Middle Aged vacA Young Adult 消化系统疾病 |
| title | Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates |
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