Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo
Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. We hypothesized that hematopoietic regeneration following injury is regulated by BM ECs. BALB/c mice were tre...
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| Veröffentlicht in: | Blood 2009-02, Vol.113 (9), p.2104-2107 |
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| creator | Salter, Alice B. Meadows, Sarah K. Muramoto, Garrett G. Himburg, Heather Doan, Phuong Daher, Pamela Russell, Lauren Chen, Benny Chao, Nelson J. Chute, John P. |
| description | Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. We hypothesized that hematopoietic regeneration following injury is regulated by BM ECs. BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. TBI caused pronounced disruption of the BM vasculature, BM hypocellularity, ablation of HSCs, and pancytopenia in control mice, whereas irradiated, EPC-treated mice displayed accelerated recovery of BM sinusoidal vessels, BM cellularity, peripheral blood white blood cells (WBCs), neutrophils, and platelets, and a 4.4-fold increase in BM HSCs. Systemic administration of anti–VE-cadherin antibody significantly delayed hematologic recovery in both EPC-treated mice and irradiated, non–EPC-treated mice compared with irradiated controls. These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo. |
| doi_str_mv | 10.1182/blood-2008-06-162941 |
| format | Article |
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We hypothesized that hematopoietic regeneration following injury is regulated by BM ECs. BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. TBI caused pronounced disruption of the BM vasculature, BM hypocellularity, ablation of HSCs, and pancytopenia in control mice, whereas irradiated, EPC-treated mice displayed accelerated recovery of BM sinusoidal vessels, BM cellularity, peripheral blood white blood cells (WBCs), neutrophils, and platelets, and a 4.4-fold increase in BM HSCs. Systemic administration of anti–VE-cadherin antibody significantly delayed hematologic recovery in both EPC-treated mice and irradiated, non–EPC-treated mice compared with irradiated controls. These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood-2008-06-162941</identifier><identifier>PMID: 19141867</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blood Cell Count ; Cell Differentiation - physiology ; Endothelial Cells - physiology ; Endothelial Cells - transplantation ; Fetal Blood - cytology ; Hematopoiesis - physiology ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - physiology ; Hematopoietic Stem Cells - radiation effects ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Recovery of Function ; Stem Cell Transplantation ; Stem Cells - physiology ; Transplantation ; Whole-Body Irradiation - adverse effects</subject><ispartof>Blood, 2009-02, Vol.113 (9), p.2104-2107</ispartof><rights>2009 American Society of Hematology</rights><rights>2009 by The American Society of Hematology 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-7052fc401d7e5acd9e65b731ec71040e9958bb0c3b001466c9302432d6b452e63</citedby><cites>FETCH-LOGICAL-c527t-7052fc401d7e5acd9e65b731ec71040e9958bb0c3b001466c9302432d6b452e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19141867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salter, Alice B.</creatorcontrib><creatorcontrib>Meadows, Sarah K.</creatorcontrib><creatorcontrib>Muramoto, Garrett G.</creatorcontrib><creatorcontrib>Himburg, Heather</creatorcontrib><creatorcontrib>Doan, Phuong</creatorcontrib><creatorcontrib>Daher, Pamela</creatorcontrib><creatorcontrib>Russell, Lauren</creatorcontrib><creatorcontrib>Chen, Benny</creatorcontrib><creatorcontrib>Chao, Nelson J.</creatorcontrib><creatorcontrib>Chute, John P.</creatorcontrib><title>Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo</title><title>Blood</title><addtitle>Blood</addtitle><description>Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. 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These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo.</description><subject>Animals</subject><subject>Blood Cell Count</subject><subject>Cell Differentiation - physiology</subject><subject>Endothelial Cells - physiology</subject><subject>Endothelial Cells - transplantation</subject><subject>Fetal Blood - cytology</subject><subject>Hematopoiesis - physiology</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Hematopoietic Stem Cells - radiation effects</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Recovery of Function</subject><subject>Stem Cell Transplantation</subject><subject>Stem Cells - physiology</subject><subject>Transplantation</subject><subject>Whole-Body Irradiation - adverse effects</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1OwzAQhS0EoqVwA4RygcCMEzvNBglV_EmV2MCKhZXY09YojSvbjcTtSUlFYcPKI8-8N_M-xi4RrhGn_KZunDMpB5imIFOUvMzxiI1R8P4DOByzMUDfycsCR-wshA8AzDMuTtkIS8xxKosxe79vjYsramzVJBvvltTa6HyiqWkS2y62wbq2L8xWU0hWtK6i2zhL0eokRFoPg560a0O0cRuH8aSznTtnJ4uqCXSxfyfs7eH-dfaUzl8en2d381QLXsS0AMEXOgc0BYlKm5KkqIsMSRcIOVBZimldg87qXQApdZkB74MYWeeCk8wm7Hbw3WzrNRlNbfRVozberiv_qVxl1d9Oa1dq6TrFpUDAsjfIBwPtXQieFj9aBLWDrb5hqx1sBVINsHvZ1e-9B9Ge7uEw6tN3lrwK2lKrydieWFTG2f83fAE0t5O7</recordid><startdate>20090226</startdate><enddate>20090226</enddate><creator>Salter, Alice B.</creator><creator>Meadows, Sarah K.</creator><creator>Muramoto, Garrett G.</creator><creator>Himburg, Heather</creator><creator>Doan, Phuong</creator><creator>Daher, Pamela</creator><creator>Russell, Lauren</creator><creator>Chen, Benny</creator><creator>Chao, Nelson J.</creator><creator>Chute, John P.</creator><general>Elsevier Inc</general><general>American Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20090226</creationdate><title>Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo</title><author>Salter, Alice B. ; 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We hypothesized that hematopoietic regeneration following injury is regulated by BM ECs. BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. TBI caused pronounced disruption of the BM vasculature, BM hypocellularity, ablation of HSCs, and pancytopenia in control mice, whereas irradiated, EPC-treated mice displayed accelerated recovery of BM sinusoidal vessels, BM cellularity, peripheral blood white blood cells (WBCs), neutrophils, and platelets, and a 4.4-fold increase in BM HSCs. Systemic administration of anti–VE-cadherin antibody significantly delayed hematologic recovery in both EPC-treated mice and irradiated, non–EPC-treated mice compared with irradiated controls. 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| ispartof | Blood, 2009-02, Vol.113 (9), p.2104-2107 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Blood Cell Count Cell Differentiation - physiology Endothelial Cells - physiology Endothelial Cells - transplantation Fetal Blood - cytology Hematopoiesis - physiology Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - physiology Hematopoietic Stem Cells - radiation effects Mice Mice, Inbred BALB C Mice, Inbred C57BL Recovery of Function Stem Cell Transplantation Stem Cells - physiology Transplantation Whole-Body Irradiation - adverse effects |
| title | Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo |
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