Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells
We demonstrate the use of electron microscopy as a powerful characterization tool to identify and locate antibody-conjugated composite organic-inorganic nanoparticle (COINs) surface enhanced Raman scattering (SERS) nanoparticles on cells. U937 leukemia cells labeled with antibody CD54-conjugated COI...
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| Veröffentlicht in: | Ultramicroscopy 2008-12, Vol.109 (1), p.111-121 |
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| creator | Koh, Ai Leen Shachaf, Catherine M. Elchuri, Sailaja Nolan, Garry P. Sinclair, Robert |
| description | We demonstrate the use of electron microscopy as a powerful characterization tool to identify and locate antibody-conjugated composite organic-inorganic nanoparticle (COINs) surface enhanced Raman scattering (SERS) nanoparticles on cells. U937 leukemia cells labeled with antibody CD54-conjugated COINs were characterized in their native, hydrated state using wet scanning electron microscopy (SEM) and in their dehydrated state using high-resolution SEM. In both cases, the backscattered electron (BSE) detector was used to detect and identify the silver constituents in COINs due to its high sensitivity to atomic number variations within a specimen. The imaging and analytical capabilities in the SEM were further complemented by higher resolution transmission electron microscopy (TEM) images and scanning Auger electron spectroscopy (AES) data to give reliable and high-resolution information about nanoparticles and their binding to cell surface antigens. |
| doi_str_mv | 10.1016/j.ultramic.2008.09.004 |
| format | Article |
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| ispartof | Ultramicroscopy, 2008-12, Vol.109 (1), p.111-121 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Humans Immunoconjugates - chemistry Immunoconjugates - ultrastructure Intercellular Adhesion Molecule-1 - chemistry Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Microscopy, Electron, Scanning - methods Microscopy, Electron, Transmission - methods Scanning Auger electron spectroscopy Scanning electron microscopy SERS nanoparticles on cells Silver - chemistry Spectrum Analysis, Raman - methods Transmission electron microscopy U937 Cells - ultrastructure |
| title | Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells |
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