Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules
Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response to oxidative stress. Nodule natural senescence was characterized in alfalfa (Medicago sativa) plants nodulated by the flavodoxin-overexpressing rhizobia or the corre...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2009-02, Vol.149 (2), p.1166-1178 |
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| creator | Redondo, Francisco J. de la Peña, Teodoro Coba Morcillo, César N. Lucas, M. Mercedes Pueyo, José J. |
| description | Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response to oxidative stress. Nodule natural senescence was characterized in alfalfa (Medicago sativa) plants nodulated by the flavodoxin-overexpressing rhizobia or the corresponding control bacteria. The decline of nitrogenase activity and the nodule structural and ultrastructural alterations that are associated with nodule senescence were significantly delayed in flavodoxin expressing nodules. Substantial changes in nodule antioxidant metabolism, involving antioxidant enzymes and ascorbateglutathione cycle enzymes and metabolites, were detected in flavodoxin-containing nodules. Lipid peroxidation was also significantly lower in flavodoxin-expressing nodules than in control nodules. The observed amelioration of the oxidative balance suggests that the delay in nodule senescence was most likely due to a role of the protein in reactive oxygen species detoxification. Flavodoxin overexpression also led to high starch accumulation in nodules, without reduction of the nitrogenfixing activity. |
| doi_str_mv | 10.1104/pp.108.129601 |
| format | Article |
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Mercedes ; Pueyo, José J.</creator><creatorcontrib>Redondo, Francisco J. ; de la Peña, Teodoro Coba ; Morcillo, César N. ; Lucas, M. Mercedes ; Pueyo, José J.</creatorcontrib><description>Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response to oxidative stress. Nodule natural senescence was characterized in alfalfa (Medicago sativa) plants nodulated by the flavodoxin-overexpressing rhizobia or the corresponding control bacteria. The decline of nitrogenase activity and the nodule structural and ultrastructural alterations that are associated with nodule senescence were significantly delayed in flavodoxin expressing nodules. Substantial changes in nodule antioxidant metabolism, involving antioxidant enzymes and ascorbateglutathione cycle enzymes and metabolites, were detected in flavodoxin-containing nodules. Lipid peroxidation was also significantly lower in flavodoxin-expressing nodules than in control nodules. The observed amelioration of the oxidative balance suggests that the delay in nodule senescence was most likely due to a role of the protein in reactive oxygen species detoxification. Flavodoxin overexpression also led to high starch accumulation in nodules, without reduction of the nitrogenfixing activity.</description><identifier>ISSN: 0032-0889</identifier><identifier>ISSN: 1532-2548</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.108.129601</identifier><identifier>PMID: 19098093</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Age ; Agronomy. Soil science and plant productions ; Alfalfa ; Antioxidants ; Bacteria ; Bacteroides - genetics ; Bacteroids ; Biological and medical sciences ; Cellular senescence ; Cellular Senescence - genetics ; Cellular Senescence - physiology ; Economic plant physiology ; Flavodoxin - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; Gene Expression Regulation, Bacterial ; Medicago sativa - growth & development ; Medicago sativa - physiology ; Nitrogen Fixation ; Nitrogenase - metabolism ; Nodules ; Oxidative Stress ; Parasitism and symbiosis ; Plant physiology and development ; Plant Proteins - metabolism ; Plant Roots - growth & development ; Plant Roots - physiology ; Plants ; Plants Interacting with Other Organisms ; Plasmids ; Polymerase Chain Reaction ; Senescence and abscission ; Starches ; Symbiosis ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; Vegetative apparatus, growth and morphogenesis. Senescence</subject><ispartof>Plant physiology (Bethesda), 2009-02, Vol.149 (2), p.1166-1178</ispartof><rights>Copyright 2009 American Society of Plant Biologists</rights><rights>2009 INIST-CNRS</rights><rights>Copyright © 2009, American Society of Plant Biologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-1e1bf452036ab29bf93354606d1d150c5540135df9c909ff674ad414abf646703</citedby><cites>FETCH-LOGICAL-c474t-1e1bf452036ab29bf93354606d1d150c5540135df9c909ff674ad414abf646703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40537703$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40537703$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21199897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19098093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Redondo, Francisco J.</creatorcontrib><creatorcontrib>de la Peña, Teodoro Coba</creatorcontrib><creatorcontrib>Morcillo, César N.</creatorcontrib><creatorcontrib>Lucas, M. Mercedes</creatorcontrib><creatorcontrib>Pueyo, José J.</creatorcontrib><title>Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response to oxidative stress. Nodule natural senescence was characterized in alfalfa (Medicago sativa) plants nodulated by the flavodoxin-overexpressing rhizobia or the corresponding control bacteria. The decline of nitrogenase activity and the nodule structural and ultrastructural alterations that are associated with nodule senescence were significantly delayed in flavodoxin expressing nodules. Substantial changes in nodule antioxidant metabolism, involving antioxidant enzymes and ascorbateglutathione cycle enzymes and metabolites, were detected in flavodoxin-containing nodules. Lipid peroxidation was also significantly lower in flavodoxin-expressing nodules than in control nodules. The observed amelioration of the oxidative balance suggests that the delay in nodule senescence was most likely due to a role of the protein in reactive oxygen species detoxification. Flavodoxin overexpression also led to high starch accumulation in nodules, without reduction of the nitrogenfixing activity.</description><subject>Age</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Alfalfa</subject><subject>Antioxidants</subject><subject>Bacteria</subject><subject>Bacteroides - genetics</subject><subject>Bacteroids</subject><subject>Biological and medical sciences</subject><subject>Cellular senescence</subject><subject>Cellular Senescence - genetics</subject><subject>Cellular Senescence - physiology</subject><subject>Economic plant physiology</subject><subject>Flavodoxin - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Medicago sativa - growth & development</subject><subject>Medicago sativa - physiology</subject><subject>Nitrogen Fixation</subject><subject>Nitrogenase - metabolism</subject><subject>Nodules</subject><subject>Oxidative Stress</subject><subject>Parasitism and symbiosis</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots - growth & development</subject><subject>Plant Roots - physiology</subject><subject>Plants</subject><subject>Plants Interacting with Other Organisms</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Senescence and abscission</subject><subject>Starches</subject><subject>Symbiosis</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>Vegetative apparatus, growth and morphogenesis. Senescence</subject><issn>0032-0889</issn><issn>1532-2548</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1vGyEQhlHVqHHSHntsxaXHdWBh2eVSyXGbNpLTSP04r2aBtYnWsALWSv5Of2mwbLmNNNIMzDPvMAxC7ymZU0r41TjOKWnmtJSC0FdoRitWFmXFm9doRkiOSdPIc3QR4wMhhDLK36BzKolsiGQz9Pd-Z4J5HIOJ0XqHfY9vBth57R-tw9muQSUTvNUR3zo9KRPxcgNunX3OLlyymdTgEr4zCTo_2LjFKwPaujVOHn8xAzwZjX8ZZ6IyThkMLh8TBLXBC6Wm7TRA2vfe6w09ZMM_vU_4h9fTYOJbdJYvo3l39Jfoz83X38vvxer-2-1ysSoUr3kqqKFdz6uSMAFdKbteMlZxQYSmmlZEVRXP41e6lypP3_ei5qA55dD1gouasEv0-aA7Tt3W6PzWFGBox2C3EJ5aD7Z9mXF20679ri0FY43YCxQHARV8jMH0p1pK2v2y2nHMYdMelpX5j_83_Ecft5OBT0cAosr_EsApG09cSamUjawz9-HAPcTkwynPScXqPBh7BjFWqhs</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Redondo, Francisco J.</creator><creator>de la Peña, Teodoro Coba</creator><creator>Morcillo, César N.</creator><creator>Lucas, M. Mercedes</creator><creator>Pueyo, José J.</creator><general>American Society of Plant Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20090201</creationdate><title>Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules</title><author>Redondo, Francisco J. ; de la Peña, Teodoro Coba ; Morcillo, César N. ; Lucas, M. Mercedes ; Pueyo, José J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-1e1bf452036ab29bf93354606d1d150c5540135df9c909ff674ad414abf646703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Age</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Alfalfa</topic><topic>Antioxidants</topic><topic>Bacteria</topic><topic>Bacteroides - genetics</topic><topic>Bacteroids</topic><topic>Biological and medical sciences</topic><topic>Cellular senescence</topic><topic>Cellular Senescence - genetics</topic><topic>Cellular Senescence - physiology</topic><topic>Economic plant physiology</topic><topic>Flavodoxin - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Medicago sativa - growth & development</topic><topic>Medicago sativa - physiology</topic><topic>Nitrogen Fixation</topic><topic>Nitrogenase - metabolism</topic><topic>Nodules</topic><topic>Oxidative Stress</topic><topic>Parasitism and symbiosis</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Roots - growth & development</topic><topic>Plant Roots - physiology</topic><topic>Plants</topic><topic>Plants Interacting with Other Organisms</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Senescence and abscission</topic><topic>Starches</topic><topic>Symbiosis</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>Vegetative apparatus, growth and morphogenesis. Senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redondo, Francisco J.</creatorcontrib><creatorcontrib>de la Peña, Teodoro Coba</creatorcontrib><creatorcontrib>Morcillo, César N.</creatorcontrib><creatorcontrib>Lucas, M. Mercedes</creatorcontrib><creatorcontrib>Pueyo, José J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redondo, Francisco J.</au><au>de la Peña, Teodoro Coba</au><au>Morcillo, César N.</au><au>Lucas, M. Mercedes</au><au>Pueyo, José J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>149</volume><issue>2</issue><spage>1166</spage><epage>1178</epage><pages>1166-1178</pages><issn>0032-0889</issn><issn>1532-2548</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Sinorhizobium meliloti cells were engineered to overexpress Anabaena variabilis flavodoxin, a protein that is involved in the response to oxidative stress. Nodule natural senescence was characterized in alfalfa (Medicago sativa) plants nodulated by the flavodoxin-overexpressing rhizobia or the corresponding control bacteria. The decline of nitrogenase activity and the nodule structural and ultrastructural alterations that are associated with nodule senescence were significantly delayed in flavodoxin expressing nodules. Substantial changes in nodule antioxidant metabolism, involving antioxidant enzymes and ascorbateglutathione cycle enzymes and metabolites, were detected in flavodoxin-containing nodules. Lipid peroxidation was also significantly lower in flavodoxin-expressing nodules than in control nodules. The observed amelioration of the oxidative balance suggests that the delay in nodule senescence was most likely due to a role of the protein in reactive oxygen species detoxification. Flavodoxin overexpression also led to high starch accumulation in nodules, without reduction of the nitrogenfixing activity.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>19098093</pmid><doi>10.1104/pp.108.129601</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Age Agronomy. Soil science and plant productions Alfalfa Antioxidants Bacteria Bacteroides - genetics Bacteroids Biological and medical sciences Cellular senescence Cellular Senescence - genetics Cellular Senescence - physiology Economic plant physiology Flavodoxin - genetics Fundamental and applied biological sciences. Psychology Gene Amplification Gene Expression Regulation, Bacterial Medicago sativa - growth & development Medicago sativa - physiology Nitrogen Fixation Nitrogenase - metabolism Nodules Oxidative Stress Parasitism and symbiosis Plant physiology and development Plant Proteins - metabolism Plant Roots - growth & development Plant Roots - physiology Plants Plants Interacting with Other Organisms Plasmids Polymerase Chain Reaction Senescence and abscission Starches Symbiosis Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Vegetative apparatus, growth and morphogenesis. Senescence |
| title | Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules |
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