unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

Toward the expansion of the genetic alphabet, we present an unnatural base pair system for efficient PCR amplification, enabling the site-specific incorporation of extra functional components into DNA. This system can be applied to conventional PCR protocols employing DNA templates containing unnatu...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nucleic acids research 2009-02, Vol.37 (2), p.e14-e14
Hauptverfasser:	Kimoto, Michiko, Kawai, Rie, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkynes - chemistry Base Pairing DNA - analysis DNA - chemistry Imidazoles - chemistry Methods Online Nucleotides - chemistry Polymerase Chain Reaction Pyridines - chemistry Pyrroles - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!