Mutations in the Fourth EGF-like Domain Affect Thrombomodulin-Induced Changes in the Active Site of Thrombin

A number of alanine and more conservative mutants of residues in the fourth domain of thrombomodulin (TM) were prepared and assayed for protein C activation and for thrombin binding. Several of the alanine mutations appeared to cause misfolding or structural defects as assessed by poor expression an...

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