Enzyme-labeled Antigen Method: Histochemical Detection of Antigen-specific Antibody-producing Cells in Tissue Sections of Rats Immunized With Horseradish Peroxidase, Ovalbumin, or Keyhole Limpet Hemocyanin

The enzyme-labeled antigen method is a histochemical technique that visualizes antigen-specific antibody-producing cells in tissue sections, originally documented in 1968. In this study, we attempted to reemerge this hidden but potentially useful method in rat models immunized with horseradish perox...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 2009-02, Vol.57 (2), p.101-111
Hauptverfasser:	Mizutani, Yasuyoshi, Tsuge, Shinya, Shiogama, Kazuya, Shimomura, Ryoichi, Kamoshida, Shingo, Inada, Ken-ichi, Tsutsumi, Yutaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Specificity Antibody-Producing Cells - cytology Antibody-Producing Cells - immunology Antigens Endopeptidase K Fixatives Formaldehyde Frozen Sections Hemocyanins - immunology Histocytochemistry Horseradish Peroxidase - immunology Immunization Indicators and Reagents Lymph Nodes - cytology Lymph Nodes - immunology Male Organ Specificity Ovalbumin - immunology Paraffin Embedding Plasma Cells - cytology Plasma Cells - immunology Polymers Rats Rats, Sprague-Dawley Spleen - cytology Spleen - immunology
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