novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila
Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryot...
Gespeichert in:
| Veröffentlicht in: | The FASEB journal 2009-01, Vol.23 (1), p.107-113 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 113 |
|---|---|
| container_issue | 1 |
| container_start_page | 107 |
| container_title | The FASEB journal |
| container_volume | 23 |
| creator | Hirosawa-Takamori, Mitsuko Ossipov, Dmitri Novoselov, Sergey V Turanov, Anton A Zhang, Yan Gladyshev, Vadim N Krol, Alain Vorbrüggen, Gerd Jäckle, Herbert |
| description | Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.--Hirosawa-Takamori, M., Ossipov, D., Novoselov, S.V., Turanov, A.A., Zhang, Y., Gladyshev, V.N., Krol, A., Vorbrüggen, G., Jäckle, H. A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in DROSOPHILA: |
| doi_str_mv | 10.1096/fj.08-116640 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2626620</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>FSB2FJ08116640</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4580-1e9671fbe1c5950a900a0ae562e443f73ad8fa0c1007342fd92fcd7e05992d743</originalsourceid><addsrcrecordid>eNp9kc1v2yAYh9G0ac263XbeuE6a2xdsY3yZlHVLuyrSDl3OiGKIiQhY4KTKtX95SV11H4ed-Hif3yNeXoTeEzgj0LJzszkDXhDCWAUv0IzUJRSMM3iJZsBbWjBW8hP0JqUNABAg7DU6IbxpaFnVM3Tvw147nEa9xS6EAavgxxgc1k5vtR-LTg_ad3mHV5dzHLXsirGPYbfucTo8xu7s2IfdiMcofXJytMHLbMwCH9QRsV5j61WIQ4iP5XzC32JIYeitk2_RKyNd0u-e1lO0Wnz_dXFVLH9e_riYLwtV1RwKolvWEHOriarbGmQLIEHqmlFdVaVpStlxI0ERgKasqOlaalTXaKjblnZNVZ6iL5N32N1udadyT1E6MUS7lfEggrTi74q3vViHvaCMMkYhCz5Ngv6f2NV8KY53AGVFGlrtSWY_T6zKfaaozXOAgDjOTZiNAC6muWX8w59v-w0_DSoDfALurNOH_8rE4uYrXVwDf3Z_nKJGBiHX0SaxuqFASiB1U_P8eQ_oI7E4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Hirosawa-Takamori, Mitsuko ; Ossipov, Dmitri ; Novoselov, Sergey V ; Turanov, Anton A ; Zhang, Yan ; Gladyshev, Vadim N ; Krol, Alain ; Vorbrüggen, Gerd ; Jäckle, Herbert</creator><creatorcontrib>Hirosawa-Takamori, Mitsuko ; Ossipov, Dmitri ; Novoselov, Sergey V ; Turanov, Anton A ; Zhang, Yan ; Gladyshev, Vadim N ; Krol, Alain ; Vorbrüggen, Gerd ; Jäckle, Herbert</creatorcontrib><description>Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.--Hirosawa-Takamori, M., Ossipov, D., Novoselov, S.V., Turanov, A.A., Zhang, Y., Gladyshev, V.N., Krol, A., Vorbrüggen, G., Jäckle, H. A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in DROSOPHILA:</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.08-116640</identifier><identifier>PMID: 18772345</identifier><language>eng</language><publisher>United States: The Federation of American Societies for Experimental Biology</publisher><subject>Amino Acid Sequence ; Animals ; Biochemistry, Molecular Biology ; biosynthesis ; Cellular Biology ; Codon, Terminator - metabolism ; conserved ; Drosophila - metabolism ; Drosophila Proteins - chemistry ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; eEFsec ; GAP factor ; GAPsec mutant ; Gene Expression Profiling ; Gene Expression Regulation - physiology ; GTPase-Activating Proteins - chemistry ; GTPase-Activating Proteins - genetics ; GTPase-Activating Proteins - metabolism ; Inverted Repeat Sequences - physiology ; Life Sciences ; Mice ; Molecular Sequence Data ; NIH 3T3 Cells ; Organisms, Genetically Modified ; Research Communications ; Selenocysteine - metabolism ; selenoprotein ; Two-Hybrid System Techniques</subject><ispartof>The FASEB journal, 2009-01, Vol.23 (1), p.107-113</ispartof><rights>FASEB</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2009 FASEB 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4580-1e9671fbe1c5950a900a0ae562e443f73ad8fa0c1007342fd92fcd7e05992d743</citedby><cites>FETCH-LOGICAL-c4580-1e9671fbe1c5950a900a0ae562e443f73ad8fa0c1007342fd92fcd7e05992d743</cites><orcidid>0000-0003-0744-6198</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.08-116640$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.08-116640$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18772345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00341724$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Hirosawa-Takamori, Mitsuko</creatorcontrib><creatorcontrib>Ossipov, Dmitri</creatorcontrib><creatorcontrib>Novoselov, Sergey V</creatorcontrib><creatorcontrib>Turanov, Anton A</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Gladyshev, Vadim N</creatorcontrib><creatorcontrib>Krol, Alain</creatorcontrib><creatorcontrib>Vorbrüggen, Gerd</creatorcontrib><creatorcontrib>Jäckle, Herbert</creatorcontrib><title>novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.--Hirosawa-Takamori, M., Ossipov, D., Novoselov, S.V., Turanov, A.A., Zhang, Y., Gladyshev, V.N., Krol, A., Vorbrüggen, G., Jäckle, H. A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in DROSOPHILA:</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biochemistry, Molecular Biology</subject><subject>biosynthesis</subject><subject>Cellular Biology</subject><subject>Codon, Terminator - metabolism</subject><subject>conserved</subject><subject>Drosophila - metabolism</subject><subject>Drosophila Proteins - chemistry</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>eEFsec</subject><subject>GAP factor</subject><subject>GAPsec mutant</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - physiology</subject><subject>GTPase-Activating Proteins - chemistry</subject><subject>GTPase-Activating Proteins - genetics</subject><subject>GTPase-Activating Proteins - metabolism</subject><subject>Inverted Repeat Sequences - physiology</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>NIH 3T3 Cells</subject><subject>Organisms, Genetically Modified</subject><subject>Research Communications</subject><subject>Selenocysteine - metabolism</subject><subject>selenoprotein</subject><subject>Two-Hybrid System Techniques</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v2yAYh9G0ac263XbeuE6a2xdsY3yZlHVLuyrSDl3OiGKIiQhY4KTKtX95SV11H4ed-Hif3yNeXoTeEzgj0LJzszkDXhDCWAUv0IzUJRSMM3iJZsBbWjBW8hP0JqUNABAg7DU6IbxpaFnVM3Tvw147nEa9xS6EAavgxxgc1k5vtR-LTg_ad3mHV5dzHLXsirGPYbfucTo8xu7s2IfdiMcofXJytMHLbMwCH9QRsV5j61WIQ4iP5XzC32JIYeitk2_RKyNd0u-e1lO0Wnz_dXFVLH9e_riYLwtV1RwKolvWEHOriarbGmQLIEHqmlFdVaVpStlxI0ERgKasqOlaalTXaKjblnZNVZ6iL5N32N1udadyT1E6MUS7lfEggrTi74q3vViHvaCMMkYhCz5Ngv6f2NV8KY53AGVFGlrtSWY_T6zKfaaozXOAgDjOTZiNAC6muWX8w59v-w0_DSoDfALurNOH_8rE4uYrXVwDf3Z_nKJGBiHX0SaxuqFASiB1U_P8eQ_oI7E4</recordid><startdate>200901</startdate><enddate>200901</enddate><creator>Hirosawa-Takamori, Mitsuko</creator><creator>Ossipov, Dmitri</creator><creator>Novoselov, Sergey V</creator><creator>Turanov, Anton A</creator><creator>Zhang, Yan</creator><creator>Gladyshev, Vadim N</creator><creator>Krol, Alain</creator><creator>Vorbrüggen, Gerd</creator><creator>Jäckle, Herbert</creator><general>The Federation of American Societies for Experimental Biology</general><general>Federation of American Societies for Experimental Biology</general><general>Federation of American Society of Experimental Biology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0744-6198</orcidid></search><sort><creationdate>200901</creationdate><title>novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila</title><author>Hirosawa-Takamori, Mitsuko ; Ossipov, Dmitri ; Novoselov, Sergey V ; Turanov, Anton A ; Zhang, Yan ; Gladyshev, Vadim N ; Krol, Alain ; Vorbrüggen, Gerd ; Jäckle, Herbert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4580-1e9671fbe1c5950a900a0ae562e443f73ad8fa0c1007342fd92fcd7e05992d743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biochemistry, Molecular Biology</topic><topic>biosynthesis</topic><topic>Cellular Biology</topic><topic>Codon, Terminator - metabolism</topic><topic>conserved</topic><topic>Drosophila - metabolism</topic><topic>Drosophila Proteins - chemistry</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>eEFsec</topic><topic>GAP factor</topic><topic>GAPsec mutant</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - physiology</topic><topic>GTPase-Activating Proteins - chemistry</topic><topic>GTPase-Activating Proteins - genetics</topic><topic>GTPase-Activating Proteins - metabolism</topic><topic>Inverted Repeat Sequences - physiology</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>NIH 3T3 Cells</topic><topic>Organisms, Genetically Modified</topic><topic>Research Communications</topic><topic>Selenocysteine - metabolism</topic><topic>selenoprotein</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hirosawa-Takamori, Mitsuko</creatorcontrib><creatorcontrib>Ossipov, Dmitri</creatorcontrib><creatorcontrib>Novoselov, Sergey V</creatorcontrib><creatorcontrib>Turanov, Anton A</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Gladyshev, Vadim N</creatorcontrib><creatorcontrib>Krol, Alain</creatorcontrib><creatorcontrib>Vorbrüggen, Gerd</creatorcontrib><creatorcontrib>Jäckle, Herbert</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hirosawa-Takamori, Mitsuko</au><au>Ossipov, Dmitri</au><au>Novoselov, Sergey V</au><au>Turanov, Anton A</au><au>Zhang, Yan</au><au>Gladyshev, Vadim N</au><au>Krol, Alain</au><au>Vorbrüggen, Gerd</au><au>Jäckle, Herbert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2009-01</date><risdate>2009</risdate><volume>23</volume><issue>1</issue><spage>107</spage><epage>113</epage><pages>107-113</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.--Hirosawa-Takamori, M., Ossipov, D., Novoselov, S.V., Turanov, A.A., Zhang, Y., Gladyshev, V.N., Krol, A., Vorbrüggen, G., Jäckle, H. A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in DROSOPHILA:</abstract><cop>United States</cop><pub>The Federation of American Societies for Experimental Biology</pub><pmid>18772345</pmid><doi>10.1096/fj.08-116640</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0744-6198</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0892-6638 |
| ispartof | The FASEB journal, 2009-01, Vol.23 (1), p.107-113 |
| issn | 0892-6638 1530-6860 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2626620 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Biochemistry, Molecular Biology biosynthesis Cellular Biology Codon, Terminator - metabolism conserved Drosophila - metabolism Drosophila Proteins - chemistry Drosophila Proteins - genetics Drosophila Proteins - metabolism eEFsec GAP factor GAPsec mutant Gene Expression Profiling Gene Expression Regulation - physiology GTPase-Activating Proteins - chemistry GTPase-Activating Proteins - genetics GTPase-Activating Proteins - metabolism Inverted Repeat Sequences - physiology Life Sciences Mice Molecular Sequence Data NIH 3T3 Cells Organisms, Genetically Modified Research Communications Selenocysteine - metabolism selenoprotein Two-Hybrid System Techniques |
| title | novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T19%3A34%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=novel%20stem%20loop%20control%20element-dependent%20UGA%20read-through%20system%20without%20translational%20selenocysteine%20incorporation%20in%20Drosophila&rft.jtitle=The%20FASEB%20journal&rft.au=Hirosawa-Takamori,%20Mitsuko&rft.date=2009-01&rft.volume=23&rft.issue=1&rft.spage=107&rft.epage=113&rft.pages=107-113&rft.issn=0892-6638&rft.eissn=1530-6860&rft_id=info:doi/10.1096/fj.08-116640&rft_dat=%3Cwiley_pubme%3EFSB2FJ08116640%3C/wiley_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/18772345&rfr_iscdi=true |