Laser capture microdissection and real-time PCR for measuring mRNA in giant cells induced by Meloidogyne javanica

The techniques of laser capture microdissection and quantitative RT-PCR were investigated as methods for measuring mRNA in giant cells induced by Meloidogyne javanica. Laser capture microdissection allowed precise sampling of giant cells at 1 to 3 weeks after inoculation. The expression of three gen...

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Veröffentlicht in:	Journal of nematology 2005-09, Vol.37 (3), p.308-312
Hauptverfasser:	He, B, Magill, C, Starr, J.L
Format:	Artikel
Sprache:	eng
Schlagworte:	gene expression giant cells Host-Parasite Relations infection laboratory techniques lasers Meloidogyne javanica messenger RNA quantitative analysis reverse transcriptase polymerase chain reaction root-knot nematodes roots Solanum lycopersicum var. lycopersicum tomatoes
Online-Zugang:	Volltext
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