Identification of E2F1 as a positive transcriptional regulator for δ-catenin
δ-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate δ-catenin expression in cancer. Using a human δ-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect δ-catenin tr...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-05, Vol.369 (2), p.414-420 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kim, Kwonseop Oh, Minsoo Ki, Hyunkyoung Wang, Tao Bareiss, Sonja Fini, M. Elizabeth Li, Dawei Lu, Qun |
| description | δ-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate δ-catenin expression in cancer. Using a human δ-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect
δ-catenin transcription. Among β-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased δ-catenin–luciferase activities while β-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of δ-catenin–luciferase activities induced by E2F1 but did not interact with δ-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of δ-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on δ-catenin expression were observed only in human cancer cells expressing abundant endogenous δ-catenin. These studies identify E2F1 as a positive transcriptional regulator for δ-catenin, but further suggest the presence of strong negative regulator(s) for δ-catenin in prostate cancer cells with minimal endogenous δ-catenin expression. |
| doi_str_mv | 10.1016/j.bbrc.2008.02.069 |
| format | Article |
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δ-catenin transcription. Among β-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased δ-catenin–luciferase activities while β-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of δ-catenin–luciferase activities induced by E2F1 but did not interact with δ-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of δ-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on δ-catenin expression were observed only in human cancer cells expressing abundant endogenous δ-catenin. These studies identify E2F1 as a positive transcriptional regulator for δ-catenin, but further suggest the presence of strong negative regulator(s) for δ-catenin in prostate cancer cells with minimal endogenous δ-catenin expression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2008.02.069</identifier><identifier>PMID: 18302937</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cancer ; Catenin ; Catenins ; Cell Adhesion Molecules - metabolism ; Cell Line, Tumor ; E2F1 ; E2F1 Transcription Factor - genetics ; E2F1 Transcription Factor - metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; LEF-1 ; Male ; Notch ; Phosphoproteins - metabolism ; Prostate ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Signal Transduction ; Transcription ; Transcriptional Activation ; Wnt ; δ-Catenin/NPRAP</subject><ispartof>Biochemical and biophysical research communications, 2008-05, Vol.369 (2), p.414-420</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-8b82c3f79e9cc4e2a87960655e892052afa594b77272724f12654b24f6a5d7493</citedby><cites>FETCH-LOGICAL-c484t-8b82c3f79e9cc4e2a87960655e892052afa594b77272724f12654b24f6a5d7493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2008.02.069$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,778,782,883,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18302937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Kwonseop</creatorcontrib><creatorcontrib>Oh, Minsoo</creatorcontrib><creatorcontrib>Ki, Hyunkyoung</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Bareiss, Sonja</creatorcontrib><creatorcontrib>Fini, M. Elizabeth</creatorcontrib><creatorcontrib>Li, Dawei</creatorcontrib><creatorcontrib>Lu, Qun</creatorcontrib><title>Identification of E2F1 as a positive transcriptional regulator for δ-catenin</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>δ-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate δ-catenin expression in cancer. Using a human δ-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect
δ-catenin transcription. Among β-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased δ-catenin–luciferase activities while β-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of δ-catenin–luciferase activities induced by E2F1 but did not interact with δ-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of δ-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on δ-catenin expression were observed only in human cancer cells expressing abundant endogenous δ-catenin. These studies identify E2F1 as a positive transcriptional regulator for δ-catenin, but further suggest the presence of strong negative regulator(s) for δ-catenin in prostate cancer cells with minimal endogenous δ-catenin expression.</description><subject>Cancer</subject><subject>Catenin</subject><subject>Catenins</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Line, Tumor</subject><subject>E2F1</subject><subject>E2F1 Transcription Factor - genetics</subject><subject>E2F1 Transcription Factor - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>LEF-1</subject><subject>Male</subject><subject>Notch</subject><subject>Phosphoproteins - metabolism</subject><subject>Prostate</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Signal Transduction</subject><subject>Transcription</subject><subject>Transcriptional Activation</subject><subject>Wnt</subject><subject>δ-Catenin/NPRAP</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFq3DAQhkVoaLZJXyCH4lNvdkdjWbYgFEpImkBKLgnkJmR5nGjxWhvJu5D36nP0mSqzS9JeWoSQQN_8zOhj7JRDwYHLL8uibYMtEKApAAuQ6oAtOCjIkYN4xxYAIHNU_OGIfYhxCcC5kOo9O-JNCajKesF-XHc0Tq531kzOj5nvswu85JmJmcnWPrrJbSmbghmjDW49M2bIAj1uBjP5kPVp__qZp2oa3XjCDnszRPq4P4_Z_eXF3flVfnP7_fr8201uRSOmvGkbtGVfK1LWCkLT1EqCrCpqFEKFpjeVEm1d47xEz1FWok0XaaquFqo8Zl93uetNu6LOphGCGfQ6uJUJL9obp_9-Gd2TfvRbjZILASIFfN4HBP-8oTjplYuWhsGM5DdR1yBQlCX-F-SqTi6qGcQdaIOPMVD_2g0HPevSSz3r0rMuDaiTrlT06c853kr2fhJwtgMo_ebWUdDROhotdS6QnXTn3b_yfwOG46cE</recordid><startdate>20080502</startdate><enddate>20080502</enddate><creator>Kim, Kwonseop</creator><creator>Oh, Minsoo</creator><creator>Ki, Hyunkyoung</creator><creator>Wang, Tao</creator><creator>Bareiss, Sonja</creator><creator>Fini, M. Elizabeth</creator><creator>Li, Dawei</creator><creator>Lu, Qun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080502</creationdate><title>Identification of E2F1 as a positive transcriptional regulator for δ-catenin</title><author>Kim, Kwonseop ; Oh, Minsoo ; Ki, Hyunkyoung ; Wang, Tao ; Bareiss, Sonja ; Fini, M. Elizabeth ; Li, Dawei ; Lu, Qun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-8b82c3f79e9cc4e2a87960655e892052afa594b77272724f12654b24f6a5d7493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cancer</topic><topic>Catenin</topic><topic>Catenins</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Line, Tumor</topic><topic>E2F1</topic><topic>E2F1 Transcription Factor - genetics</topic><topic>E2F1 Transcription Factor - metabolism</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>LEF-1</topic><topic>Male</topic><topic>Notch</topic><topic>Phosphoproteins - metabolism</topic><topic>Prostate</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Signal Transduction</topic><topic>Transcription</topic><topic>Transcriptional Activation</topic><topic>Wnt</topic><topic>δ-Catenin/NPRAP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Kwonseop</creatorcontrib><creatorcontrib>Oh, Minsoo</creatorcontrib><creatorcontrib>Ki, Hyunkyoung</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Bareiss, Sonja</creatorcontrib><creatorcontrib>Fini, M. 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Elizabeth</au><au>Li, Dawei</au><au>Lu, Qun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of E2F1 as a positive transcriptional regulator for δ-catenin</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-05-02</date><risdate>2008</risdate><volume>369</volume><issue>2</issue><spage>414</spage><epage>420</epage><pages>414-420</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>δ-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate δ-catenin expression in cancer. Using a human δ-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect
δ-catenin transcription. Among β-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased δ-catenin–luciferase activities while β-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of δ-catenin–luciferase activities induced by E2F1 but did not interact with δ-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of δ-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on δ-catenin expression were observed only in human cancer cells expressing abundant endogenous δ-catenin. These studies identify E2F1 as a positive transcriptional regulator for δ-catenin, but further suggest the presence of strong negative regulator(s) for δ-catenin in prostate cancer cells with minimal endogenous δ-catenin expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18302937</pmid><doi>10.1016/j.bbrc.2008.02.069</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cancer Catenin Catenins Cell Adhesion Molecules - metabolism Cell Line, Tumor E2F1 E2F1 Transcription Factor - genetics E2F1 Transcription Factor - metabolism Gene Expression Regulation, Neoplastic Humans LEF-1 Male Notch Phosphoproteins - metabolism Prostate Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Signal Transduction Transcription Transcriptional Activation Wnt δ-Catenin/NPRAP |
| title | Identification of E2F1 as a positive transcriptional regulator for δ-catenin |
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