Efficient Isolation of Soluble Intracellular Single-chain Antibodies using the Twin-arginine Translocation Machinery

One of the most commonly used recombinant antibody formats is the single-chain variable fragment (scFv) that consists of the antibody variable heavy chain connected to the variable light chain by a flexible linker. Since disulfide bonds are often necessary for scFv folding, it can be challenging to...

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Veröffentlicht in:Journal of molecular biology 2009-01, Vol.385 (1), p.299-311
Hauptverfasser: Fisher, Adam C., DeLisa, Matthew P.
Format: Artikel
Sprache:eng
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