Characterization and Resolution of Evaporation-Mediated Osmolality Shifts That Constrain Microfluidic Cell Culture in Poly(dimethylsiloxane) Devices

Evaporation is a critical problem when handling submicroliter volumes of fluids. This paper characterizes this problem as it applies to microfluidic cell culture in poly(dimethylsiloxane) (PDMS) devices and provides a practical solution. Evaporation-mediated osmolality shifts through PDMS membranes...

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Veröffentlicht in:	Analytical chemistry (Washington) 2007-02, Vol.79 (3), p.1126-1134
Hauptverfasser:	Heo, Yun Seok, Cabrera, Lourdes M, Song, Jonathan W, Futai, Nobuyuki, Tung, Yi-Chung, Smith, Gary D, Takayama, Shuichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Cell culture Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Chemistry Cytological Techniques Diffusion Dimethylpolysiloxanes Embryo, Mammalian Endothelial Cells Exact sciences and technology Humans Membranes, Artificial Mice Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Osmosis
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