Development of analytical methods for multiplex bio-assay with inductively coupled plasma mass spectrometry

Development of analytical methods for multiplex bio-assay with inductively coupled plasma mass spectrometry

Advances in the development of highly multiplexed bio-analytical assays with inductively coupled plasma mass spectrometry (ICP-MS) detection are discussed. Use of novel reagents specifically designed for immunological methods utilizing elemental analysis is presented. The major steps of method devel...

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Veröffentlicht in:Journal of analytical atomic spectrometry 2008-01, Vol.23 (4), p.463-469
Hauptverfasser: Ornatsky, Olga I, Kinach, Robert, Bandura, Dmitry R, Lou, Xudong, Tanner, Scott D, Baranov, Vladimir I, Nitz, Mark, Winnik, Mitchell A
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container_end_page 469
container_issue 4
container_start_page 463
container_title Journal of analytical atomic spectrometry
container_volume 23
creator Ornatsky, Olga I
Kinach, Robert
Bandura, Dmitry R
Lou, Xudong
Tanner, Scott D
Baranov, Vladimir I
Nitz, Mark
Winnik, Mitchell A
description Advances in the development of highly multiplexed bio-analytical assays with inductively coupled plasma mass spectrometry (ICP-MS) detection are discussed. Use of novel reagents specifically designed for immunological methods utilizing elemental analysis is presented. The major steps of method development, including selection of elements for tags, validation of tagged reagents, and examples of multiplexed assays, are considered in detail. The paper further describes experimental protocols for elemental tagging of antibodies, immunostaining of live and fixed human leukemia cells, and preparation of samples for ICP-MS analysis. Quantitative analysis of surface antigens on model cell lines using a cocktail of seven lanthanide labeled antibodies demonstrated high specificity and concordance with conventional immunophenotyping.
doi_str_mv 10.1039/b710510j
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title Development of analytical methods for multiplex bio-assay with inductively coupled plasma mass spectrometry
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