Measurement of cardiac function using pressure–volume conductance catheter technique in mice and rats
Ventricular pressure–volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterizati...
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| Veröffentlicht in: | Nature protocols 2008-01, Vol.3 (9), p.1422-1434 |
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| creator | Pacher, Pál Nagayama, Takahiro Mukhopadhyay, Partha Bátkai, Sándor Kass, David A |
| description | Ventricular pressure–volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. The completion of the protocol takes 1–4 h (depending on the experimental design/end points). |
| doi_str_mv | 10.1038/nprot.2008.138 |
| format | Article |
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Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. 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Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. 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| subjects | Analytical Chemistry Anesthesia Animals Biological Techniques Biomedical and Life Sciences Blood Calibration Cardiac Catheterization - instrumentation Cardiac Catheterization - methods Cardiac function Cardiomegaly - physiopathology Cardiovascular system Catheters Computational Biology/Bioinformatics Electrodes Experimental design Genetic engineering Heart Life Sciences Medical instruments Mice Microarrays Organic Chemistry Physiology Protocol Rats Rodents Ventricular Function, Left - physiology |
| title | Measurement of cardiac function using pressure–volume conductance catheter technique in mice and rats |
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