Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster
The Pc locus of sorghum (Sorghum bicolor) determines dominant sensitivity to a host-selective toxin produced by the fungal pathogen Periconia circinata. The Pc region was cloned by a map-based approach and found to contain three tandemly repeated genes with the structures of nucleotide binding site-...
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| Veröffentlicht in: | Genome Research 2008-12, Vol.18 (12), p.1918-1923 |
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| container_end_page | 1923 |
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| container_issue | 12 |
| container_start_page | 1918 |
| container_title | Genome Research |
| container_volume | 18 |
| creator | Nagy, Ervin D Bennetzen, Jeffrey L |
| description | The Pc locus of sorghum (Sorghum bicolor) determines dominant sensitivity to a host-selective toxin produced by the fungal pathogen Periconia circinata. The Pc region was cloned by a map-based approach and found to contain three tandemly repeated genes with the structures of nucleotide binding site-leucine-rich repeat (NBS-LRR) disease resistance genes. Thirteen independent Pc-to-pc mutations were analyzed, and each was found to remove all or part of the central gene of the threesome. Hence, this central gene is Pc. Most Pc-to-pc mutations were associated with unequal recombination. Eight recombination events were localized to different sites in a 560-bp region within the approximately 3.7-kb NBS-LRR genes. Because any unequal recombination located within the flanking NBS-LRR genes would have removed Pc, the clustering of cross-over events within a 560-bp segment indicates that a site-directed recombination process exists that specifically targets unequal events to generate LRR diversity in NBS-LRR loci. |
| doi_str_mv | 10.1101/gr.078766.108 |
| format | Article |
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The Pc region was cloned by a map-based approach and found to contain three tandemly repeated genes with the structures of nucleotide binding site-leucine-rich repeat (NBS-LRR) disease resistance genes. Thirteen independent Pc-to-pc mutations were analyzed, and each was found to remove all or part of the central gene of the threesome. Hence, this central gene is Pc. Most Pc-to-pc mutations were associated with unequal recombination. Eight recombination events were localized to different sites in a 560-bp region within the approximately 3.7-kb NBS-LRR genes. 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The Pc region was cloned by a map-based approach and found to contain three tandemly repeated genes with the structures of nucleotide binding site-leucine-rich repeat (NBS-LRR) disease resistance genes. Thirteen independent Pc-to-pc mutations were analyzed, and each was found to remove all or part of the central gene of the threesome. Hence, this central gene is Pc. Most Pc-to-pc mutations were associated with unequal recombination. Eight recombination events were localized to different sites in a 560-bp region within the approximately 3.7-kb NBS-LRR genes. Because any unequal recombination located within the flanking NBS-LRR genes would have removed Pc, the clustering of cross-over events within a 560-bp segment indicates that a site-directed recombination process exists that specifically targets unequal events to generate LRR diversity in NBS-LRR loci.</description><subject>Ascomycota - pathogenicity</subject><subject>DNA, Plant - genetics</subject><subject>DNA, Plant - isolation & purification</subject><subject>Gene Library</subject><subject>Gene Rearrangement</subject><subject>Genes, Plant</subject><subject>Immunity, Innate</subject><subject>Letter</subject><subject>Multigene Family</subject><subject>Mutagenesis, Site-Directed</subject><subject>Periconia</subject><subject>Plant Diseases - genetics</subject><subject>Recombination, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>Sorghum</subject><subject>Sorghum - genetics</subject><subject>Sorghum - metabolism</subject><subject>Sorghum bicolor</subject><issn>1088-9051</issn><issn>1549-5469</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctLxDAQh4MoPlaPXiUnb12TtnldBBFfIOhBr4Zpkq6RbrMmqeB_b2QXH6cZMh9fZvghdEzJnFJCzxZxToQUnM8pkVton7JWVazlarv0RMpKEUb30EFKb4SQppVyF-1RKagiqtlHL4-QX8PCjdiEGKdV9mHEMFqcfHaV9dGZ7CwuJSw7P8J6njHg1QBjxtYnB8kVIPmUYTQOF5nDZphSdvEQ7fQwJHe0qTP0fH31dHlb3T_c3F1e3FempTxXTIhG9k2vOitrqbignRC27ZQC3oGpa9Zzy3ugDeVMir4moGqmAMCoTnS2maHztXc1dUtnjRtzhEGvol9C_NQBvP4_Gf2rXoQPXSwNE6oITjeCGN4nl7Je-mTcUI50YUq6JqQVLasLWK1BE0NK0fU_n1CivxPRi6jXiZQXWfiTv5v90psImi9beonB</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Nagy, Ervin D</creator><creator>Bennetzen, Jeffrey L</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>200812</creationdate><title>Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster</title><author>Nagy, Ervin D ; Bennetzen, Jeffrey L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-57738f3f9bd8289671b77d4b99a6bac225f6d6fa1316587f20a9259aaac9b7bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Ascomycota - pathogenicity</topic><topic>DNA, Plant - genetics</topic><topic>DNA, Plant - isolation & purification</topic><topic>Gene Library</topic><topic>Gene Rearrangement</topic><topic>Genes, Plant</topic><topic>Immunity, Innate</topic><topic>Letter</topic><topic>Multigene Family</topic><topic>Mutagenesis, Site-Directed</topic><topic>Periconia</topic><topic>Plant Diseases - genetics</topic><topic>Recombination, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>Sorghum</topic><topic>Sorghum - genetics</topic><topic>Sorghum - metabolism</topic><topic>Sorghum bicolor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagy, Ervin D</creatorcontrib><creatorcontrib>Bennetzen, Jeffrey L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genome Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagy, Ervin D</au><au>Bennetzen, Jeffrey L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster</atitle><jtitle>Genome Research</jtitle><addtitle>Genome Res</addtitle><date>2008-12</date><risdate>2008</risdate><volume>18</volume><issue>12</issue><spage>1918</spage><epage>1923</epage><pages>1918-1923</pages><issn>1088-9051</issn><eissn>1549-5469</eissn><eissn>1549-5477</eissn><abstract>The Pc locus of sorghum (Sorghum bicolor) determines dominant sensitivity to a host-selective toxin produced by the fungal pathogen Periconia circinata. The Pc region was cloned by a map-based approach and found to contain three tandemly repeated genes with the structures of nucleotide binding site-leucine-rich repeat (NBS-LRR) disease resistance genes. Thirteen independent Pc-to-pc mutations were analyzed, and each was found to remove all or part of the central gene of the threesome. Hence, this central gene is Pc. Most Pc-to-pc mutations were associated with unequal recombination. Eight recombination events were localized to different sites in a 560-bp region within the approximately 3.7-kb NBS-LRR genes. Because any unequal recombination located within the flanking NBS-LRR genes would have removed Pc, the clustering of cross-over events within a 560-bp segment indicates that a site-directed recombination process exists that specifically targets unequal events to generate LRR diversity in NBS-LRR loci.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>18719093</pmid><doi>10.1101/gr.078766.108</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genome Research, 2008-12, Vol.18 (12), p.1918-1923 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Ascomycota - pathogenicity DNA, Plant - genetics DNA, Plant - isolation & purification Gene Library Gene Rearrangement Genes, Plant Immunity, Innate Letter Multigene Family Mutagenesis, Site-Directed Periconia Plant Diseases - genetics Recombination, Genetic Sequence Analysis, DNA Sorghum Sorghum - genetics Sorghum - metabolism Sorghum bicolor |
| title | Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster |
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