Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats
Cannabinoid receptors 1 and 2 (CB1 and CB2) are G-protein coupled receptors that are expressed throughout the body. Cannabinoid receptors are expressed in the urinary bladder and may affect bladder function. The purpose of this study was twofold: to confirm the presence of cannabinoid receptors in t...
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| Veröffentlicht in: | Neuroscience letters 2008-11, Vol.445 (1), p.130-134 |
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| creator | Merriam, Fabiola Voznika Wang, Zun-yi Guerios, Simone Domit Bjorling, Dale E. |
| description | Cannabinoid receptors 1 and 2 (CB1 and CB2) are G-protein coupled receptors that are expressed throughout the body. Cannabinoid receptors are expressed in the urinary bladder and may affect bladder function. The purpose of this study was twofold: to confirm the presence of cannabinoid receptors in the bladder, the L6/S1 spinal cord, and dorsal root ganglia (DRG), and to determine the effects of acute and chronic bladder inflammation on expression of cannabinoid receptors. Acute or chronic bladder inflammation was induced in rats by intravesical administration of acrolein. Abundance of CB1 and CB2 protein and their respective mRNA was determined using immunoblotting and quantitative real-time PCR, respectively. We confirmed the presence of CB1 and CB2 receptor protein and mRNA in bladder, L6-S spinal cord, and DRG. Acute bladder inflammation induced increased expression of CB2, but not CB1, protein in the bladder detrusor. Chronic bladder inflammation increased expression of bladder CB2 protein and mRNA but not CB1 protein or mRNA. Expression of CB1 or CB2 in spinal cord or DRG was unaffected by acute or chronic bladder inflammation. CB1 and CB2 receptors are present in the bladder and its associated innervation, and CB2 receptors are up-regulated in bladder after acute or chronic inflammation. CB2 receptors may be a viable target for pharmacological treatment of bladder inflammation and associated pain. |
| doi_str_mv | 10.1016/j.neulet.2008.08.076 |
| format | Article |
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Cannabinoid receptors are expressed in the urinary bladder and may affect bladder function. The purpose of this study was twofold: to confirm the presence of cannabinoid receptors in the bladder, the L6/S1 spinal cord, and dorsal root ganglia (DRG), and to determine the effects of acute and chronic bladder inflammation on expression of cannabinoid receptors. Acute or chronic bladder inflammation was induced in rats by intravesical administration of acrolein. Abundance of CB1 and CB2 protein and their respective mRNA was determined using immunoblotting and quantitative real-time PCR, respectively. We confirmed the presence of CB1 and CB2 receptor protein and mRNA in bladder, L6-S spinal cord, and DRG. Acute bladder inflammation induced increased expression of CB2, but not CB1, protein in the bladder detrusor. Chronic bladder inflammation increased expression of bladder CB2 protein and mRNA but not CB1 protein or mRNA. Expression of CB1 or CB2 in spinal cord or DRG was unaffected by acute or chronic bladder inflammation. CB1 and CB2 receptors are present in the bladder and its associated innervation, and CB2 receptors are up-regulated in bladder after acute or chronic inflammation. CB2 receptors may be a viable target for pharmacological treatment of bladder inflammation and associated pain.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2008.08.076</identifier><identifier>PMID: 18778751</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Acrolein ; Animals ; Bacterial diseases ; Bacterial diseases of the urinary system ; Biological and medical sciences ; Cannabinoid receptors ; Cystitis ; Cystitis - chemically induced ; Cystitis - metabolism ; Cystitis - pathology ; Disease Models, Animal ; Female ; Fundamental and applied biological sciences. Psychology ; Ganglia, Spinal - metabolism ; Gene Expression Regulation - drug effects ; Human bacterial diseases ; Infectious diseases ; Medical sciences ; Rats ; Rats, Sprague-Dawley ; Receptor, Cannabinoid, CB1 - genetics ; Receptor, Cannabinoid, CB1 - metabolism ; Receptor, Cannabinoid, CB2 - genetics ; Receptor, Cannabinoid, CB2 - metabolism ; RNA, Messenger - metabolism ; Spinal Cord - metabolism ; Time Factors ; Urinary Bladder - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2008-11, Vol.445 (1), p.130-134</ispartof><rights>2008 Elsevier Ireland Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-5338d8161fe3c2ba8a0c28ac0a7cc75ec66c320e957ed7bbe1a09de4dc462443</citedby><cites>FETCH-LOGICAL-c588t-5338d8161fe3c2ba8a0c28ac0a7cc75ec66c320e957ed7bbe1a09de4dc462443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2008.08.076$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20797505$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18778751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Merriam, Fabiola Voznika</creatorcontrib><creatorcontrib>Wang, Zun-yi</creatorcontrib><creatorcontrib>Guerios, Simone Domit</creatorcontrib><creatorcontrib>Bjorling, Dale E.</creatorcontrib><title>Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Cannabinoid receptors 1 and 2 (CB1 and CB2) are G-protein coupled receptors that are expressed throughout the body. Cannabinoid receptors are expressed in the urinary bladder and may affect bladder function. The purpose of this study was twofold: to confirm the presence of cannabinoid receptors in the bladder, the L6/S1 spinal cord, and dorsal root ganglia (DRG), and to determine the effects of acute and chronic bladder inflammation on expression of cannabinoid receptors. Acute or chronic bladder inflammation was induced in rats by intravesical administration of acrolein. Abundance of CB1 and CB2 protein and their respective mRNA was determined using immunoblotting and quantitative real-time PCR, respectively. We confirmed the presence of CB1 and CB2 receptor protein and mRNA in bladder, L6-S spinal cord, and DRG. Acute bladder inflammation induced increased expression of CB2, but not CB1, protein in the bladder detrusor. Chronic bladder inflammation increased expression of bladder CB2 protein and mRNA but not CB1 protein or mRNA. Expression of CB1 or CB2 in spinal cord or DRG was unaffected by acute or chronic bladder inflammation. CB1 and CB2 receptors are present in the bladder and its associated innervation, and CB2 receptors are up-regulated in bladder after acute or chronic inflammation. CB2 receptors may be a viable target for pharmacological treatment of bladder inflammation and associated pain.</description><subject>Acrolein</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the urinary system</subject><subject>Biological and medical sciences</subject><subject>Cannabinoid receptors</subject><subject>Cystitis</subject><subject>Cystitis - chemically induced</subject><subject>Cystitis - metabolism</subject><subject>Cystitis - pathology</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor, Cannabinoid, CB1 - genetics</subject><subject>Receptor, Cannabinoid, CB1 - metabolism</subject><subject>Receptor, Cannabinoid, CB2 - genetics</subject><subject>Receptor, Cannabinoid, CB2 - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Spinal Cord - metabolism</subject><subject>Time Factors</subject><subject>Urinary Bladder - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo7uzqPxDpi956rKQ_kr4IMugqLHhZ8BiqK9Vuhp5kTHoW9t-bcYZdvQgFSZGn3qrUK8QbCWsJsv-wXQc-zLysFYBZH0P3z8RKGq1qPWj1XKyggbZuhhYuxGXOWwDoZNe-FBcF0kZ3ciV-bDAEHH2I3lWJifdLTJWqfK58oMSY2ZVbhXRYeH6oMLiK7lIMnnAuuQ_TjLvCjDM6x6mKU5Vwya_EiwnnzK_P55W4_fL5dvO1vvl-_W3z6aamzpil7prGOCN7OXFDakSDQMogAWoi3TH1PTUKeOg0Oz2OLBEGx62jtldt21yJjyfZ_WEsUxCHJeFs98nvMD3YiN7--xL8nf0Z763qBgVmKALvzwIp_jpwXuzOZ-J5xsDxkK2S0EL_p1N7AinFnBNPj00k2KMhdmtPhtijIfYYui9lb_8e8Kno7EAB3p0BzGWlU8JAPj9yCoqXHXRPP-WyzXvPyWbyHIidL7Yt1kX__0l-A2VVrbc</recordid><startdate>20081107</startdate><enddate>20081107</enddate><creator>Merriam, Fabiola Voznika</creator><creator>Wang, Zun-yi</creator><creator>Guerios, Simone Domit</creator><creator>Bjorling, Dale E.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>20081107</creationdate><title>Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats</title><author>Merriam, Fabiola Voznika ; Wang, Zun-yi ; Guerios, Simone Domit ; Bjorling, Dale E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-5338d8161fe3c2ba8a0c28ac0a7cc75ec66c320e957ed7bbe1a09de4dc462443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acrolein</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the urinary system</topic><topic>Biological and medical sciences</topic><topic>Cannabinoid receptors</topic><topic>Cystitis</topic><topic>Cystitis - chemically induced</topic><topic>Cystitis - metabolism</topic><topic>Cystitis - pathology</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor, Cannabinoid, CB1 - genetics</topic><topic>Receptor, Cannabinoid, CB1 - metabolism</topic><topic>Receptor, Cannabinoid, CB2 - genetics</topic><topic>Receptor, Cannabinoid, CB2 - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Spinal Cord - metabolism</topic><topic>Time Factors</topic><topic>Urinary Bladder - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Merriam, Fabiola Voznika</creatorcontrib><creatorcontrib>Wang, Zun-yi</creatorcontrib><creatorcontrib>Guerios, Simone Domit</creatorcontrib><creatorcontrib>Bjorling, Dale E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Merriam, Fabiola Voznika</au><au>Wang, Zun-yi</au><au>Guerios, Simone Domit</au><au>Bjorling, Dale E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2008-11-07</date><risdate>2008</risdate><volume>445</volume><issue>1</issue><spage>130</spage><epage>134</epage><pages>130-134</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Cannabinoid receptors 1 and 2 (CB1 and CB2) are G-protein coupled receptors that are expressed throughout the body. Cannabinoid receptors are expressed in the urinary bladder and may affect bladder function. The purpose of this study was twofold: to confirm the presence of cannabinoid receptors in the bladder, the L6/S1 spinal cord, and dorsal root ganglia (DRG), and to determine the effects of acute and chronic bladder inflammation on expression of cannabinoid receptors. Acute or chronic bladder inflammation was induced in rats by intravesical administration of acrolein. Abundance of CB1 and CB2 protein and their respective mRNA was determined using immunoblotting and quantitative real-time PCR, respectively. We confirmed the presence of CB1 and CB2 receptor protein and mRNA in bladder, L6-S spinal cord, and DRG. Acute bladder inflammation induced increased expression of CB2, but not CB1, protein in the bladder detrusor. Chronic bladder inflammation increased expression of bladder CB2 protein and mRNA but not CB1 protein or mRNA. Expression of CB1 or CB2 in spinal cord or DRG was unaffected by acute or chronic bladder inflammation. CB1 and CB2 receptors are present in the bladder and its associated innervation, and CB2 receptors are up-regulated in bladder after acute or chronic inflammation. CB2 receptors may be a viable target for pharmacological treatment of bladder inflammation and associated pain.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>18778751</pmid><doi>10.1016/j.neulet.2008.08.076</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neuroscience letters, 2008-11, Vol.445 (1), p.130-134 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acrolein Animals Bacterial diseases Bacterial diseases of the urinary system Biological and medical sciences Cannabinoid receptors Cystitis Cystitis - chemically induced Cystitis - metabolism Cystitis - pathology Disease Models, Animal Female Fundamental and applied biological sciences. Psychology Ganglia, Spinal - metabolism Gene Expression Regulation - drug effects Human bacterial diseases Infectious diseases Medical sciences Rats Rats, Sprague-Dawley Receptor, Cannabinoid, CB1 - genetics Receptor, Cannabinoid, CB1 - metabolism Receptor, Cannabinoid, CB2 - genetics Receptor, Cannabinoid, CB2 - metabolism RNA, Messenger - metabolism Spinal Cord - metabolism Time Factors Urinary Bladder - metabolism Vertebrates: nervous system and sense organs |
| title | Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats |
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