Molecular Marker Systems for Oenothera Genetics
The genus Oenothera has an outstanding scientific tradition. It has been a model for studying aspects of chromosome evolution and speciation, including the impact of plastid nuclear co-evolution. A large collection of strains analyzed during a century of experimental work and unique genetic possibil...
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| Veröffentlicht in: | Genetics (Austin) 2008-11, Vol.180 (3), p.1289-1306 |
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| creator | Rauwolf, Uwe Golczyk, Hieronim Meurer, Jorg Herrmann, Reinhold G Greiner, Stephan |
| description | The genus Oenothera has an outstanding scientific tradition. It has been a model for studying aspects of chromosome evolution and speciation, including the impact of plastid nuclear co-evolution. A large collection of strains analyzed during a century of experimental work and unique genetic possibilities allow the exchange of genetically definable plastids, individual or multiple chromosomes, and/or entire haploid genomes (Renner complexes) between species. However, molecular genetic approaches for the genus are largely lacking. In this study, we describe the development of efficient PCR-based marker systems for both the nuclear genome and the plastome. They allow distinguishing individual chromosomes, Renner complexes, plastomes, and subplastomes. We demonstrate their application by monitoring interspecific exchanges of genomes, chromosome pairs, and/or plastids during crossing programs, e.g., to produce plastome-genome incompatible hybrids. Using an appropriate partial permanent translocation heterozygous hybrid, linkage group 7 of the molecular map could be assigned to chromosome 9.8 of the classical Oenothera map. Finally, we provide the first direct molecular evidence that homologous recombination and free segregation of chromosomes in permanent translocation heterozygous strains is suppressed. |
| doi_str_mv | 10.1534/genetics.108.091249 |
| format | Article |
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It has been a model for studying aspects of chromosome evolution and speciation, including the impact of plastid nuclear co-evolution. A large collection of strains analyzed during a century of experimental work and unique genetic possibilities allow the exchange of genetically definable plastids, individual or multiple chromosomes, and/or entire haploid genomes (Renner complexes) between species. However, molecular genetic approaches for the genus are largely lacking. In this study, we describe the development of efficient PCR-based marker systems for both the nuclear genome and the plastome. They allow distinguishing individual chromosomes, Renner complexes, plastomes, and subplastomes. We demonstrate their application by monitoring interspecific exchanges of genomes, chromosome pairs, and/or plastids during crossing programs, e.g., to produce plastome-genome incompatible hybrids. Using an appropriate partial permanent translocation heterozygous hybrid, linkage group 7 of the molecular map could be assigned to chromosome 9.8 of the classical Oenothera map. Finally, we provide the first direct molecular evidence that homologous recombination and free segregation of chromosomes in permanent translocation heterozygous strains is suppressed.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1534/genetics.108.091249</identifier><identifier>PMID: 18791241</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>amplified fragment length polymorphism ; Cell Nucleus - genetics ; chloroplast DNA ; Chromosome Mapping ; chromosome translocation ; Chromosomes, Plant - genetics ; Comparative studies ; DNA, Plant - genetics ; Food supply ; Genetic engineering ; genetic markers ; Genetic Markers - genetics ; Genome, Plant - genetics ; Genome, Plastid - genetics ; Genomics ; Genotype ; homologous recombination ; Industrial production ; interspecific hybridization ; Investigations ; linkage groups ; molecular genetics ; Molecular Sequence Data ; nuclear genome ; nucleotide sequences ; Oenothera ; Oenothera - genetics ; Oenothera - growth & development ; plant genetics ; Plastids - genetics ; polymerase chain reaction ; Recombination, Genetic ; Renner complexes</subject><ispartof>Genetics (Austin), 2008-11, Vol.180 (3), p.1289-1306</ispartof><rights>Copyright Genetics Society of America Nov 2008</rights><rights>Copyright © 2008 by the Genetics Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-c3e02ddb263a37a77a939fe797d6548b9331b7e8ebf950284294a14f55b801303</citedby><cites>FETCH-LOGICAL-c486t-c3e02ddb263a37a77a939fe797d6548b9331b7e8ebf950284294a14f55b801303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18791241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rauwolf, Uwe</creatorcontrib><creatorcontrib>Golczyk, Hieronim</creatorcontrib><creatorcontrib>Meurer, Jorg</creatorcontrib><creatorcontrib>Herrmann, Reinhold G</creatorcontrib><creatorcontrib>Greiner, Stephan</creatorcontrib><title>Molecular Marker Systems for Oenothera Genetics</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The genus Oenothera has an outstanding scientific tradition. It has been a model for studying aspects of chromosome evolution and speciation, including the impact of plastid nuclear co-evolution. A large collection of strains analyzed during a century of experimental work and unique genetic possibilities allow the exchange of genetically definable plastids, individual or multiple chromosomes, and/or entire haploid genomes (Renner complexes) between species. However, molecular genetic approaches for the genus are largely lacking. In this study, we describe the development of efficient PCR-based marker systems for both the nuclear genome and the plastome. They allow distinguishing individual chromosomes, Renner complexes, plastomes, and subplastomes. We demonstrate their application by monitoring interspecific exchanges of genomes, chromosome pairs, and/or plastids during crossing programs, e.g., to produce plastome-genome incompatible hybrids. Using an appropriate partial permanent translocation heterozygous hybrid, linkage group 7 of the molecular map could be assigned to chromosome 9.8 of the classical Oenothera map. Finally, we provide the first direct molecular evidence that homologous recombination and free segregation of chromosomes in permanent translocation heterozygous strains is suppressed.</description><subject>amplified fragment length polymorphism</subject><subject>Cell Nucleus - genetics</subject><subject>chloroplast DNA</subject><subject>Chromosome Mapping</subject><subject>chromosome translocation</subject><subject>Chromosomes, Plant - genetics</subject><subject>Comparative studies</subject><subject>DNA, Plant - genetics</subject><subject>Food supply</subject><subject>Genetic engineering</subject><subject>genetic markers</subject><subject>Genetic Markers - genetics</subject><subject>Genome, Plant - genetics</subject><subject>Genome, Plastid - genetics</subject><subject>Genomics</subject><subject>Genotype</subject><subject>homologous recombination</subject><subject>Industrial production</subject><subject>interspecific hybridization</subject><subject>Investigations</subject><subject>linkage groups</subject><subject>molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nuclear genome</subject><subject>nucleotide sequences</subject><subject>Oenothera</subject><subject>Oenothera - genetics</subject><subject>Oenothera - growth & development</subject><subject>plant genetics</subject><subject>Plastids - genetics</subject><subject>polymerase chain reaction</subject><subject>Recombination, Genetic</subject><subject>Renner complexes</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1v1DAQhi0EokvhFyBBxAFO2Xr8EdsXJFRBQWrVQ-l55GQnuylJXOyEVf89XmX5PI0088w7Hy9jL4GvQUt1tqWRpq5Ja-B2zR0I5R6xFTglS1FJeMxWnENVVkbCCXuW0h3nvHLaPmUnYM2BhxU7uwo9NXPvY3Hl4zeKxc1DmmhIRRticU1jmHYUfXFxHPacPWl9n-jFMZ6y208fv55_Li-vL76cf7gsG2WrqWwkcbHZ1HkRL403xjvpWjLObCqtbO2khNqQpbp1mgurhFMeVKt1bTlILk_Z-0X3fq4H2jQ0TtH3eB-7wccHDL7Dfytjt8Nt-IFCW3BSZ4G3R4EYvs-UJhy61FDf-5HCnLByJoNcZPDNf-BdmOOYj0MBCoQ2QmVILlATQ0qR2t-bAMeDG_jLjZywuLiRu179fcSfnuP7M_BuAXbddrfvImEafN9nHHC_34PlKBGEPUi9XsjWB_Tb2CW8vRGHV4GurNJK_gQWIZ2m</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Rauwolf, Uwe</creator><creator>Golczyk, Hieronim</creator><creator>Meurer, Jorg</creator><creator>Herrmann, Reinhold G</creator><creator>Greiner, Stephan</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20081101</creationdate><title>Molecular Marker Systems for Oenothera Genetics</title><author>Rauwolf, Uwe ; Golczyk, Hieronim ; Meurer, Jorg ; Herrmann, Reinhold G ; Greiner, Stephan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-c3e02ddb263a37a77a939fe797d6548b9331b7e8ebf950284294a14f55b801303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>amplified fragment length polymorphism</topic><topic>Cell Nucleus - 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It has been a model for studying aspects of chromosome evolution and speciation, including the impact of plastid nuclear co-evolution. A large collection of strains analyzed during a century of experimental work and unique genetic possibilities allow the exchange of genetically definable plastids, individual or multiple chromosomes, and/or entire haploid genomes (Renner complexes) between species. However, molecular genetic approaches for the genus are largely lacking. In this study, we describe the development of efficient PCR-based marker systems for both the nuclear genome and the plastome. They allow distinguishing individual chromosomes, Renner complexes, plastomes, and subplastomes. We demonstrate their application by monitoring interspecific exchanges of genomes, chromosome pairs, and/or plastids during crossing programs, e.g., to produce plastome-genome incompatible hybrids. Using an appropriate partial permanent translocation heterozygous hybrid, linkage group 7 of the molecular map could be assigned to chromosome 9.8 of the classical Oenothera map. Finally, we provide the first direct molecular evidence that homologous recombination and free segregation of chromosomes in permanent translocation heterozygous strains is suppressed.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>18791241</pmid><doi>10.1534/genetics.108.091249</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genetics (Austin), 2008-11, Vol.180 (3), p.1289-1306 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | amplified fragment length polymorphism Cell Nucleus - genetics chloroplast DNA Chromosome Mapping chromosome translocation Chromosomes, Plant - genetics Comparative studies DNA, Plant - genetics Food supply Genetic engineering genetic markers Genetic Markers - genetics Genome, Plant - genetics Genome, Plastid - genetics Genomics Genotype homologous recombination Industrial production interspecific hybridization Investigations linkage groups molecular genetics Molecular Sequence Data nuclear genome nucleotide sequences Oenothera Oenothera - genetics Oenothera - growth & development plant genetics Plastids - genetics polymerase chain reaction Recombination, Genetic Renner complexes |
| title | Molecular Marker Systems for Oenothera Genetics |
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