A monovalent streptavidin with a single femtomolar biotin binding site

Streptavidin and avidin are used ubiquitously because of the remarkable affinity of their biotin binding, but they are tetramers, which disrupts many of their applications. Making either protein monomeric reduces affinity by at least 10 4 -fold because part of the binding site comes from a neighbori...

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Veröffentlicht in:	Nature methods 2006-04, Vol.3 (4), p.267-273
Hauptverfasser:	Howarth, Mark, Chinnapen, Daniel J-F, Gerrow, Kimberly, Dorrestein, Pieter C, Grandy, Melanie R, Kelleher, Neil L, El-Husseini, Alaa, Ting, Alice Y
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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