Identification of novel neuroendocrine-specific tumour genes
Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling,...
Gespeichert in:
| Veröffentlicht in: | British journal of cancer 2008-10, Vol.99 (8), p.1330-1339 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1339 |
|---|---|
| container_issue | 8 |
| container_start_page | 1330 |
| container_title | British journal of cancer |
| container_volume | 99 |
| creator | Hofsli, E Wheeler, T E Langaas, M Lægreid, A Thommesen, L |
| description | Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling, sought to identify genes that are either up- or downregulated in NE as compared with non-NE tumour cells. A panel of six NET and four non-NET cell lines were examined, and out of 12 743 genes examined, we studied in detail the 200 most significantly differentially expressed genes in the comparison. In addition to potential new diagnostic markers (
NEFM
,
CLDN4
,
PEROX2
), the results point to genes that may be involved in the tumorigenesis (
BEX1
,
TMEPAI
,
FOSL1
,
RAB32
), and in the processes of invasion, progression and metastasis (
MME
,
STAT3
,
DCBLD2
) of NETs. Verification by real time qRT–PCR showed a high degree of consistency to the microarray results. Furthermore, the protein expression of some of the genes were examined. The results of our study has opened a window to new areas of research, by uncovering new candidate genes and proteins to be further investigated in the search for new prognostic, predictive, and therapeutic markers in NETs. |
| doi_str_mv | 10.1038/sj.bjc.6604565 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2570516</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69662303</sourcerecordid><originalsourceid>FETCH-LOGICAL-c516t-97da7497dcf301b50b3aa5bf0a068cae9a85ab210215486a4f319614159014133</originalsourceid><addsrcrecordid>eNqFkc1r3DAQxUVpabZJrzkGU2hv3owkS5YgBEroRyDQS3MWY628kfFKG8kO9L-vwpqkDZReRoj56c3TPEJOKawpcHWeh3U32LWU0AgpXpEVFZzVVLH2NVkBQFuDZnBE3uU8lKsG1b4lR1QVQDFYkYvrjQuT773FycdQxb4K8cGNVXBzii5sok0-uDrvnX2kqmnexTlVWxdcPiFvehyze7-cx-T265efV9_rmx_frq8-39RWUDnVut1g25Rqew60E9BxRNH1gCCVRadRCewYBUZFoyQ2Pada0oYKDaVyfkwuD7r7udu5jS2OE45mn_wO0y8T0Zu_O8HfmW18MEy0UCwUgU-LQIr3s8uT2fls3ThicHHORmopGQf-X5BqBdAwVsAPL8ChrCWULZgiBFQorgu0PkA2xZyT658sUzCP8Zk8mBKfWeIrD87-_OgzvuRVgI8LgNni2CcM1ucnjoHSDWVN4c4PXC6tsHXp2d4_Rv8GBrKy-A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>230015839</pqid></control><display><type>article</type><title>Identification of novel neuroendocrine-specific tumour genes</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Nature Journals Online</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Hofsli, E ; Wheeler, T E ; Langaas, M ; Lægreid, A ; Thommesen, L</creator><creatorcontrib>Hofsli, E ; Wheeler, T E ; Langaas, M ; Lægreid, A ; Thommesen, L</creatorcontrib><description>Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling, sought to identify genes that are either up- or downregulated in NE as compared with non-NE tumour cells. A panel of six NET and four non-NET cell lines were examined, and out of 12 743 genes examined, we studied in detail the 200 most significantly differentially expressed genes in the comparison. In addition to potential new diagnostic markers (
NEFM
,
CLDN4
,
PEROX2
), the results point to genes that may be involved in the tumorigenesis (
BEX1
,
TMEPAI
,
FOSL1
,
RAB32
), and in the processes of invasion, progression and metastasis (
MME
,
STAT3
,
DCBLD2
) of NETs. Verification by real time qRT–PCR showed a high degree of consistency to the microarray results. Furthermore, the protein expression of some of the genes were examined. The results of our study has opened a window to new areas of research, by uncovering new candidate genes and proteins to be further investigated in the search for new prognostic, predictive, and therapeutic markers in NETs.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/sj.bjc.6604565</identifier><identifier>PMID: 18827820</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Arrays ; Biological and medical sciences ; Biology ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Cancer Research ; Cell Line, Tumor ; Drug Resistance ; Epidemiology ; Gene Expression ; Gene Expression Profiling ; Genetics and Genomics ; Genomics ; Hospitals ; Humans ; Immunohistochemistry ; Medical research ; Medical sciences ; Molecular Medicine ; Neuroendocrine tumors ; Neuroendocrine Tumors - genetics ; Oligonucleotide Array Sequence Analysis ; Oncology ; Protein Array Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Tumorigenesis ; Tumors</subject><ispartof>British journal of cancer, 2008-10, Vol.99 (8), p.1330-1339</ispartof><rights>The Author(s) 2008</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Oct 21, 2008</rights><rights>Copyright © 2008 Cancer Research UK 2008 Cancer Research UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-97da7497dcf301b50b3aa5bf0a068cae9a85ab210215486a4f319614159014133</citedby><cites>FETCH-LOGICAL-c516t-97da7497dcf301b50b3aa5bf0a068cae9a85ab210215486a4f319614159014133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570516/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570516/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20894124$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18827820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hofsli, E</creatorcontrib><creatorcontrib>Wheeler, T E</creatorcontrib><creatorcontrib>Langaas, M</creatorcontrib><creatorcontrib>Lægreid, A</creatorcontrib><creatorcontrib>Thommesen, L</creatorcontrib><title>Identification of novel neuroendocrine-specific tumour genes</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling, sought to identify genes that are either up- or downregulated in NE as compared with non-NE tumour cells. A panel of six NET and four non-NET cell lines were examined, and out of 12 743 genes examined, we studied in detail the 200 most significantly differentially expressed genes in the comparison. In addition to potential new diagnostic markers (
NEFM
,
CLDN4
,
PEROX2
), the results point to genes that may be involved in the tumorigenesis (
BEX1
,
TMEPAI
,
FOSL1
,
RAB32
), and in the processes of invasion, progression and metastasis (
MME
,
STAT3
,
DCBLD2
) of NETs. Verification by real time qRT–PCR showed a high degree of consistency to the microarray results. Furthermore, the protein expression of some of the genes were examined. The results of our study has opened a window to new areas of research, by uncovering new candidate genes and proteins to be further investigated in the search for new prognostic, predictive, and therapeutic markers in NETs.</description><subject>Arrays</subject><subject>Biological and medical sciences</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Cancer Research</subject><subject>Cell Line, Tumor</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Genetics and Genomics</subject><subject>Genomics</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Molecular Medicine</subject><subject>Neuroendocrine tumors</subject><subject>Neuroendocrine Tumors - genetics</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Oncology</subject><subject>Protein Array Analysis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1r3DAQxUVpabZJrzkGU2hv3owkS5YgBEroRyDQS3MWY628kfFKG8kO9L-vwpqkDZReRoj56c3TPEJOKawpcHWeh3U32LWU0AgpXpEVFZzVVLH2NVkBQFuDZnBE3uU8lKsG1b4lR1QVQDFYkYvrjQuT773FycdQxb4K8cGNVXBzii5sok0-uDrvnX2kqmnexTlVWxdcPiFvehyze7-cx-T265efV9_rmx_frq8-39RWUDnVut1g25Rqew60E9BxRNH1gCCVRadRCewYBUZFoyQ2Pada0oYKDaVyfkwuD7r7udu5jS2OE45mn_wO0y8T0Zu_O8HfmW18MEy0UCwUgU-LQIr3s8uT2fls3ThicHHORmopGQf-X5BqBdAwVsAPL8ChrCWULZgiBFQorgu0PkA2xZyT658sUzCP8Zk8mBKfWeIrD87-_OgzvuRVgI8LgNni2CcM1ucnjoHSDWVN4c4PXC6tsHXp2d4_Rv8GBrKy-A</recordid><startdate>20081021</startdate><enddate>20081021</enddate><creator>Hofsli, E</creator><creator>Wheeler, T E</creator><creator>Langaas, M</creator><creator>Lægreid, A</creator><creator>Thommesen, L</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20081021</creationdate><title>Identification of novel neuroendocrine-specific tumour genes</title><author>Hofsli, E ; Wheeler, T E ; Langaas, M ; Lægreid, A ; Thommesen, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-97da7497dcf301b50b3aa5bf0a068cae9a85ab210215486a4f319614159014133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Arrays</topic><topic>Biological and medical sciences</topic><topic>Biology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Western</topic><topic>Cancer Research</topic><topic>Cell Line, Tumor</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Genetics and Genomics</topic><topic>Genomics</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Molecular Medicine</topic><topic>Neuroendocrine tumors</topic><topic>Neuroendocrine Tumors - genetics</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Oncology</topic><topic>Protein Array Analysis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hofsli, E</creatorcontrib><creatorcontrib>Wheeler, T E</creatorcontrib><creatorcontrib>Langaas, M</creatorcontrib><creatorcontrib>Lægreid, A</creatorcontrib><creatorcontrib>Thommesen, L</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hofsli, E</au><au>Wheeler, T E</au><au>Langaas, M</au><au>Lægreid, A</au><au>Thommesen, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of novel neuroendocrine-specific tumour genes</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2008-10-21</date><risdate>2008</risdate><volume>99</volume><issue>8</issue><spage>1330</spage><epage>1339</epage><pages>1330-1339</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling, sought to identify genes that are either up- or downregulated in NE as compared with non-NE tumour cells. A panel of six NET and four non-NET cell lines were examined, and out of 12 743 genes examined, we studied in detail the 200 most significantly differentially expressed genes in the comparison. In addition to potential new diagnostic markers (
NEFM
,
CLDN4
,
PEROX2
), the results point to genes that may be involved in the tumorigenesis (
BEX1
,
TMEPAI
,
FOSL1
,
RAB32
), and in the processes of invasion, progression and metastasis (
MME
,
STAT3
,
DCBLD2
) of NETs. Verification by real time qRT–PCR showed a high degree of consistency to the microarray results. Furthermore, the protein expression of some of the genes were examined. The results of our study has opened a window to new areas of research, by uncovering new candidate genes and proteins to be further investigated in the search for new prognostic, predictive, and therapeutic markers in NETs.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>18827820</pmid><doi>10.1038/sj.bjc.6604565</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-0920 |
| ispartof | British journal of cancer, 2008-10, Vol.99 (8), p.1330-1339 |
| issn | 0007-0920 1532-1827 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2570516 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Arrays Biological and medical sciences Biology Biomedical and Life Sciences Biomedicine Blotting, Western Cancer Research Cell Line, Tumor Drug Resistance Epidemiology Gene Expression Gene Expression Profiling Genetics and Genomics Genomics Hospitals Humans Immunohistochemistry Medical research Medical sciences Molecular Medicine Neuroendocrine tumors Neuroendocrine Tumors - genetics Oligonucleotide Array Sequence Analysis Oncology Protein Array Analysis Reverse Transcriptase Polymerase Chain Reaction Tumorigenesis Tumors |
| title | Identification of novel neuroendocrine-specific tumour genes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T00%3A09%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20novel%20neuroendocrine-specific%20tumour%20genes&rft.jtitle=British%20journal%20of%20cancer&rft.au=Hofsli,%20E&rft.date=2008-10-21&rft.volume=99&rft.issue=8&rft.spage=1330&rft.epage=1339&rft.pages=1330-1339&rft.issn=0007-0920&rft.eissn=1532-1827&rft.coden=BJCAAI&rft_id=info:doi/10.1038/sj.bjc.6604565&rft_dat=%3Cproquest_pubme%3E69662303%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=230015839&rft_id=info:pmid/18827820&rfr_iscdi=true |