Engineered Recombinant Single-Chain Fragment Variable Antibody for Immunosensors

A recombinant single-chain fragment variable (scFv) antibody (designated A10B) was engineered to contain two histidines within the linker peptide used to join the scFv heavy and light chains. A piezoimmunosensor using the scFv was successfully developed. A10B scFv bound to the gold piezoimmunosensor...

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Veröffentlicht in:	Analytical chemistry (Washington) 2005-11, Vol.77 (21), p.6834-6842
Hauptverfasser:	Shen, Zhihong, Mernaugh, Raymond L, Yan, Heping, Yu, Lei, Zhang, Ying, Zeng, Xiangqun
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical chemistry Binding sites Biosensing Techniques - instrumentation Biosensing Techniques - methods Chemistry Exact sciences and technology General, instrumentation Genetic recombination Gold - chemistry Histidine - chemistry Immunoglobulin Fragments - chemistry Immunoglobulin G - analysis Immunoglobulin Variable Region - chemistry Peptides Protein Binding Protein Engineering - methods Recombinant Proteins - chemistry Sensitivity and Specificity
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