Expression and function of Nkx6.3 in vertebrate hindbrain
Abstract Homeodomain transcription factors serve important functions in organogenesis and tissue differentiation, particularly with respect to the positional identity of individual cells. The Nkx6 subfamily controls tissue differentiation in the developing central nervous system where they function...
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| Veröffentlicht in: | Brain research 2008-07, Vol.1222, p.42-50 |
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| creator | Hafler, Brian P Choi, Michael Y Shivdasani, Ramesh A Rowitch, David H |
| description | Abstract Homeodomain transcription factors serve important functions in organogenesis and tissue differentiation, particularly with respect to the positional identity of individual cells. The Nkx6 subfamily controls tissue differentiation in the developing central nervous system where they function as transcriptional repressor proteins. Recent work indicates that Nkx6.3 is expressed in hindbrain V2 interneurons that co-express Nkx6.1, suggesting the possibility of functional redundancy. Here, we report that Nkx6.3 expression is specific to Chx10+ V2a interneurons but not to Gata3+ V2b interneurons of the hindbrain, and that Nkx6.3 expression appears to mark cells of the prospective medullary reticular formation. Molecular analysis of Nkx6.3 null embryonic mouse hindbrain did not reveal detectable defects in progenitor markers, motor neuron or V2 interneuron sub-types. Forced expression of Nkx6.3 and Nkx6.1 promote V2 interneuron differentiation in the developing chick hindbrain. These findings indicate Nkx6.3 function is dispensable for CNS development and lead to the proposal that absence of overt defects is due to functional compensation from a related homeodomain transcription factor. |
| doi_str_mv | 10.1016/j.brainres.2008.04.072 |
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The Nkx6 subfamily controls tissue differentiation in the developing central nervous system where they function as transcriptional repressor proteins. Recent work indicates that Nkx6.3 is expressed in hindbrain V2 interneurons that co-express Nkx6.1, suggesting the possibility of functional redundancy. Here, we report that Nkx6.3 expression is specific to Chx10+ V2a interneurons but not to Gata3+ V2b interneurons of the hindbrain, and that Nkx6.3 expression appears to mark cells of the prospective medullary reticular formation. Molecular analysis of Nkx6.3 null embryonic mouse hindbrain did not reveal detectable defects in progenitor markers, motor neuron or V2 interneuron sub-types. Forced expression of Nkx6.3 and Nkx6.1 promote V2 interneuron differentiation in the developing chick hindbrain. These findings indicate Nkx6.3 function is dispensable for CNS development and lead to the proposal that absence of overt defects is due to functional compensation from a related homeodomain transcription factor.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2008.04.072</identifier><identifier>PMID: 18586225</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Central nervous system ; CNS development ; Electroporation - methods ; Embryo, Mammalian ; Eye Proteins - genetics ; Eye Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression - physiology ; Gene Expression Regulation, Developmental - genetics ; Homeobox transcription factor ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Homeodomain Proteins - physiology ; Interneurons - metabolism ; LIM-Homeodomain Proteins ; Medullary reticular formation ; Mice ; Mice, Inbred C57BL ; Mice, Mutant Strains ; Mutation ; Neurology ; Nkx6.1 ; Nkx6.3 ; Paired Box Transcription Factors - genetics ; Paired Box Transcription Factors - metabolism ; PAX6 Transcription Factor ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Rhombencephalon - cytology ; Rhombencephalon - embryology ; Rhombencephalon - metabolism ; Rhombomere ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription Factors - physiology ; V2 interneuron ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 2008-07, Vol.1222, p.42-50</ispartof><rights>Elsevier B.V.</rights><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c651t-7bda40010c0af2a0750d763a67546ced09e346709dd832a30af5ed5f192b56993</citedby><cites>FETCH-LOGICAL-c651t-7bda40010c0af2a0750d763a67546ced09e346709dd832a30af5ed5f192b56993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006899308010330$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20552147$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18586225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hafler, Brian P</creatorcontrib><creatorcontrib>Choi, Michael Y</creatorcontrib><creatorcontrib>Shivdasani, Ramesh A</creatorcontrib><creatorcontrib>Rowitch, David H</creatorcontrib><title>Expression and function of Nkx6.3 in vertebrate hindbrain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract Homeodomain transcription factors serve important functions in organogenesis and tissue differentiation, particularly with respect to the positional identity of individual cells. The Nkx6 subfamily controls tissue differentiation in the developing central nervous system where they function as transcriptional repressor proteins. Recent work indicates that Nkx6.3 is expressed in hindbrain V2 interneurons that co-express Nkx6.1, suggesting the possibility of functional redundancy. Here, we report that Nkx6.3 expression is specific to Chx10+ V2a interneurons but not to Gata3+ V2b interneurons of the hindbrain, and that Nkx6.3 expression appears to mark cells of the prospective medullary reticular formation. Molecular analysis of Nkx6.3 null embryonic mouse hindbrain did not reveal detectable defects in progenitor markers, motor neuron or V2 interneuron sub-types. Forced expression of Nkx6.3 and Nkx6.1 promote V2 interneuron differentiation in the developing chick hindbrain. These findings indicate Nkx6.3 function is dispensable for CNS development and lead to the proposal that absence of overt defects is due to functional compensation from a related homeodomain transcription factor.</description><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>CNS development</subject><subject>Electroporation - methods</subject><subject>Embryo, Mammalian</subject><subject>Eye Proteins - genetics</subject><subject>Eye Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - physiology</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Homeobox transcription factor</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Homeodomain Proteins - physiology</subject><subject>Interneurons - metabolism</subject><subject>LIM-Homeodomain Proteins</subject><subject>Medullary reticular formation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Mutant Strains</subject><subject>Mutation</subject><subject>Neurology</subject><subject>Nkx6.1</subject><subject>Nkx6.3</subject><subject>Paired Box Transcription Factors - genetics</subject><subject>Paired Box Transcription Factors - metabolism</subject><subject>PAX6 Transcription Factor</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Rhombencephalon - cytology</subject><subject>Rhombencephalon - embryology</subject><subject>Rhombencephalon - metabolism</subject><subject>Rhombomere</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors - physiology</subject><subject>V2 interneuron</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi0EokvhL1S5wC1hbMd2fKlAVfmQKjgAZ8trT6i3WWexk1X773HYpXxcerJGfuadj3cIOaPQUKDy9aZZJxtiwtwwgK6BtgHFHpEV7RSrJWvhMVkBgKw7rfkJeZbzpoSca3hKTmgnOsmYWBF9ebsrIjmMsbLRV_0c3bQEY199urmVDa9CrPaYJiwFJ6yuQ_S_Sj8nT3o7ZHxxfE_Jt3eXXy8-1Fef33-8eHtVOynoVKu1ty0ABQe2ZxaUAK8kt1KJVjr0oJG3UoH2vuPM8kIJ9KKnmq2FLL2fkvOD7m5eb9E7jFOyg9mlsLXpzow2mH9_Yrg238e9YUIIrWgReHUUSOOPGfNktiE7HAYbcZyzkZpTrYA9CDLohNJtV0B5AF0ac07Y33dDwSz2mI35bY9Z7DHQmmJPSTz7e5Y_aUc_CvDyCNjs7NAnG13I9xwDIRhtVeHeHDgsm98HTCa7gLHsMyR0k_FjeLiX8_8k3BBiKFVv8A7zZpxTLL4aajIzYL4sx7TcEnTFTM6B_wSQgcXr</recordid><startdate>20080730</startdate><enddate>20080730</enddate><creator>Hafler, Brian P</creator><creator>Choi, Michael Y</creator><creator>Shivdasani, Ramesh A</creator><creator>Rowitch, David H</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080730</creationdate><title>Expression and function of Nkx6.3 in vertebrate hindbrain</title><author>Hafler, Brian P ; Choi, Michael Y ; Shivdasani, Ramesh A ; Rowitch, David H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c651t-7bda40010c0af2a0750d763a67546ced09e346709dd832a30af5ed5f192b56993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>CNS development</topic><topic>Electroporation - methods</topic><topic>Embryo, Mammalian</topic><topic>Eye Proteins - genetics</topic><topic>Eye Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - physiology</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Homeobox transcription factor</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Homeodomain Proteins - physiology</topic><topic>Interneurons - metabolism</topic><topic>LIM-Homeodomain Proteins</topic><topic>Medullary reticular formation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Mutant Strains</topic><topic>Mutation</topic><topic>Neurology</topic><topic>Nkx6.1</topic><topic>Nkx6.3</topic><topic>Paired Box Transcription Factors - genetics</topic><topic>Paired Box Transcription Factors - metabolism</topic><topic>PAX6 Transcription Factor</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Rhombencephalon - cytology</topic><topic>Rhombencephalon - embryology</topic><topic>Rhombencephalon - metabolism</topic><topic>Rhombomere</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors - physiology</topic><topic>V2 interneuron</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hafler, Brian P</creatorcontrib><creatorcontrib>Choi, Michael Y</creatorcontrib><creatorcontrib>Shivdasani, Ramesh A</creatorcontrib><creatorcontrib>Rowitch, David H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hafler, Brian P</au><au>Choi, Michael Y</au><au>Shivdasani, Ramesh A</au><au>Rowitch, David H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and function of Nkx6.3 in vertebrate hindbrain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2008-07-30</date><risdate>2008</risdate><volume>1222</volume><spage>42</spage><epage>50</epage><pages>42-50</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Abstract Homeodomain transcription factors serve important functions in organogenesis and tissue differentiation, particularly with respect to the positional identity of individual cells. The Nkx6 subfamily controls tissue differentiation in the developing central nervous system where they function as transcriptional repressor proteins. Recent work indicates that Nkx6.3 is expressed in hindbrain V2 interneurons that co-express Nkx6.1, suggesting the possibility of functional redundancy. Here, we report that Nkx6.3 expression is specific to Chx10+ V2a interneurons but not to Gata3+ V2b interneurons of the hindbrain, and that Nkx6.3 expression appears to mark cells of the prospective medullary reticular formation. Molecular analysis of Nkx6.3 null embryonic mouse hindbrain did not reveal detectable defects in progenitor markers, motor neuron or V2 interneuron sub-types. Forced expression of Nkx6.3 and Nkx6.1 promote V2 interneuron differentiation in the developing chick hindbrain. These findings indicate Nkx6.3 function is dispensable for CNS development and lead to the proposal that absence of overt defects is due to functional compensation from a related homeodomain transcription factor.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>18586225</pmid><doi>10.1016/j.brainres.2008.04.072</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Brain research, 2008-07, Vol.1222, p.42-50 |
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| subjects | Animals Biochemistry and metabolism Biological and medical sciences Central nervous system CNS development Electroporation - methods Embryo, Mammalian Eye Proteins - genetics Eye Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression - physiology Gene Expression Regulation, Developmental - genetics Homeobox transcription factor Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Homeodomain Proteins - physiology Interneurons - metabolism LIM-Homeodomain Proteins Medullary reticular formation Mice Mice, Inbred C57BL Mice, Mutant Strains Mutation Neurology Nkx6.1 Nkx6.3 Paired Box Transcription Factors - genetics Paired Box Transcription Factors - metabolism PAX6 Transcription Factor Repressor Proteins - genetics Repressor Proteins - metabolism Rhombencephalon - cytology Rhombencephalon - embryology Rhombencephalon - metabolism Rhombomere Transcription Factors - genetics Transcription Factors - metabolism Transcription Factors - physiology V2 interneuron Vertebrates: nervous system and sense organs |
| title | Expression and function of Nkx6.3 in vertebrate hindbrain |
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