Sensitized-erythrocyte-lysis (SEL) test as an epidemiological tool for human leptospirosis serological surveys
Epidemiological studies of human leptospirosis have generally been limited to countries with specialized laboratories employing the microscopic-agglutination (MA) test. The sensitized-erythrocyte-lysis (SEL) test is much simpler for routine hospital laboratories to carry out and it has been found va...
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| Veröffentlicht in: | Bulletin of the World Health Organization 1969, Vol.40 (6), p.899-902 |
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| creator | Tan, D S |
| description | Epidemiological studies of human leptospirosis have generally been limited to countries with specialized laboratories employing the microscopic-agglutination (MA) test. The sensitized-erythrocyte-lysis (SEL) test is much simpler for routine hospital laboratories to carry out and it has been found valuable in the diagnosis of human leptospirosis. This paper reports the results of studies of the SEL test as an epidemiological tool in serological surveys.The results showed that the significant SEL titre was 1:80 and that the sensitivity of the test depended possibly on the antigen preparation and the amount of complement used. Most of the SEL antibodies were found to persist at significant titres for about 1 year after active infection, but less than half persisted longer than that. The SEL test is therefore useful for detecting recent infections and for indicating that stability of leptospirosis in an area.The endemicity of leptospirosis in West Malaysia was confirmed by the SEL test, based on the employment of 1:80 as the significant titre. |
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The sensitized-erythrocyte-lysis (SEL) test is much simpler for routine hospital laboratories to carry out and it has been found valuable in the diagnosis of human leptospirosis. This paper reports the results of studies of the SEL test as an epidemiological tool in serological surveys.The results showed that the significant SEL titre was 1:80 and that the sensitivity of the test depended possibly on the antigen preparation and the amount of complement used. Most of the SEL antibodies were found to persist at significant titres for about 1 year after active infection, but less than half persisted longer than that. The SEL test is therefore useful for detecting recent infections and for indicating that stability of leptospirosis in an area.The endemicity of leptospirosis in West Malaysia was confirmed by the SEL test, based on the employment of 1:80 as the significant titre.</description><identifier>ISSN: 0042-9686</identifier><identifier>PMID: 5307602</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Antibodies - analysis ; Antibody Formation ; Antigens ; Epidemiologic Methods ; Erythrocytes - immunology ; Hemagglutination Tests ; Humans ; Immune Sera ; Leptospira - immunology ; Leptospirosis - diagnosis ; Leptospirosis - immunology ; Malaysia ; Serologic Tests</subject><ispartof>Bulletin of the World Health Organization, 1969, Vol.40 (6), p.899-902</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2554754/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2554754/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5307602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, D S</creatorcontrib><title>Sensitized-erythrocyte-lysis (SEL) test as an epidemiological tool for human leptospirosis serological surveys</title><title>Bulletin of the World Health Organization</title><addtitle>Bull World Health Organ</addtitle><description>Epidemiological studies of human leptospirosis have generally been limited to countries with specialized laboratories employing the microscopic-agglutination (MA) test. The sensitized-erythrocyte-lysis (SEL) test is much simpler for routine hospital laboratories to carry out and it has been found valuable in the diagnosis of human leptospirosis. This paper reports the results of studies of the SEL test as an epidemiological tool in serological surveys.The results showed that the significant SEL titre was 1:80 and that the sensitivity of the test depended possibly on the antigen preparation and the amount of complement used. Most of the SEL antibodies were found to persist at significant titres for about 1 year after active infection, but less than half persisted longer than that. The SEL test is therefore useful for detecting recent infections and for indicating that stability of leptospirosis in an area.The endemicity of leptospirosis in West Malaysia was confirmed by the SEL test, based on the employment of 1:80 as the significant titre.</description><subject>Antibodies - analysis</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Epidemiologic Methods</subject><subject>Erythrocytes - immunology</subject><subject>Hemagglutination Tests</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Leptospira - immunology</subject><subject>Leptospirosis - diagnosis</subject><subject>Leptospirosis - immunology</subject><subject>Malaysia</subject><subject>Serologic Tests</subject><issn>0042-9686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLxDAUhbNQxnH0JwhZiS4KaV6dbAQZfMGAi9F1SNvbmUja1CQdqL_eisOgd3MvnHO_A-cEzQnhNFNyKc_QeYwfZBrFyQzNBCOFJHSOug100Sb7BXUGYUy74KsxQebGaCO-2Tysb3GCmLCJ2HQYeltDa73zW1sZh5P3Djc-4N3QTrKDPvnY2-B_viOEozEOYQ9jvECnjXERLg97gd4fH95Wz9n69elldb_OeirzlCkmSs4aUtG8hqZUUkEhGVeFqTiI6aa1gYLmOZG8ZkqVSyoFl5SaIldgJFugu19uP5Qt1BV0KRin-2BbE0btjdX_lc7u9NbvNRWCF4JPgOsDIPjPYSpAtzZW4JzpwA9RLzkjlBM2Ga_-Jh0jDg2zb04qecw</recordid><startdate>1969</startdate><enddate>1969</enddate><creator>Tan, D S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1969</creationdate><title>Sensitized-erythrocyte-lysis (SEL) test as an epidemiological tool for human leptospirosis serological surveys</title><author>Tan, D S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p261t-935b43f0c21defb969e763497ac4e5e762dae7211064d399b82654622a719ea63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Antibodies - analysis</topic><topic>Antibody Formation</topic><topic>Antigens</topic><topic>Epidemiologic Methods</topic><topic>Erythrocytes - immunology</topic><topic>Hemagglutination Tests</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Leptospira - immunology</topic><topic>Leptospirosis - diagnosis</topic><topic>Leptospirosis - immunology</topic><topic>Malaysia</topic><topic>Serologic Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, D S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bulletin of the World Health Organization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, D S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitized-erythrocyte-lysis (SEL) test as an epidemiological tool for human leptospirosis serological surveys</atitle><jtitle>Bulletin of the World Health Organization</jtitle><addtitle>Bull World Health Organ</addtitle><date>1969</date><risdate>1969</risdate><volume>40</volume><issue>6</issue><spage>899</spage><epage>902</epage><pages>899-902</pages><issn>0042-9686</issn><abstract>Epidemiological studies of human leptospirosis have generally been limited to countries with specialized laboratories employing the microscopic-agglutination (MA) test. The sensitized-erythrocyte-lysis (SEL) test is much simpler for routine hospital laboratories to carry out and it has been found valuable in the diagnosis of human leptospirosis. This paper reports the results of studies of the SEL test as an epidemiological tool in serological surveys.The results showed that the significant SEL titre was 1:80 and that the sensitivity of the test depended possibly on the antigen preparation and the amount of complement used. Most of the SEL antibodies were found to persist at significant titres for about 1 year after active infection, but less than half persisted longer than that. The SEL test is therefore useful for detecting recent infections and for indicating that stability of leptospirosis in an area.The endemicity of leptospirosis in West Malaysia was confirmed by the SEL test, based on the employment of 1:80 as the significant titre.</abstract><cop>Switzerland</cop><pmid>5307602</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Bulletin of the World Health Organization, 1969, Vol.40 (6), p.899-902 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antibodies - analysis Antibody Formation Antigens Epidemiologic Methods Erythrocytes - immunology Hemagglutination Tests Humans Immune Sera Leptospira - immunology Leptospirosis - diagnosis Leptospirosis - immunology Malaysia Serologic Tests |
| title | Sensitized-erythrocyte-lysis (SEL) test as an epidemiological tool for human leptospirosis serological surveys |
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