Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation
To understand the molecular mechanism underlying vigorous proliferative activity of hepatic stem-like (HSL) cells, we performed two-dimensional electrophoresis to identify the proteins statistically more abundant in rapidly growing undifferentiated HSL cells than in sodium butyrate-treated different...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2008-06, Vol.57 (2), p.137-143 |
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| creator | Saheki, Toshihiko Ito, Hitomi Sekiguchi, Akihiro Nishina, Atsuyoshi Sugiyama, Toshihiro Izumi, Takashi Kojima, Itaru |
| description | To understand the molecular mechanism underlying vigorous proliferative activity of hepatic stem-like (HSL) cells, we performed two-dimensional electrophoresis to identify the proteins statistically more abundant in rapidly growing undifferentiated HSL cells than in sodium butyrate-treated differentiated HSL cells. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry and Mascot search identified 6 proteins including prohibitin, vimentin, ezrin, annexin A3, acidic ribosomal phosphoprotein P0 and Grp75. Prohibitin and vimentin control the mitogen-activated protein (MAP) kinase pathway. Ezrin is phosphorylated by various protein-tyrosine kinases and modulates interactions between cytoskeletal and membrane proteins. Annexin A3 has a role in DNA synthesis. Acidic ribosomal phosphoprotein P0 and Grp75 play in protein synthesis. These results suggest that the proteins related to the MAP kinase cascade had some role in continuous proliferation of HSL cells without differentiation. |
| doi_str_mv | 10.1007/s10616-008-9122-7 |
| format | Article |
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These results suggest that the proteins related to the MAP kinase cascade had some role in continuous proliferation of HSL cells without differentiation.</description><subject>Acids</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytoskeleton</subject><subject>DNA biosynthesis</subject><subject>Ezrin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>JAACT Special Issue</subject><subject>Liver</subject><subject>MAP kinase</subject><subject>Mass spectroscopy</subject><subject>Membrane proteins</subject><subject>Molecular modelling</subject><subject>Peptides</subject><subject>Polymerase chain reaction</subject><subject>Prohibitin</subject><subject>Protein biosynthesis</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Sodium butyrate</subject><subject>Software</subject><subject>Stem cells</subject><subject>Vimentin</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU-LFDEQxYMo7uzoB_AiAZE9tVYlnaRzEZbFf7CgBz2HdHd6Jmt3Z0zSLvvtTTPDrgqeAlW_enlVj5AXCG8QQL1NCBJlBdBUGhmr1COyQaF4BUo1j8kGNINKg9Rn5DylGwDQCvlTcoYagKNoNmT8GkN2YfIdtbMd75JP1Pduzn7wLtHD2vVzonlvM-1Cqc-LoznQXQy3dO8ONpfRlN1Ujf6Ho50bx0Rvfd6HJdPeD4OLq1rBwvyMPBnsmNzz07sl3z-8_3b1qbr-8vHz1eV11dVa56rtW1VLy21vLSjGHYMeUEvd1kIogWV3B2JwXPCeYTvUHJXshgZ4L2pW93xL3h11D0s7ub4rBqIdzSH6ycY7E6w3f3dmvze78MswIbiUsghcnARi-Lm4lM3k07qanV1YklGcN6qGcuotefUPeROWWC6ZDNPYMIkNNoXCI9XFkFJ0w70XBLNGaY5RmhKlWaM0q_LLP5d4mDhlV4DXJ8Cmzo5DtHPn0z3HQApVaywcO3KptOadiw8W___7b_iDuJU</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Saheki, Toshihiko</creator><creator>Ito, Hitomi</creator><creator>Sekiguchi, Akihiro</creator><creator>Nishina, Atsuyoshi</creator><creator>Sugiyama, Toshihiro</creator><creator>Izumi, Takashi</creator><creator>Kojima, Itaru</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080601</creationdate><title>Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation</title><author>Saheki, Toshihiko ; Ito, Hitomi ; Sekiguchi, Akihiro ; Nishina, Atsuyoshi ; Sugiyama, Toshihiro ; Izumi, Takashi ; Kojima, Itaru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-bdb746a3adaa0723e20d01969b455751100e05fe353d21bf43176cf803d5424d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acids</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytoskeleton</topic><topic>DNA biosynthesis</topic><topic>Ezrin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>JAACT Special Issue</topic><topic>Liver</topic><topic>MAP kinase</topic><topic>Mass spectroscopy</topic><topic>Membrane proteins</topic><topic>Molecular modelling</topic><topic>Peptides</topic><topic>Polymerase chain reaction</topic><topic>Prohibitin</topic><topic>Protein biosynthesis</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Sodium butyrate</topic><topic>Software</topic><topic>Stem cells</topic><topic>Vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saheki, Toshihiko</creatorcontrib><creatorcontrib>Ito, Hitomi</creatorcontrib><creatorcontrib>Sekiguchi, Akihiro</creatorcontrib><creatorcontrib>Nishina, Atsuyoshi</creatorcontrib><creatorcontrib>Sugiyama, Toshihiro</creatorcontrib><creatorcontrib>Izumi, Takashi</creatorcontrib><creatorcontrib>Kojima, Itaru</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saheki, Toshihiko</au><au>Ito, Hitomi</au><au>Sekiguchi, Akihiro</au><au>Nishina, Atsuyoshi</au><au>Sugiyama, Toshihiro</au><au>Izumi, Takashi</au><au>Kojima, Itaru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>57</volume><issue>2</issue><spage>137</spage><epage>143</epage><pages>137-143</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>To understand the molecular mechanism underlying vigorous proliferative activity of hepatic stem-like (HSL) cells, we performed two-dimensional electrophoresis to identify the proteins statistically more abundant in rapidly growing undifferentiated HSL cells than in sodium butyrate-treated differentiated HSL cells. 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| subjects | Acids Biochemistry Biological and medical sciences Biomedicine Biotechnology Cell differentiation Cell proliferation Chemistry Chemistry and Materials Science Cytoskeleton DNA biosynthesis Ezrin Fundamental and applied biological sciences. Psychology JAACT Special Issue Liver MAP kinase Mass spectroscopy Membrane proteins Molecular modelling Peptides Polymerase chain reaction Prohibitin Protein biosynthesis Proteins Proteomics Sodium butyrate Software Stem cells Vimentin |
| title | Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation |
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