Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines
Allium hirtifolim (Persian Shallot) belongs to Allium genus ( Alliaceae family). We investigated the in vitro effects of chloroformic extract of A. hirtifolium and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, con...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2008-03, Vol.56 (3), p.179-185 |
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| creator | Ghodrati Azadi, Hamideh Ghaffari, Seyed Mahmood Riazi, Gholam Hossein Ahmadian, Shahin Vahedi, Fatemeh |
| description | Allium hirtifolim
(Persian Shallot) belongs to
Allium
genus (
Alliaceae
family). We investigated the in vitro effects of chloroformic extract of
A. hirtifolium
and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, connective) cell lines. Our results showed that components of
A. hirtifolium
might inhibit proliferation of tumor cell lines. This inhibition in HeLa and MCF-7 cells was dose-dependent. The presence of Allicin was evaluated by TLC method in bulbs and the extract of
A. hirtifolium
was analyzed by HPLC. MTT test was performed 24, 48 and 72 h after cell culture. A significant decrease in cell lines was observed in HeLa and MCF-7 as compared to L929 cell lines. DNA fragmentation analysis revealed a large number of apoptotic cells in treated HeLa and MCF-7 cell groups, but no effects in L929 cells. Therefore
A. hirtifolium
might be a candidate for tumor suppression. |
| doi_str_mv | 10.1007/s10616-008-9145-0 |
| format | Article |
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(Persian Shallot) belongs to
Allium
genus (
Alliaceae
family). We investigated the in vitro effects of chloroformic extract of
A. hirtifolium
and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, connective) cell lines. Our results showed that components of
A. hirtifolium
might inhibit proliferation of tumor cell lines. This inhibition in HeLa and MCF-7 cells was dose-dependent. The presence of Allicin was evaluated by TLC method in bulbs and the extract of
A. hirtifolium
was analyzed by HPLC. MTT test was performed 24, 48 and 72 h after cell culture. A significant decrease in cell lines was observed in HeLa and MCF-7 as compared to L929 cell lines. DNA fragmentation analysis revealed a large number of apoptotic cells in treated HeLa and MCF-7 cell groups, but no effects in L929 cells. Therefore
A. hirtifolium
might be a candidate for tumor suppression.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-008-9145-0</identifier><identifier>PMID: 19002856</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adenocarcinoma ; Allium ; Apoptosis ; Biochemistry ; Biological and medical sciences ; Biomedicine ; Biotechnology ; Cancer ; Cell culture ; Cell proliferation ; Cervical cancer ; Chemistry ; Chemistry and Materials Science ; DNA fragmentation ; Fundamental and applied biological sciences. Psychology ; Garlic ; Morphology ; Original Research ; Tumor cell lines ; Tumor suppression ; Tumors</subject><ispartof>Cytotechnology (Dordrecht), 2008-03, Vol.56 (3), p.179-185</ispartof><rights>Springer Science+Business Media B.V. 2008</rights><rights>2008 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2008.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-25224a88a6a750d6babb0b55a162dc8738e0afd71ceeeeda87cf887a1082b6a3</citedby><cites>FETCH-LOGICAL-c565t-25224a88a6a750d6babb0b55a162dc8738e0afd71ceeeeda87cf887a1082b6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553628/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918264987?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,21367,27901,27902,33721,33722,41464,42533,43781,51294,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20519233$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19002856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghodrati Azadi, Hamideh</creatorcontrib><creatorcontrib>Ghaffari, Seyed Mahmood</creatorcontrib><creatorcontrib>Riazi, Gholam Hossein</creatorcontrib><creatorcontrib>Ahmadian, Shahin</creatorcontrib><creatorcontrib>Vahedi, Fatemeh</creatorcontrib><title>Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>Allium hirtifolim
(Persian Shallot) belongs to
Allium
genus (
Alliaceae
family). We investigated the in vitro effects of chloroformic extract of
A. hirtifolium
and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, connective) cell lines. Our results showed that components of
A. hirtifolium
might inhibit proliferation of tumor cell lines. This inhibition in HeLa and MCF-7 cells was dose-dependent. The presence of Allicin was evaluated by TLC method in bulbs and the extract of
A. hirtifolium
was analyzed by HPLC. MTT test was performed 24, 48 and 72 h after cell culture. A significant decrease in cell lines was observed in HeLa and MCF-7 as compared to L929 cell lines. DNA fragmentation analysis revealed a large number of apoptotic cells in treated HeLa and MCF-7 cell groups, but no effects in L929 cells. Therefore
A. hirtifolium
might be a candidate for tumor suppression.</description><subject>Adenocarcinoma</subject><subject>Allium</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cell culture</subject><subject>Cell proliferation</subject><subject>Cervical cancer</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>DNA fragmentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Garlic</subject><subject>Morphology</subject><subject>Original Research</subject><subject>Tumor cell lines</subject><subject>Tumor suppression</subject><subject>Tumors</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kW1rFDEQx4Mo9nr6AXwjAZG-6eoke3nYN4Wj-AQFBfs-zOayvZRscia7xX57c97RqmAgzJD5zWT-_Al5xeAdA1DvCwPJZAOgm46tRANPyIIJ1TaglH5KFtBxaDqQ3Qk5LeUWADrF2ufkhHUAXAu5IHEdJ7_LKfjBZZz8naNoa_DTPU0DtduQchpSHr2l7ueUa3H__s3l4jHS71sMIU3ndB2Cn0e69XnyQ9rn5zRFOs1jytS6EGjw0ZUX5NmAobiXx7gk1x8_XF9-bq6-fvpyub5qrJBiarjgfIVao0QlYCN77HvohUAm-cZq1WoHOGwUs66eDWplB60VMtC8l9guycVh7G7uR7exLtbNg9llP2K-Nwm9-bsS_dbcpDvDhWgl13XA2XFATj9mVyYz-rKXgdGluRjVtvpwl-TNP-RtmnOs4gzvmOZy1f2m2IGyOZWS3fCwCwOz99IcvDTVS7P30kDtef2niMeOo3kVeHsEsFgMQ8ZofXngOAjW8batHD9wpZbijcuPK_7_91-RhLm5</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Ghodrati Azadi, Hamideh</creator><creator>Ghaffari, Seyed Mahmood</creator><creator>Riazi, Gholam Hossein</creator><creator>Ahmadian, Shahin</creator><creator>Vahedi, Fatemeh</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080301</creationdate><title>Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines</title><author>Ghodrati Azadi, Hamideh ; Ghaffari, Seyed Mahmood ; Riazi, Gholam Hossein ; Ahmadian, Shahin ; Vahedi, Fatemeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-25224a88a6a750d6babb0b55a162dc8738e0afd71ceeeeda87cf887a1082b6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adenocarcinoma</topic><topic>Allium</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cell culture</topic><topic>Cell proliferation</topic><topic>Cervical cancer</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>DNA fragmentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Garlic</topic><topic>Morphology</topic><topic>Original Research</topic><topic>Tumor cell lines</topic><topic>Tumor suppression</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghodrati Azadi, Hamideh</creatorcontrib><creatorcontrib>Ghaffari, Seyed Mahmood</creatorcontrib><creatorcontrib>Riazi, Gholam Hossein</creatorcontrib><creatorcontrib>Ahmadian, Shahin</creatorcontrib><creatorcontrib>Vahedi, Fatemeh</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghodrati Azadi, Hamideh</au><au>Ghaffari, Seyed Mahmood</au><au>Riazi, Gholam Hossein</au><au>Ahmadian, Shahin</au><au>Vahedi, Fatemeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>56</volume><issue>3</issue><spage>179</spage><epage>185</epage><pages>179-185</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>Allium hirtifolim
(Persian Shallot) belongs to
Allium
genus (
Alliaceae
family). We investigated the in vitro effects of chloroformic extract of
A. hirtifolium
and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, connective) cell lines. Our results showed that components of
A. hirtifolium
might inhibit proliferation of tumor cell lines. This inhibition in HeLa and MCF-7 cells was dose-dependent. The presence of Allicin was evaluated by TLC method in bulbs and the extract of
A. hirtifolium
was analyzed by HPLC. MTT test was performed 24, 48 and 72 h after cell culture. A significant decrease in cell lines was observed in HeLa and MCF-7 as compared to L929 cell lines. DNA fragmentation analysis revealed a large number of apoptotic cells in treated HeLa and MCF-7 cell groups, but no effects in L929 cells. Therefore
A. hirtifolium
might be a candidate for tumor suppression.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>19002856</pmid><doi>10.1007/s10616-008-9145-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SpringerLink Journals - AutoHoldings; ProQuest Central |
| subjects | Adenocarcinoma Allium Apoptosis Biochemistry Biological and medical sciences Biomedicine Biotechnology Cancer Cell culture Cell proliferation Cervical cancer Chemistry Chemistry and Materials Science DNA fragmentation Fundamental and applied biological sciences. Psychology Garlic Morphology Original Research Tumor cell lines Tumor suppression Tumors |
| title | Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines |
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