Vibrational Stark Effect Probes for Nucleic Acids

Vibrational Stark Effect Probes for Nucleic Acids

The vibrational Stark effect (VSE) has proven to be an effective method for the study of electric fields in proteins via the use of infrared probes. To explore the use of VSE in nucleic acids, we investigated the Stark spectroscopy of nine structurally diverse nucleosides. These nucleosides containe...

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Veröffentlicht in:The journal of physical chemistry. B 2007-10, Vol.111 (40), p.11611-11613
Hauptverfasser: Silverman, Lisa N, Pitzer, Michael E, Ankomah, Peter O, Boxer, Steven G, Fenlon, Edward E
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Sprache:eng
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DNA - chemistry
Molecular Probe Techniques
Molecular Probes
Nitriles
Nucleosides
RNA - chemistry
Static Electricity
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container_end_page 11613
container_issue 40
container_start_page 11611
container_title The journal of physical chemistry. B
container_volume 111
creator Silverman, Lisa N
Pitzer, Michael E
Ankomah, Peter O
Boxer, Steven G
Fenlon, Edward E
description The vibrational Stark effect (VSE) has proven to be an effective method for the study of electric fields in proteins via the use of infrared probes. To explore the use of VSE in nucleic acids, we investigated the Stark spectroscopy of nine structurally diverse nucleosides. These nucleosides contained nitrile or azide probes in positions that correspond to both the major and minor grooves of DNA. The nitrile probes showed better characteristics and exhibited absorption frequencies over a broad range; that is, from 2253 cm-1 for 2‘-O-cyanoethyl ribonucleosides 8 and 9 to 2102 cm-1 for a 13C-labeled 5-thiocyanatomethyl-2‘-deoxyuridine 3c. The largest Stark tuning rate observed was |Δμ| = 1.1 cm-1/(MV/cm) for both 5-cyano-2‘-deoxyuridine 1 and N2-nitrile-2‘-deoxyguanosine 7. The latter is a particularly attractive probe because of its high extinction coefficient (ε = 412 M-1cm-1) and ease of incorporation into oligomers.
doi_str_mv 10.1021/jp0750912
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subjects DNA - chemistry
Molecular Probe Techniques
Molecular Probes
Nitriles
Nucleosides
RNA - chemistry
Static Electricity
title Vibrational Stark Effect Probes for Nucleic Acids
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