Molecular Assembly of Superquenchers in Signaling Molecular Interactions
We have designed a novel molecular assembly of quencher molecules to form superquenchers with excellent quenching efficiency. The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a...
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| Veröffentlicht in: | Journal of the American Chemical Society 2005-09, Vol.127 (37), p.12772-12773 |
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| container_end_page | 12773 |
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| container_issue | 37 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 127 |
| creator | Yang, Chaoyong James Lin, Hui Tan, Weihong |
| description | We have designed a novel molecular assembly of quencher molecules to form superquenchers with excellent quenching efficiency. The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a 320-fold enhancement of fluorescent signal was achieved, compared to about 14-fold from a molecular beacon prepared with the same monomer quencher. Our molecular assembly approach can effectively improve the sensitivity of a variety of fluorescent assays and can be widely useful for molecular interaction studies. |
| doi_str_mv | 10.1021/ja053482t |
| format | Article |
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The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a 320-fold enhancement of fluorescent signal was achieved, compared to about 14-fold from a molecular beacon prepared with the same monomer quencher. Our molecular assembly approach can effectively improve the sensitivity of a variety of fluorescent assays and can be widely useful for molecular interaction studies.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja053482t</identifier><identifier>PMID: 16159250</identifier><identifier>CODEN: JACSAT</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; DNA Probes - chemistry ; Fluorescent Dyes - chemistry ; Fundamental and applied biological sciences. Psychology ; Interactions. 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Associations</subject><subject>Intermolecular phenomena</subject><subject>Molecular biophysics</subject><subject>Molecular Probe Techniques</subject><subject>Molecular Structure</subject><subject>Oligonucleotides - chemistry</subject><subject>Photochemistry. Photosynthesis. Bioluminescence</subject><subject>Radiation-biomolecule interaction</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Temperature</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1vEzEQhi0EomnhwB9AewGJw4LH37kgtYF-oKIWUs6W1_WmDo4d7N2K_ntcJUpA4jQazTPvzLyD0CvA7wET-LA0mFOmyPAETYAT3HIg4imaYIxJK5WgB-iwlGVNGVHwHB2AAD4lHE_Q-dcUnB2Dyc1xKW7VhYcm9c18XLv8a3TR3rlcGh-buV9EE3xcNPuOizi4bOzgUywv0LPehOJebuMR-nH6-WZ23l5enV3Mji9bw6kcWuFsRwUwSTvgxhBKp8AUCEvBYdXfYiYtI5xjEIwIxaSwHRad6KdqSjDr6BH6uNFdj93K3VoXh2yCXme_MvlBJ-P1v5Xo7_Qi3WvCqRBKVoG3W4Gc6oVl0CtfrAvBRJfGooXiEjNKK_huA9qcSsmu3w0BrB991zvfK_v676325NboCrzZAqZYE_psovVlz0kAWk-uXLvhfBnc713d5J9aSCq5vrme69kpfPkkv5_ob3tdY4tepjHXJ5X_LPgHiIilfA</recordid><startdate>20050921</startdate><enddate>20050921</enddate><creator>Yang, Chaoyong James</creator><creator>Lin, Hui</creator><creator>Tan, Weihong</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050921</creationdate><title>Molecular Assembly of Superquenchers in Signaling Molecular Interactions</title><author>Yang, Chaoyong James ; Lin, Hui ; Tan, Weihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a537t-6ecb361473b15aa233914816c31e08fd047c42550164268476cb06b6f989204b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>DNA Probes - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Interactions. Associations</topic><topic>Intermolecular phenomena</topic><topic>Molecular biophysics</topic><topic>Molecular Probe Techniques</topic><topic>Molecular Structure</topic><topic>Oligonucleotides - chemistry</topic><topic>Photochemistry. Photosynthesis. Bioluminescence</topic><topic>Radiation-biomolecule interaction</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Chaoyong James</creatorcontrib><creatorcontrib>Lin, Hui</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Chaoyong James</au><au>Lin, Hui</au><au>Tan, Weihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Assembly of Superquenchers in Signaling Molecular Interactions</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2005-09-21</date><risdate>2005</risdate><volume>127</volume><issue>37</issue><spage>12772</spage><epage>12773</epage><pages>12772-12773</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>We have designed a novel molecular assembly of quencher molecules to form superquenchers with excellent quenching efficiency. The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a 320-fold enhancement of fluorescent signal was achieved, compared to about 14-fold from a molecular beacon prepared with the same monomer quencher. 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| ispartof | Journal of the American Chemical Society, 2005-09, Vol.127 (37), p.12772-12773 |
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| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Biological and medical sciences DNA Probes - chemistry Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology Interactions. Associations Intermolecular phenomena Molecular biophysics Molecular Probe Techniques Molecular Structure Oligonucleotides - chemistry Photochemistry. Photosynthesis. Bioluminescence Radiation-biomolecule interaction Spectrometry, Fluorescence - methods Temperature |
| title | Molecular Assembly of Superquenchers in Signaling Molecular Interactions |
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