Activator-dependent recruitment of SWI/SNF and INO80 during INO1 activation
Transcriptional activation of yeast INO1 requires SWI/SNF and INO80 for nucleosome disruption at the promoter. However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SW...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-09, Vol.373 (4), p.602-606 |
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| creator | Ford, Jason Odeyale, Oluwafemi Shen, Chang-Hui |
| description | Transcriptional activation of yeast INO1 requires SWI/SNF and INO80 for nucleosome disruption at the promoter. However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SWI/SNF and INO80 are present at the promoter and are restricted to the promoter, indicating that they directly participate in localized INO1 chromatin remodeling. Furthermore, both SWI/SNF and INO80 are absent at the INO1 promoter in ino2Δ cells, suggesting that these are activator-dependent remodelers. We have also found that the presence of INO80 is required for SWI/SNF recruitment, indicating that INO80 arrives first at the promoter followed by SWI/SNF. In light of these findings, we proposed a model which describes the order of events in INO1 activation. |
| doi_str_mv | 10.1016/j.bbrc.2008.06.079 |
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However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SWI/SNF and INO80 are present at the promoter and are restricted to the promoter, indicating that they directly participate in localized INO1 chromatin remodeling. Furthermore, both SWI/SNF and INO80 are absent at the INO1 promoter in ino2Δ cells, suggesting that these are activator-dependent remodelers. We have also found that the presence of INO80 is required for SWI/SNF recruitment, indicating that INO80 arrives first at the promoter followed by SWI/SNF. 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However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SWI/SNF and INO80 are present at the promoter and are restricted to the promoter, indicating that they directly participate in localized INO1 chromatin remodeling. Furthermore, both SWI/SNF and INO80 are absent at the INO1 promoter in ino2Δ cells, suggesting that these are activator-dependent remodelers. We have also found that the presence of INO80 is required for SWI/SNF recruitment, indicating that INO80 arrives first at the promoter followed by SWI/SNF. In light of these findings, we proposed a model which describes the order of events in INO1 activation.</description><subject>Adenosine Triphosphatases</subject><subject>ChIP</subject><subject>Chromatin Assembly and Disassembly</subject><subject>Chromatin Immunoprecipitation</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene activation</subject><subject>Gene Expression Regulation, Fungal</subject><subject>INO1</subject><subject>INO2</subject><subject>INO80</subject><subject>Local nucleosome movement</subject><subject>Microfilament Proteins - metabolism</subject><subject>Myo-Inositol-1-Phosphate Synthase - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>SNF2</subject><subject>SWI/SNF</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1vEzEQhi0EoqHwBzigPXHb7Yy_spYQUlW1EFG1h4LgZnntSXGUrIO9G4l_z64S8XHhNB75nWdG78vYa4QGAfXFpum67BsO0DagG1iaJ2yBYKDmCPIpWwCArrnBb2fsRSkbAESpzXN2hq0yQmmzYJ8u_RAPbki5DrSnPlA_VJl8HuOwm99pXT18XV083N1Urg_V6u6-hSqMOfaPc4OVOwJi6l-yZ2u3LfTqVM_Zl5vrz1cf69v7D6ury9vaSyWGWgovOkE-cOdRK7PsFHoycsm99BhUUIIHRYpEaBHX2AWJCqjlSgJ6sxTn7P2Rux-7HQU_nZnd1u5z3Ln80yYX7b8_ffxuH9PBciW0bGfA2xMgpx8jlcHuYvG03bqe0lisNkIaLvkk5Eehz6mUTOvfSxDsnIHd2DkDO2dgQdspg2nozd_n_Rk5mT4J3h0FNJl0iJRt8ZF6TyFOzg82pPg__i9C8pfp</recordid><startdate>20080905</startdate><enddate>20080905</enddate><creator>Ford, Jason</creator><creator>Odeyale, Oluwafemi</creator><creator>Shen, Chang-Hui</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080905</creationdate><title>Activator-dependent recruitment of SWI/SNF and INO80 during INO1 activation</title><author>Ford, Jason ; Odeyale, Oluwafemi ; Shen, Chang-Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-43c3b3ecd2ac16597b51ce9472c4c1d5d532d5e5e3d811f1bd4150e825401c973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adenosine Triphosphatases</topic><topic>ChIP</topic><topic>Chromatin Assembly and Disassembly</topic><topic>Chromatin Immunoprecipitation</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene activation</topic><topic>Gene Expression Regulation, Fungal</topic><topic>INO1</topic><topic>INO2</topic><topic>INO80</topic><topic>Local nucleosome movement</topic><topic>Microfilament Proteins - metabolism</topic><topic>Myo-Inositol-1-Phosphate Synthase - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>SNF2</topic><topic>SWI/SNF</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ford, Jason</creatorcontrib><creatorcontrib>Odeyale, Oluwafemi</creatorcontrib><creatorcontrib>Shen, Chang-Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ford, Jason</au><au>Odeyale, Oluwafemi</au><au>Shen, Chang-Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activator-dependent recruitment of SWI/SNF and INO80 during INO1 activation</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-09-05</date><risdate>2008</risdate><volume>373</volume><issue>4</issue><spage>602</spage><epage>606</epage><pages>602-606</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Transcriptional activation of yeast INO1 requires SWI/SNF and INO80 for nucleosome disruption at the promoter. However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SWI/SNF and INO80 are present at the promoter and are restricted to the promoter, indicating that they directly participate in localized INO1 chromatin remodeling. Furthermore, both SWI/SNF and INO80 are absent at the INO1 promoter in ino2Δ cells, suggesting that these are activator-dependent remodelers. We have also found that the presence of INO80 is required for SWI/SNF recruitment, indicating that INO80 arrives first at the promoter followed by SWI/SNF. In light of these findings, we proposed a model which describes the order of events in INO1 activation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18593569</pmid><doi>10.1016/j.bbrc.2008.06.079</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Triphosphatases ChIP Chromatin Assembly and Disassembly Chromatin Immunoprecipitation DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene activation Gene Expression Regulation, Fungal INO1 INO2 INO80 Local nucleosome movement Microfilament Proteins - metabolism Myo-Inositol-1-Phosphate Synthase - genetics Promoter Regions, Genetic Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism SNF2 SWI/SNF Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcriptional Activation |
| title | Activator-dependent recruitment of SWI/SNF and INO80 during INO1 activation |
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