Tailoring Relaxation Dispersion Experiments for Fast-Associating Protein Complexes

NMR relaxation dispersion spectroscopy is a powerful technique to elucidate the mechanism of protein−protein binding reactions. However, it is difficult to optimize the concentration ratios that give relaxation dispersions of appropriate amplitude to determine accurate kinetic and thermodynamic para...

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Veröffentlicht in:	Journal of the American Chemical Society 2007-11, Vol.129 (44), p.13406-13407
Hauptverfasser:	Sugase, Kenji, Lansing, Jonathan C, Dyson, H. Jane, Wright, Peter E
Format:	Artikel
Sprache:	eng
Schlagworte:	CREB-Binding Protein - chemistry Hypoxia-Inducible Factor 1, alpha Subunit - chemistry Magnetic Resonance Spectroscopy - methods Nitrogen Isotopes Protein Binding Sensitivity and Specificity Time Factors Zinc Fingers
Online-Zugang:	Volltext
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