Rapid mass spectrometric analysis of 15N‐Leu incorporation fidelity during preparation of specifically labeled NMR samples

Advances in NMR spectroscopy have enabled the study of larger proteins that typically have significant overlap in their spectra. Specific 15N‐amino acid incorporation is a powerful tool for reducing spectral overlap and attaining reliable sequential assignments. However, scrambling of the label duri...

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Veröffentlicht in:	Protein science 2008-09, Vol.17 (9), p.1636-1639
Hauptverfasser:	Truhlar, Stephanie M.E., Cervantes, Carla F., Torpey, Justin W., Kjaergaard, Magnus, Komives, Elizabeth A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Ankyrin Repeat - genetics Electron Probe Microanalysis For the Record Hydrolysis I-kappa B Kinase - chemistry I-kappa B Kinase - genetics I-kappa B Kinase - isolation & purification I-kappa B Kinase - metabolism Isotope Labeling Leucine - metabolism MALDI mass spectrometry Molecular Sequence Data Mutation Nitrogen Isotopes NMR spectroscopy Nuclear Magnetic Resonance, Biomolecular Peptides - analysis Protein Structure, Tertiary proteolytic digestion specific 15N‐amino acid labeling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry Time Factors
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