Evaluation of in vitro cytotoxicity and paracellular permeability of intact monolayers with mouse embryonic stem cells

Mouse embryonic stem (mES) cells were induced to form intact monolayers in cell culture inserts, using combinations of extracellular matrix (ECM) components and growth factors (GFs). Progressive formation of intact monolayers was monitored using transepithelial electrical resistance (TEER) and passa...

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Veröffentlicht in:	Toxicology in vitro 2008-08, Vol.22 (5), p.1273-1284
Hauptverfasser:	Calabro, Anthony R., Konsoula, Roula, Barile, Frank A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques - methods Cell Line Cell Membrane Permeability - drug effects Cell Survival - drug effects Dose-Response Relationship, Drug Electric Impedance Embryonic Stem Cells - drug effects Embryonic Stem Cells - pathology Extracellular Matrix Proteins - metabolism Formazans - metabolism In vitro cytotoxicity Inhibitory Concentration 50 Intercellular Signaling Peptides and Proteins - metabolism Leukemia Inhibitory Factor - metabolism Mice Mouse embryonic stem cells MTT assay Paracellular permeability Tetrazolium Salts - metabolism Transepithelial electrical resistance Xenobiotics - metabolism Xenobiotics - toxicity
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