Cordon-Bleu Is an Actin Nucleation Factor and Controls Neuronal Morphology
Despite the wealth of different actin structures formed, only two actin nucleation factors are well established in vertebrates: the Arp2/3 complex and formins. Here, we describe a further nucleator, cordon-bleu (Cobl). Cobl is a brain-enriched protein using three Wiskott-Aldrich syndrome protein hom...
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| Veröffentlicht in: | Cell 2007-10, Vol.131 (2), p.337-350 |
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| creator | Ahuja, Rashmi Pinyol, Roser Reichenbach, Nicole Custer, Laura Klingensmith, John Kessels, Michael M. Qualmann, Britta |
| description | Despite the wealth of different actin structures formed, only two actin nucleation factors are well established in vertebrates: the Arp2/3 complex and formins. Here, we describe a further nucleator, cordon-bleu (Cobl). Cobl is a brain-enriched protein using three Wiskott-Aldrich syndrome protein homology 2 (WH2) domains for actin binding. Cobl promotes nonbundled, unbranched filaments. Filament formation relies on barbed-end growth and requires all three Cobl WH2 domains and the extended linker L2. We suggest that the nucleation power of Cobl is based on the assembly of three actin monomers in cross-filament orientation. Cobl localizes to sites of high actin dynamics and modulates cell morphology. In neurons, induction of both neurites and neurite branching is dramatically increased by Cobl expression—effects that critically depend on Cobl's actin nucleation ability. Correspondingly, Cobl depletion results in decreased dendritic arborization. Thus, Cobl is an actin nucleator controlling neuronal morphology and development. |
| doi_str_mv | 10.1016/j.cell.2007.08.030 |
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Here, we describe a further nucleator, cordon-bleu (Cobl). Cobl is a brain-enriched protein using three Wiskott-Aldrich syndrome protein homology 2 (WH2) domains for actin binding. Cobl promotes nonbundled, unbranched filaments. Filament formation relies on barbed-end growth and requires all three Cobl WH2 domains and the extended linker L2. We suggest that the nucleation power of Cobl is based on the assembly of three actin monomers in cross-filament orientation. Cobl localizes to sites of high actin dynamics and modulates cell morphology. In neurons, induction of both neurites and neurite branching is dramatically increased by Cobl expression—effects that critically depend on Cobl's actin nucleation ability. Correspondingly, Cobl depletion results in decreased dendritic arborization. Thus, Cobl is an actin nucleator controlling neuronal morphology and development.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2007.08.030</identifier><identifier>PMID: 17956734</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actin-Related Protein 2-3 Complex - physiology ; Actins - metabolism ; Amino Acid Sequence ; Animals ; cellbio ; Cells, Cultured ; Cercopithecus aethiops ; Humans ; Mice ; Molecular Sequence Data ; Neurites - physiology ; Neurons - cytology ; Neurons - metabolism ; Neurons - physiology ; Phylogeny ; Protein Structure, Tertiary ; Proteins - genetics ; Proteins - physiology ; Rats</subject><ispartof>Cell, 2007-10, Vol.131 (2), p.337-350</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-693090f02efcfb3ae4d6608f470dc37f457eb29580d3faa36133b7bd014bb71a3</citedby><cites>FETCH-LOGICAL-c550t-693090f02efcfb3ae4d6608f470dc37f457eb29580d3faa36133b7bd014bb71a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cell.2007.08.030$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17956734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahuja, Rashmi</creatorcontrib><creatorcontrib>Pinyol, Roser</creatorcontrib><creatorcontrib>Reichenbach, Nicole</creatorcontrib><creatorcontrib>Custer, Laura</creatorcontrib><creatorcontrib>Klingensmith, John</creatorcontrib><creatorcontrib>Kessels, Michael M.</creatorcontrib><creatorcontrib>Qualmann, Britta</creatorcontrib><title>Cordon-Bleu Is an Actin Nucleation Factor and Controls Neuronal Morphology</title><title>Cell</title><addtitle>Cell</addtitle><description>Despite the wealth of different actin structures formed, only two actin nucleation factors are well established in vertebrates: the Arp2/3 complex and formins. 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Here, we describe a further nucleator, cordon-bleu (Cobl). Cobl is a brain-enriched protein using three Wiskott-Aldrich syndrome protein homology 2 (WH2) domains for actin binding. Cobl promotes nonbundled, unbranched filaments. Filament formation relies on barbed-end growth and requires all three Cobl WH2 domains and the extended linker L2. We suggest that the nucleation power of Cobl is based on the assembly of three actin monomers in cross-filament orientation. Cobl localizes to sites of high actin dynamics and modulates cell morphology. In neurons, induction of both neurites and neurite branching is dramatically increased by Cobl expression—effects that critically depend on Cobl's actin nucleation ability. Correspondingly, Cobl depletion results in decreased dendritic arborization. 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| subjects | Actin-Related Protein 2-3 Complex - physiology Actins - metabolism Amino Acid Sequence Animals cellbio Cells, Cultured Cercopithecus aethiops Humans Mice Molecular Sequence Data Neurites - physiology Neurons - cytology Neurons - metabolism Neurons - physiology Phylogeny Protein Structure, Tertiary Proteins - genetics Proteins - physiology Rats |
| title | Cordon-Bleu Is an Actin Nucleation Factor and Controls Neuronal Morphology |
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